Microbial diversity and rare-biosphere in terrestrial subsurface environments revealed by high-throughput sequencing technologies

• Project/Area Number: 23657069
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: TAMAKI Hideyuki 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (00421842)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 群集・生態系多様性 / 未知微生物系統群 / 陸域地下圏 / 地下圏 / 次世代シークエンス / 微生物 / 多様性 / レアバイオスフィア / 生物地球化学 / 16S rRNA / 微生物生態 / 未知系統群 / パイロシークエンス / 16S rRNA遺伝子 / 生物多様性

Research Abstract

This study revealed the hidden microbial diversity and rare-biosphere in unexplored terrestrial subsurface environments by using massively parallel 16S rRNA sequencing via high-throughput sequencing technology. Indeed, the state of the arts method provide

Research Products

• [Journal Article] 大量シークエンス情報解析時代の未知微生物探索 (2013)
  • Author(s): 玉木秀幸
  • Journal Title: 日本乳酸菌学会誌 Volume: 24 Pages: 26-33
  • NAID: 10031130745
• [Journal Article] Methanothermobacter tenebrarum sp. nov., a hydrogenotrophic thermophilic methanogen isolated from gas-associated formation water of a natural gas field in Japan (2013)
  • Author(s): Nakamura, K., A. Takahashi, C. Mori, H. Tamaki, H. Mochimaru, K. Nakamura, K. Takamizawa, and, Y. Kamagata
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology. Volume: 63 Issue: Pt_2 Pages: 715-722
  • DOI: 10.1099/ijs.0.041681-0
  • Peer Reviewed
• [Journal Article] Metagenomic and Biochemical Characterizations of Sulfur Oxidation Metabolism in Uncultured Large Sausage-Shaped Bacterium in Hot Spring Microbial Mats (2012)
  • Author(s): Tamazawa S, Takasaki K, Tamaki H, Kamagata Y, Hanada S
  • Journal Title: PLoS One Volume: 7 Issue: 11 Pages: e49793-e49793
  • DOI: 10.1371/journal.pone.0049793
  • Peer Reviewed
• [Journal Article] 環境ゲノム情報時代の未知微生物探索研究 (2012)
  • Author(s): 玉木秀幸、鎌形洋一
  • Journal Title: 化学と生物 Volume: 50 Pages: 730-741
  • NAID: 10031123226
  • Peer Reviewed
• [Journal Article] Analysis of 16S rRNA amplicon sequencing options on the Roche/454 Next-Generation Titanium Sequencing platform (2011)
  • Author(s): Tamaki H, Wright CL, Li X, Lin Q, Hwang C, Wang S, Thimmapuram J, Kamagata Y & Liu WT
  • Journal Title: PLoS One Volume: 6 Issue: 9 Pages: e25263-e25263
  • DOI: 10.1371/journal.pone.0025263
  • Peer Reviewed
• [Journal Article] A six well plate method: less laborious and effective method for cultivation of obligate anaerobic microorganisms (2011)
  • Author(s): Nakamura K, Tamaki H, Kang MS, Mochimaru H, Lee ST and Kamagata Y
  • Journal Title: Microbes Environ Volume: 26 Pages: 301-306
  • NAID: 10029978006
  • Peer Reviewed
• [Journal Article] Candidatus Methanogranum caenicola: a novel methanogen from the anaerobic digested sludge, and proposal of Methanomassiliicoccaceae fam. nov. and Methanomassiliicoccales ord. nov., for a methanogenic lineage of the class Thermoplasmata, Microbes and Envir
  • Author(s): Iino T, Tamaki H, Tamazawa S, Ueno Y, Ohkuma M, Suzuki K, Igarashi Y, Haruta S
  • Journal Title: 受理済
  • Peer Reviewed
• [Presentation] Correlating metabolically active microbial communities with geochemistry in an unexplored terrestrial subsurface environment, glacial deposit (2013)
  • Author(s): 山本京介、玉木秀幸ほか
  • Organizer: 98th Ecological Society of America Annual Meeting
  • Place of Presentation: ミネソタ(米国)
• [Presentation] Correlating metabolically active microbial communities with geochemistry in an unexplored terrestrial subsurface environment, glacial deposit (2013)
  • Author(s): 山本京祐、玉木秀幸ほか
  • Organizer: 98th Ecological Society of America Annual Meeting
  • Place of Presentation: Minneapolis, USA
• [Presentation] 関東平野の沖積層において新たに発見された陸域における微生物による嫌気的メタン酸化活動 (2012)
  • Author(s): 竹内美緒、吉岡秀佳、徐維那、田辺晋、玉木秀幸ほか
  • Organizer: 第22回環境地質学シンポジウム
  • Place of Presentation: 産業技術総合研究所(茨城県)
  • Year and Date: 2012-12-07
• [Presentation] 大量シークエンス情報解析時代の未知微生物探索 (2012)
  • Author(s): 玉木秀幸
  • Organizer: 2012 年度日本乳酸菌学会秋期セミナー
  • Place of Presentation: 明治大学(東京都)
  • Year and Date: 2012-11-30
• [Presentation] Close Encounters of the Third Kind in Microbial Ecology (2012)
  • Author(s): 玉木秀幸
  • Organizer: イリノイ大学環境土木工学科分子生態学セミナー
  • Place of Presentation: イリノイ大学(米国)
  • Year and Date: 2012-11-09
• [Presentation] Diversity and activity of methanogenic microbial community in a high-temperature biodegraded oil field (2012)
  • Author(s): 持丸華子、眞弓大介、吉岡秀佳、坂田将、玉木秀幸 、鎌形洋一
  • Organizer: 日本微生物生態学会第28回大会
  • Place of Presentation: 豊橋技術科学大学(愛知県)
  • Year and Date: 2012-09-20
• [Presentation] 第三 種 接近 遭 遇 -Close Encounters of the Third Kind in Microbial Ecology-、 (2012)
  • Author(s): 玉木秀幸
  • Organizer: 日本微生物生態学会第28回大会若手交流会
  • Place of Presentation: 豊橋技術科学大学(愛知県)
  • Year and Date: 2012-09-19
• [Presentation] Methanogenic diversity and activity in a high-temperature biodegraded oil field (2012)
  • Author(s): 持丸華子、眞弓大介、吉岡秀佳、坂田将、玉 木 秀 幸 、鎌 形 洋 一
  • Organizer: 14th International Society for Microbial Ecology
  • Place of Presentation: コペンハーゲン(デンマーク)
  • Year and Date: 2012-08-21
• [Presentation] 第三種接近遭遇 -Close Encounters of the Third Kind in Microbial Ecology-
  • Author(s): 玉木秀幸
  • Organizer: 日本微生物生態学会第28回大会若手交流会
  • Place of Presentation: 豊橋技術科学大学
  • Invited
• [Presentation] 大量シークエンス情報解析時代の未知微生物探索
  • Author(s): 玉木秀幸
  • Organizer: 2012年度日本乳酸菌学会秋期セミナー
  • Place of Presentation: 明治大学
  • Invited
• [Presentation] Close Encounters of the Third Kind in Microbial Ecology
  • Author(s): 玉木秀幸
  • Organizer: 米国イリノイ大学環境分子生態学セミナー
  • Place of Presentation: 米国イリノイ大学
  • Invited
• [Presentation] 関東平野の沖積層において新たに発見された陸域における微生物による嫌気的メタン酸化活動
  • Author(s): 竹内美緒、吉岡秀佳、徐維那、田邉晋、玉木秀幸ほか
  • Organizer: 第２２回環境地質学シンポジウム
  • Place of Presentation: つくば市
• [Presentation] Diversity and activity of methanogenic microbial community in a high-temperature biodegraded oil field
  • Author(s): 持丸華子、眞弓大介、吉岡秀佳、坂田将、玉木秀幸、鎌形洋一
  • Organizer: 日本微生物生態学会第28回大会
  • Place of Presentation: 豊橋技術科学大学
• [Presentation] Methanogenic diversity and activity in a high-temperature biodegraded oil field
  • Author(s): 持丸華子、眞弓大介、吉岡秀佳、坂田将、玉木秀幸、鎌形洋一
  • Organizer: 14th International Society for Microbial Ecology
  • Place of Presentation: Copenhagen, Denmark