Revolution of Phylogenetic Analysis with Next Generation Sequencers

Research Project

Project/Area Number	23657063
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Biodiversity/Systematics
Research Institution	Kanazawa University
Principal Investigator	NISHIYAMA Tomoaki 金沢大学, 学際科学実験センター, 助教 (50390688)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	ゲノム / トランスクリプトーム / 系統解析 / 全ゲノム解析 / オルガネラ / RNA-seq
Research Abstract	This research aimed at sequencing both the genome and the transcriptome of organisms that occupy pivotal position in the phylogeny to allow resolution of difficult phylogenetic problems such as deep branches with high statistical confidence. A liverwort species, Jungermannia infusca, with an estimated genome size of 386 Mb was sequenced and assembled, indicating that sequencing nuclear genome of less than 400 Mb is realistically doable, and could be less expensive than focusing RNA-seq when one want to obtain low-expression level genes and very long transcripts.

Report

(4 results)