| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Research Abstract |
Surface proteins from Lactococcus lactis IL1403 cells purified by cellulose column were identified to be DnaK, GroEL, GAPDH and so on. The adsorption constants of the fluorescence labeled DnaK at pH 7.0 were estimated to be(2.7±0.9)×10^6、(2.5±0.5)×10^5、(3.7±2.2)×10^5、(8.7±3.2)×10^5 M^<-1> for cellulose, mucin, chitin and IL1403 cells, respectively. At pH 4.0, the adsorption constants for cellulose and mucin were estimated to be(5.7+-0.2) 10^6 and(4.4+-1.0) 10^6M^(-1), respectively. These results mean that lactic acid bacteria adhere to intestinal surface more tightly when they produce lactic acid.
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