Development of a new screening method to isolate the genes encoding RNA editing enzyme

• Project/Area Number: 23657003
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genetics/Genome dynamics
• Research Institution: Nagoya University
• Principal Investigator: SUGITA Mamoru 名古屋大学, 遺伝子実験施設, 教授 (70154474)
• Co-Investigator(Renkei-kenkyūsha): AOKI Setsuyuki 名古屋大学, 大学院・情報科学研究科, 准教授 (30283469)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: RNA 編集 / 葉緑体 / ミトコンドリア / ヒメツリガネゴケ / 逆遺伝学 / ペンタトリコペプチドリピートタンパク質 / PPRタンパク質 / RNA編集 / RNAスプライシング / RNAi法 / 葉緑体形質転換

Research Abstract

In chloroplasts and mitochondria, RNA editing frequently occurs in many transcripts of most terrestrial plants. RNA editing is an enigmatic phenomenon in which specific cytidines (C) in the transcripts are changed to uridines (U). Since C-to-U RNA editing was discoveredin plant organelles 20 years ago, the RNA editing enzyme or factors remain to be identified. In this study, we have identified several novel RNA editing factors using a model plant, Physcomitrella patens.

Research Products

• [Journal Article] CRUMPLED LEAF (CRL) Homologs of Physcomitrella patens are Involved in the Complete Separation of Dividing Plastids. (2012)
  • Author(s): Sugita C, et al.
  • Journal Title: Plant Cell Physiol. Volume: 53(6) Issue: 6 Pages: 1124-1133
  • DOI: 10.1093/pcp/pcs058
  • Peer Reviewed
• [Journal Article] A PPR-DYW protein is required for splicing of a group II intron of cox1pre-mRNA in Physcomitrella patens (2012)
  • Author(s): Ichinose, M., Tasaki, E., Sugita, C. Sugita, M
  • Journal Title: The Plant Journal Volume: 70(2) Issue: 2 Pages: 271-278
  • DOI: 10.1111/j.1365-313x.2011.04869.x
  • Peer Reviewed
• [Journal Article] Identification and characterization of the RNA binding surface of the pentatricopeptide repeat protein (2012)
  • Author(s): Keiko Kobayashi, Masuyo Kawabata, Keizo Hisano, Tomohiko Kazama, Ken Matsuoka, Mamoru Sugita, Takahiro Nakamura
  • Journal Title: Nucleic Acids Research Volume: (In press) Issue: 6 Pages: 2712-2723
  • DOI: 10.1093/nar/gkr1084
  • Peer Reviewed
• [Journal Article] The PPR-DYW proteins are required for RNA editing of rps14, cox1and nad5transcripts in Physcomitrella patens mitochondria (2012)
  • Author(s): Uchida, M., Ohtani, S., Ichinose, M., Sugita, C. Sugita, M
  • Journal Title: FEBS Letters Volume: 585(14) Issue: 14 Pages: 2367-2371
  • DOI: 10.1016/j.febslet.2011.06.009
  • Peer Reviewed
• [Journal Article] The Selaginella genome identifies genetic changes associated with the evolution of vascular plants (2011)
  • Author(s): Banks, et al
  • Journal Title: Science Volume: 332 Issue: 6032 Pages: 960-963
  • DOI: 10.1126/science.1203810
  • Peer Reviewed
• [Journal Article] DNA packaging proteins Glom and Glom2coordinately organize the mitochondrial nucleoid of Physarum polycephalum. (2011)
  • Author(s): Itoh, K., Izumi, A., Mori, T., Dohmae, N., Yui, R., Maeda-Sano, K., Shirai, Y., Kanaoka, M. M., Kuroiwa, T., Higashiyama, T., Sugita, M., Murakami-Murofushi, K., Kawano, S. and Sasaki, N
  • Journal Title: Mitochondrion Volume: 11(4) Pages: 575-586
  • Peer Reviewed
• [Presentation] ヒメツリガネゴケの新規PLS-type PPRタンパク質の同定と機能解析 (2013)
  • Author(s): 一瀬瑞穂,片山博文, 杉田護
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2013-03-23
• [Presentation] ヒメツリガネゴケのミトコンドリアRNA 編集因子の同定(3P-0740) (2012)
  • Author(s): 一瀬瑞穂、杉田護
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-13
• [Presentation] ヒメツリガネゴケを用いたRNA編集因子の同定 (2012)
  • Author(s): 一瀬瑞穂, 杉田 護
  • Organizer: 日本蘚苔類学会第41回北海道大会
  • Place of Presentation: 斜里町公民館ゆめホール知床
  • Year and Date: 2012-09-08
• [Presentation] The complete set of PPR-DYW proteins addressing all eleven editing sites in Physcomitrella patensmitochondrial transcripts (Poster and selected oral presentations) (2012)
  • Author(s): Mizuho Ichinose, Chieko Sugita, Mamoru Sugita
  • Organizer: Frontiers in Plant RNA Research 2012
  • Place of Presentation: Sapporo, Hokkaido
• [Presentation] ミトコンドリア機能不全はコケ原糸体のクロロネマ細胞からカウロネマ細胞への分化を抑制する (2012)
  • Author(s): 一瀬瑞穂, 杉田護
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
• [Presentation] ミトコンドリアのRNA編集に働くPPR-DYWタンパク質の同定(1pD14) (2011)
  • Author(s): 内田雅人, 大谷祥太郎,一瀬瑞穂,杉田千恵子,杉田護
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2011-09-17
• [Presentation] The moss PPR-DYW proteins are required for RNA editing and RNA splicing of mitochondrial transcripts. Oral presented on Oct. 16 (2011)
  • Author(s): Mamoru Sugita, Mizuho Ichinose , Shotaro Ohtani, Masato Uchida
  • Organizer: International Conference for Plant Mitochondrial Biology
  • Place of Presentation: Hohenroda, Germany
• [Presentation] The moss PPR-DYW proteins are required for RNA editing and RNA splicing of mitochondrial transcripts. (2011)
  • Author(s): Mamoru Sugita, Mizuho Ichinose, Shotaro Ohtani, Masato Uchida
  • Organizer: International Conference for Plant Mitochondrial Biology
  • Place of Presentation: Hohenroda, Germany
• [Presentation] ヒメツリガネゴケのPPR-DYWタンパク質はミトコンドリアcox1 pre-mRNAのスプライシングに働く (2011)
  • Author(s): 一瀬瑞穂, 田崎瑛示,杉田千恵子, 杉田 護
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] ミトコンドリアのRNA 編集に働くPPR-DYW タンパク質の同定 (2011)
  • Author(s): 内田 雅人, 大谷 祥太郎,一瀬 瑞穂, 杉田 千恵子,杉田 護
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] The complete set of PPR-DYW proteins addressing all eleven editing sites in Physcomitrella patens mitochondrial transcripts
  • Author(s): Mizuho Ichinose, Chieko Sugita, Mamoru Sugita
  • Organizer: Frontiers in Plant RNA Research 2012
  • Place of Presentation: Sapporo, Hokkaido
• [Presentation] Identification and functional analysis of a truncated PLS-type PPR protein in Physcomitrella patens
  • Author(s): Mizuho Ichinose, Hirofumi Katayama, Yamato Kato and Mamoru Sugita
  • Organizer: Frontiers in Plant RNA Research 2012
  • Place of Presentation: Sapporo, Hokkaido
• [Presentation] ヒメツリガネゴケのミトコンドリアRNA編集因子の同定
  • Author(s): 一瀬瑞穂、杉田 護
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 植物ミトコンドリアの RNA編集を統御するPPRタンパク質ネットワーク
  • Author(s): 杉田 護
  • Organizer: 平成２４年度岡山大学資源植物科学研究所共同利用・共同拠点ワークショップ「植物ミトコンドリア研究の新展開」
  • Place of Presentation: 岡山大学資源植物科学研究所、倉敷市
  • Invited
• [Presentation] 前駆体tRNAのプロセシングに働くPPRタンパク質
  • Author(s): 杉田千恵子、田中惟睦、米谷一樹、香村吉洋、松本貴嗣、吉川博文、杉田護
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学津島キャンパス
• [Presentation] ヒメツリガネゴケの新規PLS-type PPRタンパク質の同定と機能解析
  • Author(s): 一瀬瑞穂、片山博文、杉田 護
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学津島キャンパス
• [Presentation] クロララクニオン藻における葉緑体DNA配列の比較解析
  • Author(s): 鈴木重勝1、小藤累美子、平川泰久、杉田護、石田健一郎
  • Organizer: 日本藻類学会第37回山梨大会
  • Place of Presentation: 山梨大学
• [Book] Chapter 10. Dynamic features of plastid genome and its transcriptional control in plastid development.Basanti Biswal, Karin Krupinska and Udaya C. Biswal, The Advances in Photosynthesis and Respiration36"Plastid Development in Leaves during Growth and Senescence" (2013)
  • Author(s): Kanamaru, K. and Sugita, M
  • Total Pages: 685
  • Publisher: Springer-Verlag
• [Book] The Advances in Photosynthesis and Respiration series 36 on “Plastid Development in Leaves during Growth and Senescence.”, Chapter 10. Dynamic features of plastid genome and its transcriptional control in plastid development. (2013)
  • Author(s): Kanamaru, K. and Sugita, M.
  • Total Pages: 685
  • Publisher: Springer
• [Remarks] 杉田研究室ホームページ、論文リスト
  • URL: http://www.gene.nagoya-u.ac.jp/~sugita-g/ronbun.html
• [Remarks] 名古屋大学大学院・理学研究科生命理学専攻ホームページ、論文紹介
  • URL: http://www.bio.nagoya-u.ac.jp/paper/index.html
• [Remarks] 杉田研究室ホームページ、論文リスト
  • URL: http://www.gene.nagoya-u.ac.jp/~sugita-g/ronbun.html
• [Remarks] 名古屋大学大学院理学研究科生命理学専攻ホームページ、論文紹介
  • URL: http://www.bio.nagoya-u.ac.jp/paper/index.html
• [Remarks]
  • URL: http://www.gene.nagoya-u.ac.jp/~sugita-g/ronbun.html