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Does CO2regulate the intracellular chloroplast positioning?

Research Project

Project/Area Number 23657035
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionOsaka University

Principal Investigator

TAKAGI Shingo  大阪大学, 大学院・理学研究科, 准教授 (10192626)

Research Collaborator ISHIDA Yasuhiro  大阪大学, 大学院・理学研究科, 大学院生
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords細胞間隙二酸化炭素濃度 / シロイヌナズナ / 二酸化炭素 / 背腹性 / 葉肉細胞 / 葉緑体
Research Abstract

:Chloroplasts absorb the photosynthetic substrate CO2from the intercellular air spaces. Since chloroplasts in mesophyll cells are adjacent to the cell wall along the intercellular air spaces but not the neighboring cells, it has been hypothesized that chloroplastsare located at the cell wall containing higher CO2, although without any experimental verification. By embedding mesophyll protoplasts in a gel, we obtained the results strongly supporting the hypothesis. Further, by regulating the CO2concentration in the intercellular air spaces of each leaf, we proposed that the final destination of chloroplasts is determined through cross talk between the light intensity and the local CO2concentration in the cell wall.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (33 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 3 results) Presentation (22 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] LITTLE NUCLEI 1 and 4 regulate nuclear morphology in Arabidopsis thaliana2013

    • Author(s)
      Y. Sakamoto, S. Takagi
    • Journal Title

      Plant and Cell Physiology

      Volume: 54 Issue: 4 Pages: 622-633

    • DOI

      10.1093/pcp/pct031

    • NAID

      10031163853

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] ストロボスコープ型遠心顕微鏡による運動現象の解析2012

    • Author(s)
      高木慎吾
    • Journal Title

      Plant Morphology

      Volume: 24 Pages: 5-11

    • NAID

      130004726669

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Actin-dependent chloroplast anchoring is regulated by Ca2+-calmodulin in spinach mesophyll cells2011

    • Author(s)
      H. Takamatsu, S. Takagi
    • Journal Title

      Plant and Cell Physiology

      Volume: 52 Issue: 11 Pages: 1973-1982

    • DOI

      10.1093/pcp/pcr130

    • NAID

      10030305534

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic behavior of double-membrane-bounded organelles in plant cells2011

    • Author(s)
      Takagi, S., Islam, M.S., Iwabuchi, K.
    • Journal Title

      International Review of Cell and Molecular Biology

      Volume: VOL.286 Pages: 181-222

    • DOI

      10.1016/b978-0-12-385859-7.00004-5

    • Related Report
      2012 Final Research Report
  • [Journal Article] Possible involvement of phytochromes in regulation of cytoplasmic motility in Arabidopsis thalianaleaf epidermal cells2011

    • Author(s)
      N. Sakurai-Ozato, S. Takagi
    • Journal Title

      InTreatise on Photophysiology (eds. C. Haldar and P. Basu)

      Pages: 55-65

    • Related Report
      2012 Final Research Report
  • [Journal Article] Light-induced mitochondrial redistribution in Arabidopsis thalianapalisade cells2011

    • Author(s)
      M.S. Islam, Y. Niwa, S. Takagi
    • Journal Title

      InTreatise on Photophysiology (eds. C. Haldar and P. Basu)

      Pages: 167-171

    • Related Report
      2012 Final Research Report
  • [Journal Article] 植物オルガネラは忙しい2011

    • Author(s)
      高木慎吾
    • Journal Title

      生産と技術

      Volume: 63 Pages: 74-76

    • NAID

      40018919518

    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Journal Article] Actin-dependent chloroplast anchoring is regulated by Ca2+-calmodulin in spinach mesophyll cells.2011

    • Author(s)
      Takamatsu H., Takagi S.
    • Journal Title

      Plant and Cell Physiology

      Volume: 52 Pages: 1309-1319

    • NAID

      10030305534

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 植物ビリンは葉緑体アンカーに関与するか2013

    • Author(s)
      高木慎吾
    • Organizer
      第15回植物オルガネラワークショップ
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-20
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナ葉肉細胞における核光定位運動への葉緑体の関与2013

    • Author(s)
      田中怜、岩渕功誠、西村いくこ、高木慎吾
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Final Research Report
  • [Presentation] Phototropin2- dependent co-localization of mitochondria with chloroplasts in Arabidopsis thalianamesophyll cells2012

    • Author(s)
      M.S. Islam, S. Takagi
    • Organizer
      平成24年度近畿植物学会
    • Place of Presentation
      奈良
    • Year and Date
      2012-11-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of LINCs involved in the maintenance of nuclear morphology in Arabidopsis thaliana2012

    • Author(s)
      Y. Sakamoto, S. Takagi
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナにおける細胞核形態を維持するLINCの解析2012

    • Author(s)
      坂本勇貴、高木慎吾
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Related Report
      2012 Final Research Report
  • [Presentation] Light-dependent nuclear positioning serves as a UV-protection system in plants2012

    • Author(s)
      Iwabuchi K., Hidema J., Tamura K., Takagi S., Hara-Nishimura I.
    • Organizer
      The 1st international symposium on plant environmental sensing
    • Place of Presentation
      Nara, Japan
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] Light-dependent nuclear positioning reduces UV-induced DNA damage in plants2012

    • Author(s)
      Iwabuchi K., Hidema J., Tamura K., Takagi S., Hara-Nishimura I
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] シロイヌナズナにおける細胞核形態を維持する因子の解析2012

    • Author(s)
      坂本勇貴、高木慎吾
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] Roles of phototropins and myosins in the light-induced redistribution of mitochondria and chloroplasts in leaf palisade cells of Arabidopsis thaliana.International Symposium2011

    • Author(s)
      Nguyen V.T., Islam M.S., Takagi S
    • Organizer
      Strategies of Plants against Global Environmental Change
    • Place of Presentation
      Kurashiki, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] イメージングによる細胞内運動の解析2011

    • Author(s)
      高木慎吾
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] Photoregulation of mitochondria/chloroplasts co-localization in Arabidopsis thalianamesophyll cells2011

    • Author(s)
      Islam M.S., Takagi S
    • Organizer
      The 5th Asia Oceania Conference on Photobiology
    • Place of Presentation
      Nara, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Regulation of chloroplast positioning by light and CO2.2011

    • Author(s)
      Ishida Y., Takagi S
    • Organizer
      The 5th Asia Oceania Conference on Photobiology
    • Place of Presentation
      Nara, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] ホウレンソウ葉肉細胞のアクチン細胞骨格と葉緑体アンカーはCa2+-カルモデュリンによって制御される2011

    • Author(s)
      高松秀安、高木慎吾
    • Organizer
      2011年植物科学談話会
    • Place of Presentation
      大阪市立大学付属植物園
    • Related Report
      2011 Research-status Report
  • [Presentation] Photoregulation of mitochondria/chloroplasts co-localization in Arabidopsis thaliana mesophyll cells2011

    • Author(s)
      Islam M.S., Takagi S.
    • Organizer
      The 5th Asia Oceania Conference on Photobiology
    • Place of Presentation
      Nara, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Regulation of chloroplast positioning by light and CO22011

    • Author(s)
      Ishida Y., Takagi S.
    • Organizer
      The 5th Asia Oceania Conference on Photobiology
    • Place of Presentation
      Nara, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Roles of phototropins and myosins in the light-induced redistribution of mitochondria and chloroplasts in leaf palisade cells of Arabidopsis thaliana2011

    • Author(s)
      Nguyen V.T., Islam M.S., Takagi S.
    • Organizer
      International Symposium 'Strategies of Plants against Global Environmental Change'
    • Place of Presentation
      Kurashiki, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] シロイヌナズナ葉肉細胞における核光定位運動への葉緑体の関与

    • Author(s)
      田中怜、岩渕功誠、西村いくこ、高木慎吾
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 植物ビリンは葉緑体アンカーに関与するか

    • Author(s)
      高木慎吾
    • Organizer
      第15回植物オルガネラワークショップ
    • Place of Presentation
      オルガホール(岡山県)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] アクチン結合ドメインを持つキネシン様タンパク質遺伝子欠損シロイヌナズナの表現型解析

    • Author(s)
      光武翔、安原裕紀、高木慎吾
    • Organizer
      2013年生体運動研究合同班会議
    • Place of Presentation
      広島大学(広島県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of LINCs involved in the maintenance of nuclear morphology in Arabidopsis thaliana

    • Author(s)
      Y. Sakamoto and S. Takagi
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Phototropin2-dependent co-localization of mitochondria with chloroplasts in Arabidopsis thaliana mesophyll cells

    • Author(s)
      M.S. Islam and S. Takagi
    • Organizer
      平成24年度近畿植物学会
    • Place of Presentation
      近畿大学(奈良県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナにおける細胞核形態を維持するLINCの解析

    • Author(s)
      坂本勇貴、高木慎吾
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • Related Report
      2012 Final Research Report
  • [Remarks] 植物細胞生物学研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/takagi/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/takagi/index.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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