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Creation of artificial luminescent plant induced by luciferin biosynthesis system

Research Project

Project/Area Number 23657045
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

OHMIYA Yoshihiro  独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究部門長 (20223951)

Co-Investigator(Kenkyū-buntansha) MITSUDA Nobutaka  産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (80450667)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords組み換え植物 / ルシフェリン / ルシフェラーゼ / イメージング / 有用物質生産 / 生物発光 / ルシフェリン合成 / 遺伝子導入植物 / 蛍ルシフェラーゼ / ウミホタルルシフェラーゼ / 発光イメージング
Research Abstract

To establish the bioluminescent plants, we transfect the luciferase genes and the luciferin synthesis related genes to plants. At the first step, we established the firefly luciferase gene expressed and Cypridina luciferase expressed plants and confirmed

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Products Report
  • Research Products

    (7 results)

All 2018 2016 2015 2014 2013 Other

All Journal Article (2 results) Presentation (1 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 1 results)

  • [Journal Article] In Vivo Imaging Demonstrates ATP Release from Murine Keratinocytes and Its Involvement in Cutaneous Inflammation after Tape Stripping

    • Author(s)
      Takahashi T, Kimura Y, Niwa K, Ohmiya Y, Fujimura T, Yamasaki K, Aiba S
    • Journal Title

      J Invest Dermatol

      Volume: (in press)

    • Related Report
      2012 Final Research Report
  • [Journal Article] Interconversion of ketoprofen recognition in firefly luciferase-catalyzed enantioselective thioesterification reaction using from Pylocoeria miyako (PmL) and Hotaria parvura (HpL) just by mutating two amino acid residues

    • Author(s)
      Kato DI, Hiraishi Y, Maenaka M, Yokoyama K, Niwa K, Ohmiya Y, Takeo M, Negoro S
    • Journal Title

      J Biotechnol

      Volume: (in press)

    • Related Report
      2012 Final Research Report
  • [Presentation] Bioluminescence multicolor imaging using secreted and non-secreted luciferase2013

    • Author(s)
      OHMIYA YOSHIHIRO
    • Organizer
      組織培養学会年次発表会
    • Place of Presentation
      産総研共用行動(茨城県)
    • Year and Date
      2013-05-30
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] (英語)METHOD FOR PRODUCING VARGULA HILGE NDORFII LUCIFERASE (和訳)ウミホタルルシフェラーゼ生産法2018

    • Inventor(s)
      近江谷 克裕, 光田 展隆, 三谷 恭雄, 大島 良美
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-511605
    • Acquisition Date
      2018
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] METHOD FOR PRODUCING VARGULA HILGENDORFII LUCIFERASE2016

    • Inventor(s)
      近江谷 克裕, 光田 展隆, 三谷 恭雄, 大島 良美
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-511605
    • Filing Date
      2016-08-09
    • Description
      日本移行
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] ウミホタルルシフェラーゼ生産法2015

    • Inventor(s)
      近江谷 克裕, 光田 展隆, 三谷 恭雄, 大島 良美
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-03-26
    • Related Report
      Products Report
    • Overseas
  • [Patent(Industrial Property Rights)] ウミホタルルシフェラーゼ生産法2014

    • Inventor(s)
      近江谷 克裕, 光田 展隆, 三谷 恭雄, 大島 良美
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-076936
    • Filing Date
      2014-04-03
    • Related Report
      Products Report

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Published: 2011-08-05   Modified: 2019-07-29  

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