• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Holographic Four-Dimensional Microscopy for Biomedical Diagnostics

Research Project

Project/Area Number 23657050
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionUniversity of Hyogo

Principal Investigator

SATO Kunihiro  兵庫県立大学, 工学(系)研究科(研究院), 教授 (40167432)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords顕微鏡技術 / デジタルホログラフィ / 3次元断層顕微鏡 / レンズレス / 超高分解能 / 生体計測 / 生体細胞 / 3次元断層顕微鏡 / 3次元顕微鏡 / 無歪高分解能 / 微生物 / 水中観察 / 大深度
Research Abstract

A lens-less holographic microscope has been developed for 4-D measurement of microorganisms swimming in water. Intensity and phase images with a high resolving power and with a large depth are precisely reconstructed for moving objects in air or even in dielectric media. High-resolution microscopic tomography has also been developed for optical measurement of living tissue by using the lens-less reflection-type holographic microscope. Experimental results demonstrate tomographic 3-D imaging with diffraction limited resolution. A new method has been proposed in order to develop a super-resolution lens-less holographic microscopy. Four or more holograms with different incident angles of illuminating light are recorded to generate one hologram with a large value of the numerical aperture (NA). NA of the generated hologram can be enlarged up to a value larger than 1, and a resolution of the microscopy can be improved up to a value higher than one half of the wave length.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (43 results)

All 2014 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 1 results) Presentation (26 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (9 results) (of which Overseas: 5 results)

  • [Journal Article] Instantaneous shape measurement of moving surface with high accuracy by one-shot digital holography2012

    • Author(s)
      K. Sato, Y. Iwayama
    • Journal Title

      Proc. of SPIE

      Volume: 8281

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Real-time recording and reconstruction of moving 3D images using electronic holography2012

    • Author(s)
      K. Furuichi, K. Sato
    • Journal Title

      Proc. of SPIE

      Volume: 8281

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Lensless holographic microscopy with high-resolving power for 4D measurement of micro-organism swimming in water2012

    • Author(s)
      M. Otani, K. Sato
    • Journal Title

      Proc. of SPIE

      Volume: 8227 Pages: 2901-2906

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Numerical Reconstruction of Hi-Resolution Microscopic Images for Lens-Less Holographic Microscopy2012

    • Author(s)
      K. Sato
    • Journal Title

      International Journal of Intelligent Computing in Medical Sciences and Image Processing

      Volume: 4 Pages: 77-86

    • Related Report
      2013 Final Research Report
  • [Journal Article] Numerical reconstruction of high-resolution microscopic images for lens-less holographic microscopy2012

    • Author(s)
      Kunihiro Sato
    • Journal Title

      International Journal of Intelligent Computing in Medical Sciences and Image Processing

      Volume: Vol. 4 Pages: 77-86

    • Related Report
      2012 Research-status Report
  • [Journal Article] Numerical Reconstruction of Hi-Resolution Microscopic Images for Lens-Less Holographic Microscopy2012

    • Author(s)
      K. Sato
    • Journal Title

      International Journal of IC-MED

      Volume: Vol.4 Pages: 77-86

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lens-less holographic microscope with highresolving powerandno-distortion2011

    • Author(s)
      K. Sato and O. Murata
    • Journal Title

      Proc. of SPIE

      Volume: 7904 Pages: 11-18

    • Related Report
      2013 Final Research Report
  • [Journal Article] Instantaneous measurement of surface shape with high accuracy by one-shot digital holography2011

    • Author(s)
      Y. Iwayama and K. Sato
    • Journal Title

      Proc. of SPIE

      Volume: 7904 Pages: 81-88

    • Related Report
      2013 Final Research Report
  • [Presentation] 生体光計測のためのレンズレスホログラフィック顕微鏡2014

    • Author(s)
      佐藤邦弘
    • Organizer
      電気四学会関西支部専門講習会
    • Place of Presentation
      大阪中央電気ぐらく部
    • Year and Date
      2014-01-24
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Invited
  • [Presentation] ホログラフィによる生体計測用高分解能3次元断層顕微鏡を用いた生体細胞の観察2013

    • Author(s)
      横山潤平, 大谷真由, 佐藤邦弘
    • Organizer
      日本光学会年次講演会
    • Place of Presentation
      奈良公会堂
    • Year and Date
      2013-11-14
    • Related Report
      2013 Final Research Report
  • [Presentation] レンズレスホログラフィック顕微鏡の多方向照明による超高分解能化2013

    • Author(s)
      横山潤平, 大谷真由, 佐藤邦弘
    • Organizer
      日本光学会年次講演会
    • Place of Presentation
      奈良公会堂
    • Year and Date
      2013-11-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Real-time recording and real-time display of moving 3-D images by electronic holography2013

    • Author(s)
      K. Kawachi, K. Furuichi, K. Sato
    • Organizer
      The 3ed International Symposium on Himeji Initiative in Computational Medical and Health Technology
    • Year and Date
      2013-07-26
    • Related Report
      2013 Final Research Report
  • [Presentation] High-resolution Lens-less Holographic Microscopy for 4-D Measurement of Transparent Microorganism Swimming in Water2013

    • Author(s)
      J. Yokoyama, M. Otani, K. Sato
    • Organizer
      The 3ed International Symposium on Himeji Initiative in Computational Medical and Health Technology
    • Year and Date
      2013-07-26
    • Related Report
      2013 Final Research Report
  • [Presentation] ワンショットディジタルホログラフィによる高速運動物体のための高精度形状計測2012

    • Author(s)
      佐藤邦弘, 岩山義秀
    • Organizer
      日本光学会
    • Place of Presentation
      タワーホール船越
    • Year and Date
      2012-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 生体計測用の細胞レベル高分解能3次元断層顕微鏡の開発2012

    • Author(s)
      古市和稔, 佐藤邦弘
    • Organizer
      日本光学会
    • Place of Presentation
      タワーホール船越
    • Year and Date
      2012-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Holographic microscope by one-shot digital holography2011

    • Author(s)
      M. Otani and K.Sato
    • Organizer
      OSA Optics & Photonics Congress(reviewed)
    • Related Report
      2013 Final Research Report
  • [Presentation] 室内照明下における運動物体3次元像の実時間撮像と実時間表示2011

    • Author(s)
      古市和稔, 佐藤邦弘
    • Organizer
      3次元画像コンファレンス2011講演論文集
    • Related Report
      2013 Final Research Report
  • [Presentation] Color 3-D Display and Color 3-D Video Camera by Electronic Holography2011

    • Author(s)
      K. Sato
    • Organizer
      The 11th International Meeting on Information Display(招待講演)
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2011 Research-status Report
  • [Presentation] Lens-less 4-D microscopy for measurement of moving microorganism by the one-shot digital holography2011

    • Author(s)
      K. Sato
    • Organizer
      International Workshop on Holography and Related Technologies 2011(招待講演)
    • Place of Presentation
      Utsunomiya Univ. (Tochigi), Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Holographic microscope by one-shot digital holography2011

    • Author(s)
      M. Otani, K. Sato
    • Organizer
      OSA Optics & Photonics Congress
    • Place of Presentation
      Univ. of Tokyo (Tokyo), Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] High-resolution Lens-less Holographic Microscopy for 4-D Measurement of Transparent Microorganism Swimming in Water

    • Author(s)
      J. Yokoyama, M. Otani, K. Sato
    • Organizer
      The 3rd International Symposium on Himeji Initiative in Computational Medical and Health Technology
    • Place of Presentation
      Himeji, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] Real-time recording and real-time display of moving 3-D images by electronic holography

    • Author(s)
      K. Kawachi, K. Furuichi, K. Sato
    • Organizer
      The 3rd International Symposium on Himeji Initiative in Computational Medical and Health Technology
    • Place of Presentation
      Himeji, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] レンズレスホログラフィック顕微鏡の多方向照明による超高分解能化

    • Author(s)
      横山潤平,大谷真由,佐藤邦弘
    • Organizer
      日本光学会年次学術講演会
    • Place of Presentation
      奈良県新公会堂
    • Related Report
      2013 Annual Research Report
  • [Presentation] ホログラフィによる生体計測用高分解能3次元断層顕微鏡を用いた生体細胞の観察

    • Author(s)
      河内賢太,古市和稔,佐藤邦弘
    • Organizer
      日本光学会年次学術講演会
    • Place of Presentation
      奈良県新公会堂
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生体計測用の細胞レベル高分解能3次元断層顕微鏡の開発

    • Author(s)
      古市和稔、佐藤邦弘
    • Organizer
      日本光学会
    • Place of Presentation
      タワーホール船越
    • Related Report
      2012 Research-status Report
  • [Presentation] ワンショットディジタルホログラフィによる高速運動物体のための高精度形状計測

    • Author(s)
      佐藤邦弘、岩山義秀
    • Organizer
      日本光学会
    • Place of Presentation
      タワーホール船越
    • Related Report
      2012 Research-status Report
  • [Presentation] 室内照明下における運動物体3次元像の実時間撮像と実時間表示

    • Author(s)
      古市和稔,佐藤邦弘
    • Organizer
      3次元画像コンファレンス2011
    • Place of Presentation
      京都工芸繊維大(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] 媒質中高分解能3次元観察のためのレンズレス・ホログラフィック顕微鏡

    • Author(s)
      大谷真由,佐藤邦弘
    • Organizer
      3次元画像コンファレンス2011
    • Place of Presentation
      京都工芸繊維大(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] ディジタル・ホログラフィを用いた運動物体の大深度精密形状計測

    • Author(s)
      岩山義秀、佐藤邦弘
    • Organizer
      電気関係学会関西支部連合大会
    • Place of Presentation
      兵庫県立大学(兵庫県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 電子ホログラフィによる3次元動画像の実時間撮像と表示

    • Author(s)
      古市和稔,佐藤邦弘
    • Organizer
      電気関係学会関西支部連合大会
    • Place of Presentation
      兵庫県立大学(兵庫県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 水中微生物の4次元観察を可能にするレンズレス・ホログラフィック顕微鏡

    • Author(s)
      大谷真由,佐藤邦弘
    • Organizer
      電気関係学会関西支部連合大会
    • Place of Presentation
      兵庫県立大学(兵庫県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 大深度運動物体のためのワンショットディジタルホログラフィによる精密表面形状計測

    • Author(s)
      岩山義秀、佐藤邦弘
    • Organizer
      日本光学会年次学術講演会
    • Place of Presentation
      大阪大学(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] 電子ホログラフィによる室内照明下における3次元動画像の実時間撮像と実時間表示

    • Author(s)
      古市和稔,佐藤邦弘
    • Organizer
      日本光学会年次学術講演会
    • Place of Presentation
      大阪大学(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] 水中微生物の高分解能4次元計測のためのレンズレス・ホログラフィック顕微鏡

    • Author(s)
      大谷真由,佐藤邦弘
    • Organizer
      日本光学会年次学術講演会
    • Place of Presentation
      大阪大学(大阪府)
    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] ホログラフィック顕微鏡, 高分解能画像用のホログラフィックデータ取得方法, および高分解能ホログラフィック画像再生法2013

    • Inventor(s)
      佐藤邦弘
    • Industrial Property Rights Holder
      兵庫県
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-223761
    • Filing Date
      2013-10-28
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] ホログラフィック断層顕微鏡, ホログラフィック断層画像生成法, およびホログラフィック断層画像用のデータ取得方法2013

    • Inventor(s)
      佐藤邦弘
    • Industrial Property Rights Holder
      兵庫県
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-10-04
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] GENERATION METHOD FOR COMPLEX AMPLITUDE IN-LINE HOLOGRAM AND IMAGE RECORDING DEVICE USING SAID SAID METHOD2013

    • Inventor(s)
      Kunihiro Sato
    • Industrial Property Rights Holder
      Hyogo Prefectural Government
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2013-04-09
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] ホログラフィック顕微鏡、高分解能画像用のホログラムデータ取得方法、および高分解能ホログラム画像再生方法2013

    • Inventor(s)
      佐藤邦弘
    • Industrial Property Rights Holder
      兵庫県
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-223761
    • Filing Date
      2013-10-28
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] ホログラフィック断層顕微鏡、ホログラフィック断層画像生成方法、およびホログラフィック断層画像用のデータ取得方法2013

    • Inventor(s)
      佐藤邦弘
    • Industrial Property Rights Holder
      兵庫県
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-10-04
    • Related Report
      2013 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] ホログラフィック断層顕微鏡, ホログラフィック断層画像生成法, およびホログラフィック断層画像用のデータ取得方法2012

    • Inventor(s)
      佐藤邦弘
    • Industrial Property Rights Holder
      兵庫県
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-223690
    • Filing Date
      2012-10-05
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] ホログラフィック断層顕微鏡、ホログラフィック断層画像生成方法、およびホログラフィック断層画像用のデータ取得方法2012

    • Inventor(s)
      佐藤邦弘
    • Industrial Property Rights Holder
      兵庫県
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-223690
    • Filing Date
      2012-10-05
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 3次元形状計測および3次元形状計測装置2012

    • Inventor(s)
      佐藤邦弘
    • Industrial Property Rights Holder
      兵庫県
    • Filing Date
      2012
    • Related Report
      2011 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 微小被写体のホログラム画像記録方法、高分解能画像再生用ホログラム作成方法、画像再生方法、およびホログラフィック顕微鏡2011

    • Inventor(s)
      佐藤邦弘
    • Industrial Property Rights Holder
      兵庫県
    • Filing Date
      2011
    • Related Report
      2011 Research-status Report
    • Overseas

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi