A flagella-derived structure in Chlorella?

• Project/Area Number: 23657039
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Hiroshima University
• Principal Investigator: YAMADA Takashi 広島大学, 大学院・先端物質科学研究科, 教授 (40230461)
• Co-Investigator(Kenkyū-buntansha): FUJIE Makoto 広島大学, 大学院・先端物質科学研究科, 准教授 (20274110)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: オルガネラ / 細胞壁 / クロレラ / 鞭毛 / ゲノム情報 / 有性生殖 / 減数分裂 / 植物生理学

Research Abstract

Based on DNA microarray analysis, 103 flagella-related genes, 4 meiosis-related genes, and 22 housekeeping genes of Chlorella variabilis NC64A were characterized to be expressed under stress conditions. Two flagella-related genes (Ig174, eG176) were highly expressed under N-starvation and cold-stress conditions. Detailed expression patterns of these genes were further characterized by using qRT-PCR. With these genes as reference, expression of many other genes has been investigated.

Research Products

• [Journal Article] Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses. (2013)
  • Author(s): Rakkhumkaew, N., Shibatani, S., Kawasaki, T.,Fujie, M., Yamada, T
  • Journal Title: J. Biosci. Bioeng Volume: 115 Pages: 527-531
  • NAID: 110009611457
  • Peer Reviewed
• [Journal Article] Hyaluronan synthesis in cultured tobacco cells (BY-2) expressing a chlorovirus enzyme: cytological studies. (2013)
  • Author(s): Rakkhumkaew, N., Shibatani, S., Kawasaki, T.,Fujie, M., Yamada, T
  • Journal Title: Biotech. Bioeng Volume: 110 Pages: 1174-1179
  • Peer Reviewed
• [Journal Article] Hyaluronan synthesis in cultured tobacco cells (BY-2) expressing a chlorovirus enzyme (2013)
  • Author(s): Rakkhumkaew, N., S. Shibatani, T. Kawasaki, M. Fujie, and T. Yamada
  • Journal Title: Cytological studies. Biotech. Bioeng Volume: 110(4) Issue: 4 Pages: 1174-1179
  • DOI: 10.1002/bit.24783
  • Peer Reviewed
• [Journal Article] The genome of the polar eukaryotic microalga Coccomyxa subellipsoidea reveals traits of cold adaptation. (2012)
  • Author(s): Blanc G., Yamada, T. et al.
  • Journal Title: Genome Biol Volume: 13 Issue: 5 Pages: R39-R39
  • DOI: 10.1186/gb-2012-13-5-r39
  • Peer Reviewed
• [Journal Article] Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses (2012)
  • Author(s): Rakkhumkaew, N., T. Kawasaki, M. Fujie, and T. Yamada
  • Journal Title: J. Biosci. Bioeng Volume: 115(5) Issue: 5 Pages: 527-531
  • DOI: 10.1016/j.jbiosc.2012.11.012
  • NAID: 110009611457
• [Journal Article] The GC-rich mitochondrial and plastid genomes of the green alga Coccomyxa give insight into theevolution of organelle DNA nucleotide landscape (2011)
  • Author(s): Smith,D.R.,Burki,F.,Yamada,T.,Grimwood,J.,Grigoriev,I.V.,Van Etten,J.L.,Keeling, P.J.
  • Journal Title: PLoS One Volume: 6(8) Issue: 8 Pages: e23624-e23624
  • DOI: 10.1371/journal.pone.0023624
  • Peer Reviewed
• [Journal Article] Giant viruses in the environment: their origins and evolution (2011)
  • Author(s): Yamada, T
  • Journal Title: Curr. Opin. Volume: Virol.1 Pages: 58-62
  • Peer Reviewed
• [Journal Article] Giant viruses in the environment: their origins and evolution (2011)
  • Author(s): T. Yamada
  • Journal Title: Curr Opin Virol Volume: 1 Issue: 1 Pages: 58-62
  • DOI: 10.1016/j.coviro.2011.05.008
  • Peer Reviewed
• [Presentation] クロレラ鞭毛関連遺伝子の発現解析 (2012)
  • Author(s): 熊谷徳泰,川崎 健、藤江 誠, 山田 隆
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-10-24
• [Presentation] Prolonged synthesis of hyaluronan by Chlorella cells infected with Chloroviruses (2012)
  • Author(s): Rakkhumkaew,N., Kawasaki, T., Fujie, M., Yamada, T
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-10-24
• [Presentation] Prolonged synthesis of hyaluronan by Chlorella cells infected with Chloroviruses. (2012)
  • Author(s): Numfon Rakkhumkaew他３名
  • Organizer: 第６４回日本生物工学会大会
• [Presentation] クロレラ鞭毛関連遺伝子の発現解析 (2012)
  • Author(s): 熊谷徳泰，他３名
  • Organizer: 第６４回日本生物工学会大会
• [Presentation] Cytological study of hyaluronan synthesis in tobacco cultured-cells (BY-2) exerted by chlorovirus enzymes (2011)
  • Author(s): Rakkhumkaew, N., Shibatani, S., Kawasaki, T., Fujie, M., Yamada, T.
  • Organizer: The 4thThailand-Japan International Academic Conference
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 2011-11-06
• [Presentation] Cytological study of hyaluronan synthesis in tobacco cultured -cells (BY-2) exerted by chlorovirus enzymes (2011)
  • Author(s): N. Rakkhumkaew, S. Shibatani, T. Kawasaki, M. Fujie, T. Yamada
  • Organizer: The 4th Thailand-Japan International Academic Conference 2011
  • Place of Presentation: Tokyo, Japan
• [Presentation] Molecular mechanism of hyaluronan synthesis on tobacco cutured-cells (BY-2) exerted vy chlorovirus enzymes (2011)
  • Author(s): N. Rakkhumkaew, S. Shibatani, T. Kawasaki, M. Fujie, T. Yamada
  • Organizer: 63th, Annual meeting of Japanese Society of Biotechnology
  • Place of Presentation: Tokyo, Japan
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/mbiotech/ichikou/index.html