Reconstruction of circadian clocks in plant clock-less culture cells

• Project/Area Number: 23657033
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: OYAMA Tokitaka 京都大学, 大学院・理学研究科, 准教授 (30324396)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 環境応答 / 概日時計 / 培養細胞 / 概日リズム / 植物 / 分化 / カルス

Research Abstract

We established a strain of duckweed culture cells that lacked the circadian rhythmicity. Culture cells are a model for the cells with a number of perturbations of circadian oscillation that could be generated through the processes of differentiation/de-differentiation and cell proliferation. We tried reconstruction of the clock system in the clock-less culture cells by gene modification.

Research Products

• [Journal Article] Self-arrangement of cellular circadian rhythms through phase-resetting in plant roots (2012)
  • Author(s): Fukuda H, Ukai K, Oyama T
  • Journal Title: Phys. Rev. E Volume: vol. 86 Pages: 41817-41817
  • Peer Reviewed
• [Presentation] 高等植物の概日リズム特性 (2012)
  • Author(s): 小山時隆
  • Organizer: 第19回日本時間生物学会学術大会
  • Place of Presentation: 北海道大学学術交流会館(北海道)
  • Year and Date: 2012-09-15
• [Presentation] Characterization of cellular circadian oscillators in plants (2012)
  • Author(s): 小山時隆
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-18
• [Presentation] Characterization of cellular circadian oscillators in plants (2012)
  • Author(s): 小山時隆
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学（京都）
• [Presentation] Gene introduction methods for the analysis of biological rhythms in duckweeds (2011)
  • Author(s): 小山時隆
  • Organizer: The 1st International Conference of Duckweed Research & Applications
  • Place of Presentation: Wangjiang Hotel(中国成都市)
  • Year and Date: 2011-10-08
• [Presentation] 高等植物の概日リズム特性
  • Author(s): 小山時隆
  • Organizer: 第１９回日本時間生物学会学術大会
  • Place of Presentation: 北海道大学学術交流会館（北海道）
• [Remarks]
  • URL: http://cosmos.bot.kyoto-u.ac.jp/clock/index.html