New assay to develop whole structure of embryo using presumptive epidermal region in Xenopus

• Project/Area Number: 23657047
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Shizuoka University
• Principal Investigator: KURODA Hiroki 静岡大学, 教育学部, 准教授 (70402229)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 疑似生命体 / 胚様構造 / ツメガエル / BCNEセンター / Nieuwkoopセンター / 初期発生 / アフリカツメガエル

Research Abstract

Using animal cap cells, which have pluripotency like ES and iPS cells, we tried to create overall structure of vertebrate embryo. First, we developed new method to create region having Spemann organizer activity by combination of the BCNE and Nieuwkoop centers. Then, we showed that this induced organizer enabled animal cap explants to generate anteroposterior axis formation.

Research Products

• [Journal Article] beta-adrenergic signaling promotes posteriorization in Xenopus early development Develop (2013)
  • Author(s): Shoko Mori, Yuki Moriyama, Kumiko Yoshikawa, Tomoyo Furukawa and Hiroki Kuroda
  • Journal Title: Growth Differ Volume: 55 Pages: 350-358
  • Peer Reviewed
• [Journal Article] beta-adrenergic signaling promotes posteriorization in Xenopus early development (2013)
  • Author(s): Shoko Mori
  • Journal Title: Development Growth and Differentiation Volume: 55 Issue: 3 Pages: 350-358
  • DOI: 10.1111/dgd.12046
  • Peer Reviewed
• [Journal Article] a method for generating dominant-negative inhibitors of the secretion of TGF-beta superfamily proteins (2012)
  • Author(s): Shinya Matsukawa, Yuki Moriyama, Tadayoshi Hayata, Haruka Sasaki, Yuzuru Ito, Makoto Asashima, and Hiroki KurodaKDEL tagging
  • Journal Title: Int. J. Dev. Biol Volume: 56 Pages: 351-356
  • Peer Reviewed
• [Presentation] KDEL tagging: a method for generating dominant-negative inhibitors of the secretion of TGF-beta (2012)
  • Author(s): 黒田裕樹
  • Organizer: superfamily proteins Xenopus Conference
  • Place of Presentation: ジアン半島、フランス
  • Year and Date: 2012-09-11
• [Presentation] KDEL tagging: a method for generating dominant-negative inhibitors of the secretion of TGF-beta superfamily proteins
  • Author(s): 黒田裕樹
  • Organizer: Xenopus Conference
  • Place of Presentation: Gians Peninsula, France