| Project/Area Number |
23657016
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ecology/Environment
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 微生物 / 生態 / 極限環境 / 好塩菌 / アーキア / 16S Rrna / G+C 含量 / 微生物分子温度計 / 16S rRNA / GC含量 / 好塩性アーキア / 環境適応 / 生育温度 / 増殖速度定数 / 16S rRNA遺伝子 / G+C含量 / 転写 / 定量RT-PCR |
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| Research Abstract |
Halophilic archaea have a few types of 16S rRNA gene on their genome. The prokaryotes, which live in high temperature, commonly have high G+C content of 16S rRNA genes, whereas the prokaryotes, which live in normal or low temperature, have low G+C content of 16S rRNA genes. In this study, we determined the growth temperatures and transcription amount of 16S rRNA genes of the halophilic archaeal strains in several temperature conditions. The low G+C content of 16S rRNAs showed significant upregulation in 25-30℃. On the other hand, high G+C content of 16S rRNAs were expressed at significantly higher levels in high temperature condition, 40-50℃.
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