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Engineering of photosynthetic pigment system

Research Project

Project/Area Number 23657026
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionHokkaido University

Principal Investigator

TANAKA Ayumi  北海道大学, 低温科学研究所, 教授 (10197402)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsクロロフィル / 葉緑体 / 光合成 / 機能改変 / シロイヌナズナ
Research Abstract

The light-harvesting apparatus is an interesting and usefu1 targetfor genetic modulation of photosynthesis, because it participates in the initial andessential steps of photosynthesis. We succeeded in altering the pigment arrangement inthe light-harvesting systems by introducing N-terminal truncated chlorophyllide aoxygenase, which catalyzes chlorophyll b biosynthesis, into Arabidopsis thaliana.Interestingly, thistransgenicplant showed the stay green phenotype and exhibited severephotodamage. Studies of this transgenic plant suggested the potential agriculturalapPlication of photosynthesis modification.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (60 results)

All 2013 2012 2011 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (40 results) (of which Invited: 2 results) Remarks (3 results)

  • [Journal Article] NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll-protein complexes during leaf senescence.2013

    • Author(s)
      Yamatani, H.
    • Journal Title

      Plant J.

      Volume: 74 Issue: 4 Pages: 652-662

    • DOI

      10.1111/tpj.12154

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conversion of chlorophyll b to chlorophyll a precedes magnesium dechelation for protection against necrosis in Arabidopsis.2012

    • Author(s)
      Shimoda Y, Ito H, Tanaka A
    • Journal Title

      Plant journal

      Volume: 72 Issue: 3 Pages: 501-511

    • DOI

      10.1111/j.1365-313x.2012.05095.x

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] ChlorophyllbReductase Plays an EssentialRole in Maturation and Storability of Arabidopsis thaliana Seeds2012

    • Author(s)
      Nakajima S, Ito H, Tanaka R, Tanaka A
    • Journal Title

      Physiology

      Volume: 160(1) Pages: 261-273

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chlorophyll metabolism inphotosynthetic organisms. Handbook ofPorphyrin Science, edited by Kar1 M Kadish,Kevin M Smith and Roger Guilard2012

    • Author(s)
      Tanaka R, Takabayashi A, Ito H andTanaka A
    • Journal Title

      WorldScientific, Singapore

      Volume: 20 Pages: 213-242

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] クロロフィル合成系の多様性はいかにして生まれたか2012

    • Author(s)
      伊藤寿、堕巨歩、田中亮一
    • Journal Title

      光合成研究

      Volume: 22(2) Pages: 98-105

    • URL

      http://photosyn.jp/journa1/sections/kaiho64-6

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Overproduction of chlorophyll b retardssenescence through transcriptionalre-programming in Arabidopsis2012

    • Author(s)
      Sakuraba Y, Balazadeh S, Tanaka R,Mueller-Roeber B and Tanaka A
    • Journal Title

      Plant CellPhysio1

      Volume: 53(3) Pages: 505-517

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Luminescence of singlet oxygen in photosystem II complexes isolated from cyanobacterium Synechocystis sp. PCC6803 containing monovinyl or divinyl chlorophyll a.2012

    • Author(s)
      Tomo, T., Kusakabe, H., Nagao, R., Ito, H., Tanaka, A., Akimoto, S., Mimuro, M. and Okazaki, S
    • Journal Title

      Biochim Biophys Acta.

      Volume: (in press) Issue: 8 Pages: 1299-1305

    • DOI

      10.1016/j.bbabio.2012.02.018

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Alterations in photosynthetic pigments and amino acid composition of D1 protein change energy distribution in photosystem II2012

    • Author(s)
      Makio Yokono, et al
    • Journal Title

      Biochimica et Biophysica Acta (BBA)-Bioenergetics

      Volume: (In Press)(掲載確定) Issue: 5 Pages: 754-759

    • DOI

      10.1016/j.bbabio.2012.02.009

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chlorophyll b Reductase Plays an Essential Role in Maturation and Storability of Arabidopsis thaliana Seeds.2012

    • Author(s)
      Nakajima S, Ito H, Tanaka R, Tanaka A
    • Journal Title

      Plant Physiology

      Volume: 160 Issue: 1 Pages: 261-273

    • DOI

      10.1104/pp.112.196881

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chlorophyll metabolism in photosynthetic organisms.2012

    • Author(s)
      Tanaka R, Takabayashi A, Ito H and Tanaka A
    • Journal Title

      Handbook of Porphyrin Science

      Volume: 20 Pages: 213-242

    • DOI

      10.1142/9789814335508_fmatter05

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] クロロフィル合成系の多様性はいかにして生まれたか?2012

    • Author(s)
      伊藤寿、田中歩、田中亮一
    • Journal Title

      光合成研究

      Volume: 22 Pages: 98-105

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overproduction of chlorophyll b retards senescence through transcriptional re-programming in Arabidopsis2012

    • Author(s)
      Sakuraba Y, Balazadeh S, Tanaka R, Mueller-Roeber B and Tanaka A.
    • Journal Title

      Plant Cell Physiol.

      Volume: (in press) Issue: 3 Pages: 505-517

    • DOI

      10.1093/pcp/pcs006

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evolution of a divinyl chlorophyll-based photosystem in Prochlorococcus.2011

    • Author(s)
      Ito, H. and Tanaka, A.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 108(44) Issue: 44 Pages: 18014-18019

    • DOI

      10.1073/pnas.1107590108

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Oligomeric States of Photosystems and Light-harvesting Complexes in the Chlorophyll b-less Mutant.2011

    • Author(s)
      Takabayashi, A., Kurihara, K., Kuwano, M., Kasahara, Y., Tanaka, R. and Tanaka, A.
    • Journal Title

      Plant Cell Physiol

      Volume: 52(12) Issue: 12 Pages: 2103-2114

    • DOI

      10.1093/pcp/pcr138

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Monogalactosyldiacylglycerol Synthase Found in the Green Sulfur Bacterium Chlorobaculum tepidum Reveals Important Roles for Galactolipids in Photosynthesis2011

    • Author(s)
      Shinji Masuda, et al
    • Journal Title

      The Plant Cell

      Volume: 23 Issue: 7 Pages: 2644-2658

    • DOI

      10.1105/tpc.111.085357

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of the 7-Hydroxymethyl Chlorophyll a Reductase of the Chlorophyll Cycle in Arabidopsis.2011

    • Author(s)
      Meguro, M., Ito, H., Takabayashi, A., Tanaka, R. and Tanaka, A.
    • Journal Title

      Plant Cell

      Volume: 23(9) Issue: 9 Pages: 3442-3453

    • DOI

      10.1105/tpc.111.089714

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chlorophyll cycle regulates the construction and destruction of the light-harvesting complexes.2011

    • Author(s)
      Tanaka, R. and Tanaka, A.
    • Journal Title

      Biophys Biochim Acta

      Volume: 1807 Issue: 8 Pages: 968-976

    • DOI

      10.1016/j.bbabio.2011.01.002

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Possibleinvolvement of chlorophyllase in a plantdefense system2013

    • Author(s)
      Xueyun Hu, Masanori Oshiai, TohruTsuchiya, Stefan Hortensteiner, Ayumi Tanaka, Ryouichi Tanaka
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学、岡山市
    • Year and Date
      2013-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナにおけるlight-harvesting-likeタンパク質LIL8の機能解析2013

    • Author(s)
      加藤由佳子、高林厚史、田中歩、田中亮一
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学、岡山市
    • Year and Date
      2013-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナにおけるlight-harvesting-like protein, LIL3の機能解析2013

    • Author(s)
      高橋香織、高林厚史、田中歩、田中亮一
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学、岡山市
    • Year and Date
      2013-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Chlorophyllide a oxygenase(CAO)のnegative feedback制御におけるAドメインの機能解析2013

    • Author(s)
      森恭一郎、高林厚史、田中歩
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学、岡山市
    • Year and Date
      2013-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナのクロロフィルb還元酵素の強光適応における機能解析2013

    • Author(s)
      佐藤玲惟、伊藤寿、旦虫歩
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学、岡山市
    • Year and Date
      2013-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Possible involvement of chlorophyllase in a plant defense system2013

    • Author(s)
      Xueyun Hu, Masanori Oshiai, Tohru Tsuchiya, Stefan Hortensteiner, Ayumi Tanaka, Ryouichi Tanaka
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナにおけるlight-harvesting-likeタンパク質LIL8の機能解析2013

    • Author(s)
      加藤由佳子、高林厚史、田中歩、田中亮一
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナにおけるlight- harvesting-like protein, LIL3の機能解析2013

    • Author(s)
      高橋香織、高林厚史、田中歩、田中亮一
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Chlorophyllide a oxygenase(CAO)の negative feedback 制御における A ドメインの機能解析2013

    • Author(s)
      森恭一郎、高林厚史、田中歩
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナのクロロフィルb還元酵素の強光適応における機能解析2013

    • Author(s)
      佐藤玲惟、伊藤寿、田中歩
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Degradation of chlorophyll and chlorophyll-protein complexes,Okavama Universitv2012

    • Author(s)
      Avumi Tanaka
    • Organizer
      International Symposium Structure and Dynamics of Photosynthetic Systems
    • Place of Presentation
      Okayama University,Okayama
    • Year and Date
      2012-10-22
    • Related Report
      2012 Final Research Report
  • [Presentation] light-harvesting-like proteinによるgeranylgeranyl reductaseの安定化2012

    • Author(s)
      高橋香織、高林厚史、田中歩、田中亮一
    • Organizer
      ドリコールおよびイソプレノイド研究会例会
    • Place of Presentation
      新潟大学、新潟市
    • Year and Date
      2012-09-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 緑藻の色素タンパク質複合体の生化学的手法を用いた網羅的解析2012

    • Author(s)
      功刀基、高林厚史、里虫歩
    • Organizer
      化学工学会第44回秋季大会
    • Place of Presentation
      東北大学、仙台市
    • Year and Date
      2012-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 珪藻のクロロフィル合成の解析2012

    • Author(s)
      伊藤寿、田中歩
    • Organizer
      日本藻類学会第36回大会
    • Place of Presentation
      北海道大学学術交流会館、札幌市
    • Year and Date
      2012-07-14
    • Related Report
      2012 Final Research Report
  • [Presentation] クロロフィルb合成酵素CAOのユニークな分子進化と光合成の多様化2012

    • Author(s)
      功刀基、高林厚史、田中歩
    • Organizer
      第3回日本光合成学会年会
    • Place of Presentation
      東京工業大学すずかけキャンパス、横浜市
    • Year and Date
      2012-06-01
    • Related Report
      2012 Final Research Report
  • [Presentation] Genetic engineering ofphotosystems by the modification ofchlorophyll metabolism2012

    • Author(s)
      Ayumi Tanaka
    • Organizer
      ABRC AcademiaSinica seminar
    • Place of Presentation
      Instituteof Planr and Microbial Biology, Taipei,Taiwan
    • Year and Date
      2012-04-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Chlorophyllasel isnot involved inchlorophyll breakdown duringmethyl-jasmonate-induced senescence inArabidopsis thaliana2012

    • Author(s)
      Xueyun Hu, Tohru Tsuchiya, StefanHoertensteiner, Ayumi Tanaka, RyouichiTanaka
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学、京都市
    • Year and Date
      2012-03-18
    • Related Report
      2012 Final Research Report
  • [Presentation] BN-PAGEにより精製された光合成反応中心の時間分解蛍光スペクトルの測定2012

    • Author(s)
      横野牧生,高林厚史,栗原克宜,田中歩,秋本誠志
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学、京都市
    • Year and Date
      2012-03-18
    • Related Report
      2012 Final Research Report
  • [Presentation] DivinylChlorophyllide Reductaseの性質2012

    • Author(s)
      伊藤寿、田中歩
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学、京都市
    • Year and Date
      2012-03-18
    • Related Report
      2012 Final Research Report
  • [Presentation] 新規手法による葉緑体タンパク質複合体の網羅的検出2012

    • Author(s)
      高林厚史,栗原克宜,田中亮一,田中歩
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学、京都市
    • Year and Date
      2012-03-18
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナにおける新規Light-harvesting-likeタンパク質の機能解析2012

    • Author(s)
      加藤由佳子、高林厚史、里虫歩、田中亮一
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学、京都市
    • Year and Date
      2012-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Light-harvesting chlorophyll-bindingproteinと共通するモチーフを持つタンパク質、LIL3の機能解析2012

    • Author(s)
      高橋香織、高林厚史、田中歩、田中亮一
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学、京都市
    • Year and Date
      2012-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナのクロロフィルb分解における複数経路の検証2012

    • Author(s)
      下田洋輔、伊藤寿、田中歩
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学、京都市
    • Year and Date
      2012-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Degradation of chlorophyll and chlorophyll-protein complexes2012

    • Author(s)
      Ayumi Tanaka
    • Organizer
      Okayama University International Symposium “Structure and Dynamics of Photosynthetic Systems”
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] light- harvesting-like proteinによるgeranylgeranyl reductaseの安定化2012

    • Author(s)
      高橋香織、高林厚史、田中歩、田中亮一
    • Organizer
      ドリコールおよびイソプレノイド研究会例会
    • Place of Presentation
      新潟大学(新潟市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 緑藻の色素タンパク質複合体の生化学的手法を用いた網羅的解析2012

    • Author(s)
      功刀基、高林厚史、田中歩
    • Organizer
      化学工学会第44回秋季大会
    • Place of Presentation
      東北大学(仙台市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] クロロフィルb合成酵素CAOのユニークな分子進化と光合成の多様化2012

    • Author(s)
      功刀基、高林厚史、田中歩
    • Organizer
      第3回日本光合成学会年会
    • Place of Presentation
      東京工業大学(横浜市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 珪藻のクロロフィル合成の解析2012

    • Author(s)
      伊藤寿、田中歩
    • Organizer
      日本藻類学会第36回大会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Genetic engineering of photosystems by the modification of chlorophyll metabolism2012

    • Author(s)
      Ayumi Tanaka
    • Organizer
      ABRC Academia Sinica seminar
    • Place of Presentation
      Institute of Planr and Microbial Biology (Taiwan)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Chlorophyllase1 is not involved in chlorophyll breakdown during methyl-jasmonate-induced senescence in Arabidopsis thaliana2012

    • Author(s)
      Xueyun Hu,Tohru Tsuchiya,Stefan Hoertensteiner,Ayumi Tanaka,Ryouichi Tanaka
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] BN-PAGEにより精製された光合成反応中心の時間分解蛍光スペクトルの測定2012

    • Author(s)
      横野牧生, 高林厚史, 栗原克宜, 田中歩, 秋本誠志
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Divinyl Chlorophyllide Reductaseの性質2012

    • Author(s)
      伊藤寿、田中歩
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 新規手法による葉緑体タンパク質複合体の網羅的検出2012

    • Author(s)
      高林厚史, 栗原克宜, 田中亮一, 田中歩
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] シロイヌナズナにおける新規Light-harvesting-likeタンパク質の機能解析2012

    • Author(s)
      加藤由佳子、高林厚史、田中歩、田中亮一
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Light-harvesting chlorophyll-binding proteinと共通するモチーフを持つタンパク質、LIL3の機能解析2012

    • Author(s)
      高橋香織、高林厚史、田中歩、田中亮一
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] シロイヌナズナのクロロフィルb分解における複数経路の検証2012

    • Author(s)
      下田洋輔、伊藤寿、田中歩
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 生体金属イオン生命と分子をつなぐメッセンジャー2011

    • Author(s)
      田中歩
    • Organizer
      第84回日本生化学会大会シンポジウム
    • Place of Presentation
      国立京都国際会館、京都市
    • Year and Date
      2011-09-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Evolution of chlorophyll metabolism2011

    • Author(s)
      Ayumi Tanaka
    • Organizer
      The Evolutionof Photosynthesis and Oxygenation of theEarth
    • Place of Presentation
      The Universityof New South Wales, Sydney, Australia
    • Related Report
      2012 Final Research Report
  • [Presentation] 生体金属イオン:生命と分子をつなぐメッセンジャー2011

    • Author(s)
      田中歩
    • Organizer
      第84回日本生化学会大会シンポジウム(招待講演)
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Evolution of chlorophyll metabolism.2011

    • Author(s)
      Ayumi Tanaka
    • Organizer
      The Evolution of Photosynthesis and Oxygenation of the Earth(招待講演)
    • Place of Presentation
      The University of New South Wales (Australia)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.10wtem.hokudai.ac.jp/Plantadapt/index.htm1

    • Related Report
      2012 Final Research Report
  • [Remarks] 北海道大学低温科学研究所 生物適応研究室

    • URL

      http://www.lowtem.hokudai.ac.jp/plantadapt/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.lowtem.hokudai.ac.jp/plantadapt/index.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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