| Project/Area Number |
23657051
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Morphology/Structure
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
TOYOOKA Kiminori 独立行政法人理化学研究所, 機能開発研究グループ, 上級研究員 (10360596)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
NISHIKAWA Mayuko 独立行政法人理化学研究所, 機能開発研究グループ, 技師 (80550376)
MOCHIDA Keiichi 独立行政法人理化学研究所, バイオマス研究基盤チーム, 上級研究員 (90387960)
|
|---|
| Research Collaborator |
永田 典子 日本女子大学, 理学部, 准教授
朽名 夏麿 東京大学, 大学院・新領域創成科学研究科, 助教
桧垣 匠 東京大学, 大学院・新領域創成科学研究科, 助教
吉田 拓広 独立行政法人理化学研究所, ゲノム情報統合ユニット, テクニカルスタッフ
櫻井 哲也 独立行政法人理化学研究所, ゲノム情報統合ユニット, ユニットリーダー
若崎 眞由美 独立行政法人理化学研究所, 機能開発研究グループ, テクニカルスタッフ
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 電子顕微鏡 / 高圧凍結技法 / 電子地図 / オルガネラ / 超微形態 |
|---|
| Research Abstract |
Transmission electron microscopy (TEM) is important for high-resolution ultrastructure observation for organelles, cell and tissues. However, it is unsuitable for comprehensive analyses. I constructed TEM atlas to achieve an exhaustive ultrastructural analysis of organelles in model plants by using high-pressure freezing (HFP) and TEM techniques. To take wide and high-resolution TEM pictures, Arabidopsis roots and BY2 cells were frozen by HFP machine and then fix. After embedded in resin, large ultrathin sections were put on a hole grid. Then we took TEM pictures by auto-digital TEM recoding system. I succeed in records more than 30 thousand images. We developed auto-photomerge program for TEM pictures and several thousands of pictures were automatically merged. Using these wide-high resolution pictures, I have developed a web-based 2D map that can be zoomed and searched.
|
