Occupying the space :A new phenomenon in cell attachment,and the elucidation of molecular mechanism

• Project/Area Number: 23657049
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Sapporo Medical University
• Principal Investigator: KOHNO Takayuki 札幌医科大学, 医学部, 講師 (10374563)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞接着 / 場所取り / 場所とり

Research Abstract

When suspended adherent monolayer mammalian cells are plated onto a culture dish,the cells disperse almost uniformly as single cells and attach to the surface of the plastic cell cultureware. Subsequently,the cells grow in the shape of a pavement,and finally the cell growth and motility arrest by“contactinhibition”.This phenomenon is known for more than half acentury.In this study,I found that a new phenomenon called“occupying the space” existed for the first time in this process to disperse the cells uniformly.Furthermore,I considered the generality of this finding.