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Functional analysis of natural genetic variation of non-model species using model species

Research Project

Project/Area Number 23657015
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionNational Museum of Nature and Science, Tokyo

Principal Investigator

IKEDA Hajime  独立行政法人国立科学博物館, 植物研究部, 研究員 (70580405)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords分子生態 / フィトクロム / 野生変異
Research Abstract

I have focused PHYE in Cardamine nipponica that have two alleles distinguished by several amino acid replacements in wild populations. At first, I succeed cloning PHYE into a vector to induce protein synthesis in E. coli. In particular, I made chimeric constructions that exchanged some amino acid replacements between alleles. In addition, I made construct of PHYE to apply transformation of Arabidopsis thaliana. Because PHYE is a large gene (ca. 3600 bp in protein coding region), I took much time for cloning and failed to obtain sufficient data about functional differences of alleles of PHYE. Nevertheless, my progress could contribute future work to unravel functional differences of alleles found in wild populations.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (8 results)

  • [Journal Article] A multilocus sequencing approach reveals the cryptic phylogeographic history of Phyllodoce nipponica Makino (Ericaceae)2013

    • Author(s)
      Ikeda H, Setoguchi H
    • Journal Title

      Biological Journal of Linnean Society

      Volume: -

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of an alpine endemic plant at the arctic-alpine range periphery following Pleistocene climatic oscillations2012

    • Author(s)
      Ikeda, H., Carlsen, T., Fujii, N., Brochmann, C, Setoguchi, H.
    • Journal Title

      New Phytologist

      Volume: 194 Issue: 2 Pages: 583-594

    • DOI

      10.1111/j.1469-8137.2012.04061.x

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A multilocus sequencing approach reveals the cryptic phylogeographic history of Phyllodoce nipponica Makino (Ericaceae).

    • Author(s)
      Ikeda Hajime, Setoguchi Hiroaki
    • Journal Title

      Biological Journal of Linnean Society

      Volume: (出版中)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Recent colonization of a coastal plant into inland habitats at an ancient freshwater lake, Lake Biwa : multilocus sequencing and a demographic history of Lathyrus japonicus (Fabaceae)2012

    • Author(s)
      Ohtsuki Tatsuo, Ikeda Hajime, Setoguchi Hiroaki
    • Organizer
      1st Joint Congress on Evolutionary Biology
    • Place of Presentation
      Ottawa, Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular evolution of photoreceptor genes between sister species2012

    • Author(s)
      Ikeda Hajime, Gustafsson Lovisa, Brochmann Christian, Setoguchi Hiroaki
    • Organizer
      1st Joint Congress on Evolutionary Biology
    • Place of Presentation
      Ottawa, Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] 周極-高山植物における種分化と光受容体の分子進化2012

    • Author(s)
      池田啓, 岡義人, Lovisa Gustafsson, Christian Brochmann, 長谷あきら, 瀬戸口浩彰
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2012 Final Research Report
  • [Presentation] Recent colonization of a coastal plant into inland habitats at an ancient freshwater lake, Lake Biwa: multilocus sequencing and a demographic history of Lathyrus japonicus (Fabaceae)2012

    • Author(s)
      Ohtsuki, T. H. Ikeda and H. Setoguchi
    • Organizer
      1st Joint Congress on Evolutionary Biology
    • Place of Presentation
      Ottawa
    • Related Report
      2012 Annual Research Report
  • [Presentation] Molecular evolution of photoreceptor genes between sister species2012

    • Author(s)
      Ikeda, H. L. Gustafsson, C. Brochmann and H. Setoguchi
    • Organizer
      1st Joint Congress on Evolutionary Biology
    • Place of Presentation
      Ottawa
    • Related Report
      2012 Annual Research Report
  • [Presentation] 周極-高山植物における種分化と光受容体の分子進化2012

    • Author(s)
      池田啓,岡義人,Lovisa Gustafsson, Christian Brochmann,長谷あきら,瀬戸口浩彰
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ツガザクラ(Phyllodoce nipponica)における核遺伝子と葉緑体の矛盾した地理構造2012

    • Author(s)
      池田啓
    • Organizer
      日本植物分類学会第11回大会
    • Place of Presentation
      大阪
    • Related Report
      2011 Research-status Report
  • [Presentation] RhododendronのESTマーカーを用いたツツジ科イワヒゲ(Cassiope lycopodioides ) の系統地理2011

    • Author(s)
      池田啓,東広之,瀬戸口浩彰
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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