| Project/Area Number |
23657025
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ecology/Environment
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
IMACHI Hiroyuki 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (20361933)
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| Co-Investigator(Renkei-kenkyūsha) |
KUBOTA Kengo 東北大学, 工学研究科, 助教 (80455807)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ファージ / 宿主域 / 16S rRNA 遺伝子 / FISH 法 / 16S rRNA遺伝子 / FISH法 |
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| Research Abstract |
We developed a novel phage-host range evaluation method using EdU (5-ethynyl-2’-deoxyuridine) labeled phages, which consists of the following four steps. (i) Fluorescently labeled phages are added to a microbial consortium, and host cells are infected and fluorescently labeled. (ii) Fluorescent cells are sorted by FACS. (iii) 16S rRNA gene sequences retrieved from sorted cells are analyzed, and specific oligonucleotide probes for FISH are designed. (iv) Cells labeled with both fluorescently labeled phage and FISH probe are identified as host cells.
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