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Study of molecular mechanism of asymmetry in plant stem cells

Research Project

Project/Area Number 23657027
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionHokkaido University

Principal Investigator

FUJITA Tomomichi  北海道大学, 大学院・理学研究院, 准教授 (50322631)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords植物ホルモン / 成長生理 / 全能性 / 植物 / 非対称分配 / ヒメツリガネゴケ / 転写因子 / 細胞壁タンパク質 / アブシジン酸
Research Abstract

Asymmetric cell division is important for multicellular organisms to control their developmental program. Molecular mechanisms of asymmetric cell division are still largely unknown in plants. The moss, Physcomitrella patens provides a good system for studying the mode of cell division since the whole processes are easily observable at a single cell level.We identified ten of the fluorescent protein-tagged proteins were segregated asymmetrically during cell division, and found distinct types of uneven segregation are underlying during asymmetric cell division. We also found that either under a stress condition or upon abscisic acid treatment, the apical cell changed a cell division mode from asymmetry to symmetry. Then in order to understand a molecular mechanism for how the mode of cell division can be switched, we identified several genes, and further studied their function.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (16 results)

All 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (12 results) Remarks (1 results)

  • [Journal Article] Quantitative imaging of directional transport through plasmodesmata in moss protonemata viasingle-cell photoconversion of Dendra22013

    • Author(s)
      Kitagawa, M. and Fujita, T
    • Journal Title

      Journal of Plant Research

      Volume: 126 Issue: 4 Pages: 577-585

    • DOI

      10.1007/s10265-013-0547-5

    • NAID

      10031184112

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Selaginella Genome Identifies Genetic Changes Associated with the Evolution of Vascular Plant.2011

    • Author(s)
      Banks, J.A., Nishiyama, T., Hasebe, M., Bowman, J.L., Gribskov, M., dePamphilis, C., Albert, V.A., Aono, N., Aoyama, T., Ambrose, B.A., Ashton, N.W., Axtell, M.J., Barker, E., Barker, M.S., Bennetzen, J.L., Bonawitz, N.D., Chapple, C., Cheng, C., Correa, L.G.G., Dacre, M., DeBarry, J., Dreyer, I., Elias, M., Engstrom, E.M., Estelle, M., Feng, L., Finet, C., Floyd, S.K., Frommer, W.B., F u j i t a, T ., Gramzow, L., Gutensohn, M., Harholt, J., Hattori, M., Heyl, A., Hirai, T., Hiwatashi, Y., Ishikawa, M., Iwata, M., Karol, K.G., Koehler, B., Kolukisaoglu, U., Kubo, M., Kurata, T., Lalonde, S., Li, K., Li, Y., Litt, A., Lyons, W., Manning, G., Maruyama, T., Michael, T.P., Mikami, K., Miyazaki, S., Morinaga, S., Murata, T., Mueller‐Roeber, B., Nelson, D.R., Obara, M., Oguri, Y., Olmstead, R.G., Onodera, N., Petersen, B.L., Pils, B., Prigge, M., Rensing, S.A., Riano-Pachon, D.M., Roberts, A.W., Sato, Y., Scheller, H.V., Schulz, B., Schulz, C., Shakirov, E.V.,Shibagaki, N., Shinohara, N., Shippen, D.E., Sorensen, I., Sotooka, R., Sugimoto, N., Sugita, M., Sumikawa, N., Tanurdzic, M., Theisen, G., Ulvskov, P., Wakazuki, S., Weng, J.-K., Willats, W.W.G.T., Wipf, D., Wolf, P.G., Yang, L., Zimmer, A.D., Zhu, Q., Mitros, T., Hellsten, U., Loque, D., Otillar, R., Salamov, A., Schmutz, J., Shapiro, H., Lindquist, E., Lucas, S., Rokhsar, D., Grigoriev, I.V.
    • Journal Title

      Science

      Volume: 332 Pages: 960-963

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Selaginella genome identifies genetic changes associated with the evolution of vascular plants2011

    • Author(s)
      Banks, et al
    • Journal Title

      Science

      Volume: 332 Issue: 6032 Pages: 960-963

    • DOI

      10.1126/science.1203810

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Cell wall proteins regulates asymmetric and symmetric modes of cell division2012

    • Author(s)
      Yuya Tsuchiya, Kohei Nakamura, Hiroyuki Imai, Yoichi Sakata, Ralph Quatrano, Mitsuyasu Hasebe & Tomomichi Fujita
    • Organizer
      MOSS2012 国際会議
    • Place of Presentation
      The New York Botanical Garden,Bronx, NY,米国
    • Related Report
      2012 Final Research Report
  • [Presentation] Cell wall proteins regulates asymmetric and symmetric modes of cell division2012

    • Author(s)
      Tomomichi Fujita
    • Organizer
      MOSS2012
    • Place of Presentation
      New York Botanical Garden (USA)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Plant extracellular matrix protein regulates asymmetric and symmetric modes of cell divisio2011

    • Author(s)
      藤田知道
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒメツリガネゴケにおけるCDKAの機能解析

    • Author(s)
      石橋充浩、巻口勇馬、野田なつみ、日渡祐二、石川雅樹、鈴木穣、菅野純夫、長谷部光泰、藤田知道
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒメツリガネゴケ原糸体の発生過程における原形質連絡のサイズ排除限界の変化

    • Author(s)
      Munenori Kitagawa, Makoto Terauchi, Tomoaki Nishiyama, Miharu Ayabe, Taizo Motomura, Tomomichi Fujita
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒメツリカネコケ原糸体における分子ふるい効果

    • Author(s)
      綾部美晴,北川宗典,藤田知道
    • Organizer
      日本植物学会第76会大会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒメツリガネゴケ原糸体の発生過程における原形質連絡のサイズ排除限界の変化

    • Author(s)
      Munenori Kitagawa, Makoto Terauchi, Tomoaki Nishiyama, Miharu Ayabe, Taizo Motomura, Tomomichi Fujita
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都産業大
    • Related Report
      2012 Final Research Report
  • [Presentation] アブシジン酸により誘導される細胞分裂と細胞分化に関わる新奇因子の同定と解析

    • Author(s)
      土屋祐弥、今井裕之、中村康平、坂田洋一、Ralph Quatrano,長谷部光泰、藤田知道
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都産業大
    • Related Report
      2012 Final Research Report
  • [Presentation] Recapitulation of parasitic nematode secretion activity in plant cells

    • Author(s)
      中野智陽、藤田知道、大崎満、後藤デレック
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都産業大
    • Related Report
      2012 Final Research Report
  • [Presentation] Plant extracellular matrix protein regulates asymmetric and symmetric modes of cell division

    • Author(s)
      土屋祐弥,中村康平,今井裕之,坂田洋一,Ralph Quatrano,長谷部光泰,藤田知道
    • Organizer
      第34回日本分子生物学会年
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] The change of size exclusion limit of plasmodesmata in the filamentous tissue, protonemata of Physcomitrella patens

    • Author(s)
      Munenori Kitagawa, Makoto Terauchi, Tomoaki Nishiyama, Taizo Motomura, Tomomichi Fujita
    • Organizer
      MOSS2011 国際コケ植物会議
    • Place of Presentation
      Leistungszentrum Herzogenhorn,Freiburg, Germany
    • Related Report
      2012 Final Research Report
  • [Presentation] Imaging analysis of intercellular communication of macromolecules through plasmodesmata in plants

    • Author(s)
      Munenori Kitagawa, Tomoaki Nishiyama, Tomomichi Fujita
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道大学
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.sci.hokudai.ac.jp/~tfujita/Fujita/welcome.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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