Biodegradation of nylon and its recycle
Project/Area Number |
23656533
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biofunction/Bioprocess
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Research Institution | University of Hyogo |
Principal Investigator |
NEGORO Seiji 兵庫県立大学, 工学(系)研究科(研究院), 教授 (90156159)
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Co-Investigator(Renkei-kenkyūsha) |
TAKEO Masahiro 兵庫県立大学, 大学院・工学研究科, 准教授 (40236443)
KATO Dai-ichiro 兵庫県立大学, 大学院・工学研究科, 助教 (60423901)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
|
Keywords | ナイロン / 酵素 / 生分解 / 薄膜 / 加水分解 / 耐熱化酵素 / ガスクラスター2次イオン質量分析 / ガスクラスター二次イオン質量分析 |
Research Abstract |
Nylon hydrolase degrades various aliphatic nylons, including nylon-6 and nylon-66. We synthesized a nylon-66 copolymer, in which a part of an adipoyl unit of nylon-66 was replaced with a succinyl unit by interfacial polymerization. To quantify the reaction rate of the enzymatic hydrolysis of nylons at the surface of solid polymers, we prepared a thin-layer of nylons on the bottom surface of each well in a polystyrene-based micro-assay plate. More than 99% of the copolymer with thicknesses of 260 nm were converted to water-soluble oligomers by nylon hydrolase. The degradation rate of the thin-layered nylon-6 was similarly analyzed. We demonstrate that this assay enables a quantitative evaluation of the reaction rate of hydrolysis at the interface between the solid and aqueous phases and a quantitative comparison of the degradability for various polyamides.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp2013
Author(s)
Nagai, K., Yasuhira, K., Tanaka, Y., Kato, D., Takeo, M., Higuchi, Y., Negoro, S., & Shibata N.
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Journal Title
KI72 Acta Cryst.
Volume: F69
Issue: 10
Pages: 1151-1154
DOI
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Peer Reviewed
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[Presentation] 高解像度X 線結晶構造解析を基盤としたナイロン加水分解酵素(NylC)の耐熱化機構の解明2013
Author(s)
衣笠 凌, 永井圭介, 三田隆二, 篠田昌宜, 柴田 直樹, 樋口芳樹, 李 映昊, 後藤祐児, 加藤太一郎, 武尾正弘, 根来誠司
Organizer
第65回日本生物工学会大会
Place of Presentation
広島国際会議場
Year and Date
2013-09-19
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[Presentation] アミド化合物の酵素合成 : 高効率触媒の形成要件2011
Author(s)
小林達也, 中田悠介, 馬場剛史, 河島康之, 柴田直樹, 加藤太一郎, 武尾正弘, 樋口芳樹, 重田育照, 松井亨, 神谷克政, 根来誠司
Organizer
第63回日本生物工学会大会
Place of Presentation
東京農工大学 (東京都)
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[Presentation] アシル化反応に対するアミノ酸変異の効果
Author(s)
馬場剛史, 神谷克政, 松井亨, 根来誠司, Mauro Boero, 中野雅由, 重田育照
Organizer
第10回京都大学福井謙一記念センターシンポジウム
Place of Presentation
京都大学 (京都府)
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