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Establishment of an extracellular secretion pathway of flavonoids via a vesicle transport

Research Project

Project/Area Number 23657028
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionTohoku University

Principal Investigator

NAKAYAMA Toru  東北大学, 工学(系)研究科(研究院), 教授 (80268523)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsイソフラボン / ダイズ / 小胞 / 分泌 / 輸送 / 培養細胞 / 国際情報交換 / 国際情報交流
Research Abstract

In this study, changes in the transcription levels of a gene coding for an isoflavone specific beta-glucosidase of soybean, GmICHG, in the individual organs of soybean seedlings (cv. Enrei) in response to microbial infection and abiotic stresses were analyzed. GmICHG was originally expressed in abundance only in the roots and at low levels only in the other organs. The transcription of GmICHG in the roots and other organs was suppressed upon infection of the roots by Phytophthora sojae. Upon wounding of the cotyledon, a transient long-distance up-regulation of GmICHG transcription in the roots was observed. Cultured cells of soybean (cv. Enrei) were established. The cells contained isoflavones whose composition was very similar to that of the seedling roots. A method for transforming the cultured cells by Agrobacterium method and an imaging system of intracellular flavonoids were also established.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (5 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] Transcription analyses of GmICHG, a gene coding for a b-glucosidase that catalyzes the specific hydrolysis of isoflavone conjugates in Glycine max (L.) Merr2013

    • Author(s)
      DongChan Yoo
    • Journal Title

      Plant Science

      Volume: 208 Pages: 10-19

    • DOI

      10.1016/j.plantsci.2013.03.006

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Purification, gene cloning, and biochemical characterization of a β-glucosidase capable of hydrolyzing sesaminol triglucoside from Paenibacillus sp. KB05492013

    • Author(s)
      Nair, A., Kuwahara, A. Nagase, A. Yamaguchi, H. Yamazaki, T., Hosoya, M., Omura, A., Kiyomoto, K., Yamaguchi, M., Shimoyama, T., Takahashi, S., and Nakayama, T.
    • Journal Title

      PLOS ONE

      Volume: 8 Issue: 4 Pages: e60538-e60538

    • DOI

      10.1371/journal.pone.0060538

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene cloning and biochemical ...2012

    • Author(s)
      Yamaguchi, H. et al.
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 111 Pages: 522-527

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quantitative analyses of the behavior of ...2012

    • Author(s)
      Suematsu, T. et al.
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 印刷中

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Catalytic removal of acetaldehyde in saliva ...2012

    • Author(s)
      Yamaguchi, H. et al.
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 印刷中

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 平行進化する基質特異性2012

    • Author(s)
      小埜栄一郎,堀川 学,中山 亨
    • Journal Title

      化学と生物

      Volume: 50 Pages: 16-22

    • NAID

      10030818742

    • Related Report
      2011 Research-status Report
  • [Journal Article] 植物フラボノイドグリコシルトランスフェラーゼ:分子系統学と特異性発現機構2012

    • Author(s)
      中山 亨,堀川 学,小埜栄一郎
    • Journal Title

      酵素工学ニュース

      Volume: 印刷中

    • Related Report
      2011 Research-status Report
  • [Presentation] ダイズにおけるイソフラボン主骨格生合成酵素群のタンパク質相互作用解析2014

    • Author(s)
      和氣駿之, 本橋令子, 高橋征司, 中山亨
    • Organizer
      日本農芸化学会2014年度(東京)大会
    • Place of Presentation
      明治大学生田キャンパス
    • Year and Date
      2014-03-30
    • Related Report
      2013 Final Research Report
  • [Presentation] ダイズにおけるイソフラボン主骨格生合成酵素群の タンパク質相互作用解析2014

    • Author(s)
      和氣駿之 本橋令子 高橋征司 中山 亨
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] フラボノイドアグリコンの合成・修飾・脱修飾を介する植物の環境応答制御2013

    • Author(s)
      中山 亨
    • Organizer
      日本農芸化学会2013年度(仙台)大会 シンポジウム4SY13
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-27
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ダイズのイソフラボングルコシルトランスフェラーゼの触媒機能とタンパク質間相互作用2012

    • Author(s)
      中山 亨
    • Organizer
      日本農芸化学会大会2012(招待講演)
    • Place of Presentation
      京都女子大学
    • Related Report
      2011 Research-status Report
  • [Presentation] フラボノイドアグリコンの合成・修飾・脱修飾を介する植物の環境応答制御

    • Author(s)
      中山 亨
    • Organizer
      日本農芸化学会2013年度(仙台)大会 シンポジウム4SY13
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 植物の分子育種学2011

    • Author(s)
      鈴木正彦 編著 中山 亨他22名 共著
    • Publisher
      講談社
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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