| Project/Area Number |
23656533
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | University of Hyogo |
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Principal Investigator |
NEGORO Seiji 兵庫県立大学, 工学(系)研究科(研究院), 教授 (90156159)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKEO Masahiro 兵庫県立大学, 大学院・工学研究科, 准教授 (40236443)
KATO Dai-ichiro 兵庫県立大学, 大学院・工学研究科, 助教 (60423901)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | ナイロン / 酵素 / 生分解 / 薄膜 / 加水分解 / 耐熱化酵素 / ガスクラスター２次イオン質量分析 / ガスクラスター二次イオン質量分析 |
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| Research Abstract |
Nylon hydrolase degrades various aliphatic nylons, including nylon-6 and nylon-66. We synthesized a nylon-66 copolymer, in which a part of an adipoyl unit of nylon-66 was replaced with a succinyl unit by interfacial polymerization. To quantify the reaction rate of the enzymatic hydrolysis of nylons at the surface of solid polymers, we prepared a thin-layer of nylons on the bottom surface of each well in a polystyrene-based micro-assay plate.
More than 99% of the copolymer with thicknesses of 260 nm were converted to water-soluble oligomers by nylon hydrolase. The degradation rate of the thin-layered nylon-6 was similarly analyzed. We demonstrate that this assay enables a quantitative evaluation of the reaction rate of hydrolysis at the interface between the solid and aqueous phases and a quantitative comparison of the degradability for various polyamides.
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