Revolution of Phylogenetic Analysis with Next Generation Sequencers

• Project/Area Number: 23657063
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Kanazawa University
• Principal Investigator: NISHIYAMA Tomoaki 金沢大学, 学際科学実験センター, 助教 (50390688)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ゲノム / トランスクリプトーム / 系統解析 / 全ゲノム解析 / オルガネラ / RNA-seq

Research Abstract

This research aimed at sequencing both the genome and the transcriptome of organisms that occupy pivotal position in the phylogeny to allow resolution of difficult phylogenetic problems such as deep branches with high statistical confidence. A liverwort species, Jungermannia infusca, with an estimated genome size of 386 Mb was sequenced and assembled, indicating that sequencing nuclear genome of less than 400 Mb is realistically doable, and could be less expensive than focusing RNA-seq when one want to obtain low-expression level genes and very long transcripts.

Research Products

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  • URL: http://koke.asrc.kanazawa-u.ac.jp/