Biochemical properties and physiological role of novel proteins involved in regulation of phosphatidylinositol phosphates.

• Project/Area Number: 23657031
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: MAESHIMA Masayoshi 名古屋大学, 生命農学研究科, 教授 (80181577)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 植物分子機能 / 情報伝達 / カルシウム / 細胞膜 / 情報変換 / ホスファチジルイノシトールリン酸 / カルモジュリン / 植物細胞 / タンパク質

Research Abstract

Plasma membrane-associated Ca2+-binding proteins (PCaPs) of Arabidopsis thaliana is a novel-type proteins that bind to the calmodulin (CaM)/Ca complex and phosphatidylinositol phosphates (PIPs) at their N-terminal parts. PCaPs are myristoylated and stably associated with the plasma membrane. PCaPs bind PIPs at resting state of cells and CaM/Ca when the cytoplasolic Ca2+level is increased. Thus, it is estimated that PCaPs function as transducers from Ca-signaling to PIP-signaling. During the present study, we demonstrated that PCaP1 is involved in closure of stomata in leaves and that PCaP2 regulates root hair tip growth via processing Ca2+and PtdInsP signals on the plasma membrane.

Research Products

• [Journal Article] The ABCA9 transporter facilitates seeds storage lipid synthesis at the endoplasmic reticulum (2013)
  • Author(s): Kim, S., Yamaoka, Y., Ono, H., Kim, H., Shim, D., Maeshima, M., Martinoia, E., Cahoon, E.B., Nishida, I., Lee, Y
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A Volume: 110 Pages: 773-778
  • Peer Reviewed
• [Journal Article] Arabidopsis TWISTED DWARF1 functionally interacts with auxin experter ABCB1 on the root plasma membrane (2013)
  • Author(s): Wang, B., Bailly, A., Zwiewka, M., Henrichs, S., Azzarello, E., Mancuso, S., Maeshima, M., Friml, J., Schulz, A., Geisler, M
  • Journal Title: Plant Cell Volume: 25 Pages: 202-214
  • Peer Reviewed
• [Journal Article] The Ca2+-binding protein PCaP2 located on the plasma membrane is involved in root hair development as a possible signal transducer (2013)
  • Author(s): Kato, M., et. al.
  • Journal Title: Plant Journal Volume: (印刷中) Issue: 4 Pages: 690-700
  • DOI: 10.1111/tpj.12155
  • Peer Reviewed
• [Journal Article] Zinc-binding and structural properties of the histidine-rich loop of Arabidopsis thaliana vacuolar membrane zinc transporter MTP1 (2013)
  • Author(s): Tanaka, N., Kawachi, M., Fujiwara, T.,Maeshima, M.
  • Journal Title: FEBS Open Bio
  • Peer Reviewed
• [Journal Article] The ABCA9 transporter facilitates seeds storage lipid synthesis at the endoplasmic reticulum. (2013)
  • Author(s): Kim, S., Yamaoka, Y., Ono, H., Kim, H., Shim, D., Maeshima, M., Martinoia, E., Cahoon, E.B., Nishida, I., Lee, Y.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 110 Issue: 2 Pages: 773-778
  • DOI: 10.1073/pnas.1214159110
  • Peer Reviewed
• [Journal Article] Arabidopsis TWISTED DWARF1 functionally interacts with auxin experter ABCB1 on the root plasma membrane. (2013)
  • Author(s): Wang, B., Bailly, A., Zwiewka, M., Henrichs, S., Azzarello, E., Mancuso, S., Maeshima, M., Friml, J., Schulz, A., Geisler, M.
  • Journal Title: Plant Cell Volume: 25 Issue: 1 Pages: 202-214
  • DOI: 10.1105/tpc.112.105999
  • Peer Reviewed
• [Journal Article] Zinc-binding and structural properties of the histidine-rich loop of Arabidopsis thaliana vacuolar membrane zinc transporter MTP1. (2013)
  • Author(s): Tanaka, N., Kawachi, M., Fujiwara, T., Maeshima, M.
  • Journal Title: FEBS Open Bio Volume: In press
  • Peer Reviewed
• [Journal Article] Regulation of pyrophosphate levels by the H+-PPase is central for proper resumption of early plant development (2012)
  • Author(s): Ferjani, A., Segami, S., Horiguchi, G., Sakata, A., Maeshima, M., Tsukaya, H
  • Journal Title: Plant Signaling & Behavior Volume: 7 Pages: 38-42
  • Peer Reviewed
• [Journal Article] Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement (2012)
  • Author(s): Vijayapalani, P., Maeshima, M.,Nagasaki-Takekuchi, N., Miller, W.A
  • Journal Title: PLoS Pathogens Volume: 8(4)
  • Peer Reviewed
• [Journal Article] Amino acid screening based on structural modeling identifies critical residues for the function, ion selectivity and structure of Arabidopsis MTP1 (2012)
  • Author(s): Kawachi, M., Kobae, Y., Kogawa, S., Mimura, T., Kramer, U., Maeshima, M
  • Journal Title: FEBS J. Volume: 279 Pages: 2339-2356
  • Peer Reviewed
• [Journal Article] Stimulation of root growth induced by aluminum in Quercus serrata Thunb. is related to activity of nitrate reductase and maintenance of IAA concentration in roots (2012)
  • Author(s): Tomioka, R., Takenaka, C., Maeshima, M., and Tezuka, T., Kojima, M., Sakakibara, H
  • Journal Title: American J. Plant Sciences Volume: 3 Pages: 1619-1624
  • Peer Reviewed
• [Journal Article] Regulation of pyrophosphate levels by the H+-PPase is central for proper resumption of early plant development. (2012)
  • Author(s): Ferjani, A., Segami, S., Horiguchi, G., Sakata, A., Maeshima, M., Tsukaya, H.
  • Journal Title: Plant Signaling & Behavior, Volume: 7 Pages: 38-42
  • Peer Reviewed
• [Journal Article] Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement (2012)
  • Author(s): Vijayapalani, P., Maeshima, M., Nagasaki-Takekuchi, N., Miller, W.A.
  • Journal Title: PLoS Pathogens Volume: 8 Issue: 4 Pages: e1002639-e1002639
  • DOI: 10.1371/journal.ppat.1002639
  • Peer Reviewed
• [Journal Article] Amino acid screening based on structural modeling identifies critical residues for function, ion selectivity and structure of Arabidopsis MTP1 (2012)
  • Author(s): Kawachi, M., et al
  • Journal Title: FEBS Journal Volume: (掲載確定) Issue: 13 Pages: 2339-2356
  • DOI: 10.1111/j.1742-4658.2012.08613.x
  • Peer Reviewed
• [Journal Article] Regulation of pyrophosphate levels by the H+-PPase is central for proper resumption of early plant development. (2012)
  • Author(s): Ferjani, A., Segami, S., Horiguchi, G., Sakata, A., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Signaling & Behavior Volume: 7 Pages: 38-42
  • Peer Reviewed
• [Journal Article] Amino acid screening based on structural modeling identifies critical residues for function, ion selectivity and structure of Arabidopsis MTP1. (2012)
  • Author(s): Kawachi, M., Kobae, Y., Kogawa, S., Mimura, T., Krämer, U., and Maeshima, M.
  • Journal Title: FEBS Journal Volume: In press
  • Peer Reviewed
• [Journal Article] The iTRAQ analysis reveals mechanisms of growth defects due to excess zinc in Arabidopsis thaliana (2011)
  • Author(s): Fukao, Y., Ferjani, A., Tomioka, R., Nagasaki, N., Kurata, R., Nishimori, Y., Fujiwara, M., Maeshima, M
  • Journal Title: Plant Physiol. Volume: 155 Pages: 1893-1907
  • Peer Reviewed
• [Journal Article] Chilling-induced changes of vacuolar proton pumps of hypocotyls from Vigna unguiculata (2011)
  • Author(s): Oliveira, L.M.N., Sobreira, A.C., Sobreira, A.C.M., Costa, J.H., Otoch, M.L.O., Maeshima, M., Fernandes de Melo, D
  • Journal Title: Plant Growth Regulation Volume: 64 Pages: 211-219
  • Peer Reviewed
• [Journal Article] Keep an eye on PPi: The vacuolar-type H+-pyrophosphatase regulates postgerminative development in Arabidopsis (2011)
  • Author(s): Ferjani, A., Segami, S., Horiguchi, G., Muto, Y., Maeshima, M., Tsukaya, H
  • Journal Title: Plant Cell Volume: 23 Pages: 2895-2908
  • Peer Reviewed
• [Journal Article] The small GTPase Rab5a is essential for intracellular transport of proglutelin from Golgi apparatus to the protein storage vacuole and endosomal membrane organization in developing rice endosperm (2011)
  • Author(s): Fukuda, M., Satoh-Cruz, M., Wen, L., Crofts, A.J., Sugino, A., Washida, H., Okita, T.W., Ogawa, M., Y. Kawagoe, Y., Maeshima, M., Kumamaru, T
  • Journal Title: Plant Physiol Volume: 157 Pages: 632-644
  • Peer Reviewed
• [Journal Article] The iTRAQ analysis reveals mechanisms of growth defects due to excess zinc in Arabidopsis thaliana (2011)
  • Author(s): Fukao Y, Ferjani A, Tomioka R, Nagasaki N, Kurata R, Nishimori Y, Fujiwara M, Maeshima M
  • Journal Title: Plant Physiology Volume: 155 Issue: 4 Pages: 1893-1907
  • DOI: 10.1104/pp.110.169730
  • Peer Reviewed
• [Journal Article] Chilling-induced changes of vacuolar proton pumps of hypocotyls from Vigna unguiculata (2011)
  • Author(s): Oliveira LMN, Sobreira AC, Sobreira ACM, Costa JH, Otoch MLO, Maeshima M, Fernandes de Melo, D
  • Journal Title: Plant Growth Regulation Volume: 64 Issue: 3 Pages: 211-219
  • DOI: 10.1007/s10725-010-9559-5
  • Peer Reviewed
• [Journal Article] Keep an Eye on PPi : The Vacuolar-Type H^+-Pyrophosphatase Regulates Postgerminative Development in Arabidopsis (2011)
  • Author(s): Ferjani, A.
  • Journal Title: THE PLANT CELL Volume: 23(8) Issue: 8 Pages: 2895-2908
  • DOI: 10.1105/tpc.111.085415
  • Peer Reviewed
• [Presentation] Dimerization of GFPs fused to H+-pyrophosphatase influences the morphology and dynamics of vacuoles (2013)
  • Author(s): Segami, S., Makino, S., Miyake, A., Asaoka, M., Maeshima, M
  • Organizer: International Workshop on Plant Membrane Biology
  • Place of Presentation: Okayama
• [Presentation] シロイヌナズナ新奇 Ca2+結合タンパク質 PCaP2 は Ca2+シグナルをホスファチジルイノシトールシグナルに変換し、根毛伸長に寄与する (2013)
  • Author(s): 加藤真理子、青山卓史、前島正義
  • Organizer: 日本植物生理学会 2013 年度年会
  • Place of Presentation: 岡山
• [Presentation] シロイヌナズナ無根毛変異株を用いたリン酸欠乏時における根毛の役割の解析 (2013)
  • Author(s): 田中奈月、加藤-藤原真理子、青山卓史、富岡利恵、倉田理恵、深尾陽一朗、前島正義
  • Organizer: 日本植物生理学会 2013 年度年会
  • Place of Presentation: 岡山
• [Presentation] シロイヌナズナ新奇Ca2+結合タンパク質PCaP2はCa2+シグナルをホスファチジルイノシトールシグナルに変換し、根毛伸長に寄与する. (2013)
  • Author(s): 加藤真理子、青山卓史、前島正義
  • Organizer: 日本植物生理学会2013年度年会
  • Place of Presentation: 岡山大学
• [Presentation] シロイヌナズナ無根毛変異株を用いたリン酸欠乏時における根毛の役割の解析. (2013)
  • Author(s): 田中奈月、加藤–藤原真理子、青山卓史、富岡利恵、倉田理恵、深尾陽一朗、前島正義
  • Organizer: 日本植物生理学会2013年度年会
  • Place of Presentation: 岡山大学
• [Presentation] 細胞膜局在型新規Ca結合タンパク質PCaP1の構造とリガンド結合の解析 (2012)
  • Author(s): 相羽孝亮、長崎-武内菜穂子、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 液胞膜可視化で見えること:シロイヌナズナの根端細胞は高温下で中心液胞化が促進される (2012)
  • Author(s): 三宅愛、瀬上紹嗣、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 新規情報変換分子 PCaP1 の生理機能とくに気孔閉口における役割の解明 (2012)
  • Author(s): 三輪智佳、永田千咲子、林優紀、木下俊則、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 液胞膜亜鉛輸送体MTP1のイオン選択性と輸送機能を支えるHis領域の役割 (2012)
  • Author(s): 田中奈月、河内美樹、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] シロイヌナズナ新奇 Ca2+結合タンパク質 PCaP2 は Ca2+シグナルをホスファチジルイノシトールシグナルに変換し根毛伸長に寄与する (2012)
  • Author(s): 加藤真理子、青山卓史、前島正義
  • Organizer: 日本分子生物学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] シロイヌナズナ無根毛変異株の養分吸収と環境耐性に関する分子生理学的研究 (2012)
  • Author(s): 田中奈月、加藤真理子、青山卓史、富岡利恵、倉田理恵、深尾陽一朗、前島正義
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学
• [Presentation] Molecular diversity of zinc transporters in plants. Symposium on Transport of Functional Small Molecules in Plants (2012)
  • Author(s): Kawachi M., Kogawa, S., Fukao, Y., Kraemer U., Tanaka N., Maeshima M
  • Organizer: Annual Meting of Japan Society for Bioscience, Biotechnology, and Agrochemsitry
  • Place of Presentation: Koyoto
• [Presentation] 根毛をもたないシロイヌナズナ変異株NR23の分子生理学的な特徴 (2012)
  • Author(s): 田中奈月、加藤真理子、青山卓史、富岡利恵、前島正義
  • Organizer: 日本植物生理学会2012年度年会
  • Place of Presentation: 京都
• [Presentation] 細胞膜局在型新規Ca結合タンパク質PCaP1の構造とリガンド結合の解析. (2012)
  • Author(s): 相羽孝亮、長崎-武内菜穂子、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 新規情報変換分子PCaP1の生理機能とくに気孔閉口における役割の解明. (2012)
  • Author(s): 三輪智佳、永田千咲子、林優紀、木下俊則、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] シロイヌナズナ新奇Ca2+結合タンパク質PCaP2はCa2+シグナルをホスファチジルイノシトールシグナルに変換し根毛伸長に寄与する. (2012)
  • Author(s): 加藤真理子、青山卓史、前島正義
  • Organizer: 日本分子生物学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 毛をもたないシロイヌナズナ変異株NR23の分子生理学的な特徴 (2012)
  • Author(s): 田中奈月、加藤真理子、青山卓史、富岡利恵、前島正義：
  • Organizer: 日本植物生理学会2012年度年会
  • Place of Presentation: 京都産業大学
• [Presentation] 気孔開閉に関わる新規情報伝達タンパク質PCaP1の分子特性と高CO2応答 (2011)
  • Author(s): 永田千咲子、長崎-武内菜穂子、三輪智佳、木下俊則、前島正義
  • Organizer: 日本生化学会大会シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2011-09-21
• [Presentation] 細胞膜型カルシウム結合タンパク質 PCaP1 により発現抑制される SKS6 の分子生物学 (2011)
  • Author(s): 三輪智佳、大内雄矢、水藤百江、前島正義
  • Organizer: 日本生化学会中部支部例会
  • Place of Presentation: 静岡県立大学
  • Year and Date: 2011-05-28
• [Presentation] 細胞膜結合性新規カルモジュリン結合タンパク質 PCaP1 の分子生物学的解析 (2011)
  • Author(s): 秋田瑞穂、長崎-武内菜穂子、大内雄矢、水藤百江、前島正義
  • Organizer: 日本生化学会中部支部例会
  • Place of Presentation: 静岡県立大学
  • Year and Date: 2011-05-28
• [Presentation] 新規カルモジュリン結合タンパク質PCaP1の分子機能の解明 (2011)
  • Author(s): 秋田瑞穂、長崎菜穂子、大内雄矢、水藤百江、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] The JSPS Colloquium in Stavanger on "Abiotic stress from genes to biosphere." (2011)
  • Author(s): Maeshima, M., Tshuchira, A., Segami, S
  • Place of Presentation: Stavanger, Norway
• [Presentation] A calcium-binding proteins, CCaP1 and CCaP2, are induced by a long-period dark in Arabidopsis thaliana (2011)
  • Author(s): Ouchi, Y., Nagatani, A. and Maeshima, M
  • Organizer: The Scandinavian Plant Physiology Society Meeting 2011
  • Place of Presentation: Stavanger, Norway
• [Presentation] 細胞膜結合性新規カルモジュリン結合タンパク質PCaP1の分子生物学的解析. (2011)
  • Author(s): 秋田瑞穂、長崎-武内菜穂子、大内雄矢、水藤百江、前島正義
  • Organizer: 日本生化学会中部支部例会
  • Place of Presentation: 静岡県立大学
• [Presentation] 気孔開閉に関わる新規情報伝達タンパク質PCaP1の分子特性と高CO2応答 (2011)
  • Author(s): 永田千咲子、長崎-武内菜穂子、三輪智佳、木下俊則、前島正義
  • Organizer: 日本生化学会大会シンポジウム(招待講演)
  • Place of Presentation: 京都国際会館
• [Book] Regulation of PPi levels through vacuolar membrane H+-pyrophosphatase (2013)
  • Author(s): Ferjani, A., Segami, S., Asaoka, M., Maeshima, M
  • Publisher: Luettge, Springer
• [Book] Progress in Botany, Vol. 75, (2013)
  • Author(s): Ferjani, A., Segami, S., Asaoka, M., Maeshima, M.
  • Publisher: Springer.
• [Book] The role of membrane transport in the detoxification and accumulation of zinc in plants. In A Soil Biology Series: Detoxification of Heavy Metals (2011)
  • Author(s): Kawachi, M., Kobae, Y., Tomioka, R., Maeshima, M
  • Publisher: Springer-Verlag
• [Book] Application of radioisotope in biochemical analysis: Metal binding proteins and metal transporters. InRadioisotopes - Applications in Bio-Medical Science (2011)
  • Author(s): Kawachi, M., Nagasaki-Takeuchi, N., Kato, M., Maeshima, M.
• [Book] A Soil Biology Series: Detoxification of Heavy Metals. (The role of membrane transport in the detoxification and accumulation of zinc in plants.) (2011)
  • Author(s): Kawachi, M., Kobae, Y., Tomioka, R., and Maeshima, M.
  • Total Pages: 448
  • Publisher: Springer-Verlag.
• [Book] Radioisotopes - Applications in Bio-Medical Science (Application of radioisotope in biochemical analysis: Metal binding proteins and metal transporters) (2011)
  • Author(s): Kawachi, M., Nagasaki-Takeuc hi, N., Kato, M., and Maeshima, M.
  • Total Pages: 320
  • Publisher: In Tech