• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A flagella-derived structure in Chlorella?

Research Project

Project/Area Number 23657039
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionHiroshima University

Principal Investigator

YAMADA Takashi  広島大学, 大学院・先端物質科学研究科, 教授 (40230461)

Co-Investigator(Kenkyū-buntansha) FUJIE Makoto  広島大学, 大学院・先端物質科学研究科, 准教授 (20274110)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsオルガネラ / 細胞壁 / クロレラ / 鞭毛 / ゲノム情報 / 有性生殖 / 減数分裂 / 植物生理学
Research Abstract

Based on DNA microarray analysis, 103 flagella-related genes, 4 meiosis-related genes, and 22 housekeeping genes of Chlorella variabilis NC64A were characterized to be expressed under stress conditions. Two flagella-related genes (Ig174, eG176) were highly expressed under N-starvation and cold-stress conditions. Detailed expression patterns of these genes were further characterized by using qRT-PCR. With these genes as reference, expression of many other genes has been investigated.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (16 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (7 results) Remarks (1 results)

  • [Journal Article] Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses.2013

    • Author(s)
      Rakkhumkaew, N., Shibatani, S., Kawasaki, T.,Fujie, M., Yamada, T
    • Journal Title

      J. Biosci. Bioeng

      Volume: 115 Pages: 527-531

    • NAID

      110009611457

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hyaluronan synthesis in cultured tobacco cells (BY-2) expressing a chlorovirus enzyme: cytological studies.2013

    • Author(s)
      Rakkhumkaew, N., Shibatani, S., Kawasaki, T.,Fujie, M., Yamada, T
    • Journal Title

      Biotech. Bioeng

      Volume: 110 Pages: 1174-1179

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hyaluronan synthesis in cultured tobacco cells (BY-2) expressing a chlorovirus enzyme2013

    • Author(s)
      Rakkhumkaew, N., S. Shibatani, T. Kawasaki, M. Fujie, and T. Yamada
    • Journal Title

      Cytological studies. Biotech. Bioeng

      Volume: 110(4) Issue: 4 Pages: 1174-1179

    • DOI

      10.1002/bit.24783

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The genome of the polar eukaryotic microalga Coccomyxa subellipsoidea reveals traits of cold adaptation.2012

    • Author(s)
      Blanc G., Yamada, T. et al.
    • Journal Title

      Genome Biol

      Volume: 13 Issue: 5

    • DOI

      10.1186/gb-2012-13-5-r39

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses2012

    • Author(s)
      Rakkhumkaew, N., T. Kawasaki, M. Fujie, and T. Yamada
    • Journal Title

      J. Biosci. Bioeng

      Volume: 115(5) Issue: 5 Pages: 527-531

    • DOI

      10.1016/j.jbiosc.2012.11.012

    • NAID

      110009611457

    • Related Report
      2012 Annual Research Report
  • [Journal Article] The GC-rich mitochondrial and plastid genomes of the green alga Coccomyxa give insight into theevolution of organelle DNA nucleotide landscape2011

    • Author(s)
      Smith,D.R.,Burki,F.,Yamada,T.,Grimwood,J.,Grigoriev,I.V.,Van Etten,J.L.,Keeling, P.J.
    • Journal Title

      PLoS One

      Volume: 6(8) Issue: 8 Pages: e23624-e23624

    • DOI

      10.1371/journal.pone.0023624

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Giant viruses in the environment: their origins and evolution2011

    • Author(s)
      Yamada, T
    • Journal Title

      Curr. Opin.

      Volume: Virol.1 Pages: 58-62

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Giant viruses in the environment: their origins and evolution2011

    • Author(s)
      T. Yamada
    • Journal Title

      Curr Opin Virol

      Volume: 1 Issue: 1 Pages: 58-62

    • DOI

      10.1016/j.coviro.2011.05.008

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] クロレラ鞭毛関連遺伝子の発現解析2012

    • Author(s)
      熊谷徳泰,川崎 健、藤江 誠, 山田 隆
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-10-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Prolonged synthesis of hyaluronan by Chlorella cells infected with Chloroviruses2012

    • Author(s)
      Rakkhumkaew,N., Kawasaki, T., Fujie, M., Yamada, T
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-10-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Prolonged synthesis of hyaluronan by Chlorella cells infected with Chloroviruses.2012

    • Author(s)
      Numfon Rakkhumkaew他3名
    • Organizer
      第64回日本生物工学会大会
    • Related Report
      2012 Annual Research Report
  • [Presentation] クロレラ鞭毛関連遺伝子の発現解析2012

    • Author(s)
      熊谷徳泰,他3名
    • Organizer
      第64回日本生物工学会大会
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cytological study of hyaluronan synthesis in tobacco cultured-cells (BY-2) exerted by chlorovirus enzymes2011

    • Author(s)
      Rakkhumkaew, N., Shibatani, S., Kawasaki, T., Fujie, M., Yamada, T.
    • Organizer
      The 4thThailand-Japan International Academic Conference
    • Place of Presentation
      Tokyo, Japan.
    • Year and Date
      2011-11-06
    • Related Report
      2012 Final Research Report
  • [Presentation] Cytological study of hyaluronan synthesis in tobacco cultured -cells (BY-2) exerted by chlorovirus enzymes2011

    • Author(s)
      N. Rakkhumkaew, S. Shibatani, T. Kawasaki, M. Fujie, T. Yamada
    • Organizer
      The 4th Thailand-Japan International Academic Conference 2011
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular mechanism of hyaluronan synthesis on tobacco cutured-cells (BY-2) exerted vy chlorovirus enzymes2011

    • Author(s)
      N. Rakkhumkaew, S. Shibatani, T. Kawasaki, M. Fujie, T. Yamada
    • Organizer
      63th, Annual meeting of Japanese Society of Biotechnology
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/index.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-12-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi