Genomic target site recognition by SOX-partner factor complexes that underlies switching mechanisms in cell differentiation
| Project/Area Number |
23657007
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Kyoto Sangyo University (2014)
Osaka University (2011-2013) |
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Principal Investigator |
KONDOH Hisato 京都産業大学, 総合生命科学部, 教授 (70127083)
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| Co-Investigator(Kenkyū-buntansha) |
KAMACHI Yusuke 大阪大学, 生命機能研究科, 准教授 (90263334)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 発現制御 / 遺伝子 / ゲノム / 細胞分化 / 転写制御因子 / 転写因子 |
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| Outline of Final Research Achievements |
Transcription factors function as heterologous complexes, and changing their partner in the complex results in a major alteration in their regulatory targets. This mechanism is responsible for the switching of cell states during differentiation. In addition, the DNA-binding sequences of transcription factor complexes are not mere additions of sequences for individual factor binding.
In this study, we systematically analyzed in vitro and in vivo DNA-binding sequences for Sox2-partner factor complexes. (1) We collected high-affinity Sox2;Pax6 co-binding sequences in vitro using a new SELEX procedure with non-RI EMSA and characterized these sequences. (2) We improved the ChIP-seq procedure using biotinylated transcription factors and applied it to epiblast stem cells to characterize Sox2;partner (e.g., Pou5f1) co-binding sequences in vivo.
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Report
(5 results)
Research Products
(10 results)
