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A functional study on the tRNA mimicry complexes in genetic decoding.

Research Project

Project/Area Number 23657071
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

ITO Koichi  東京大学, 新領域創成科学研究科, 教授 (10262073)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsタンパク質合成 / tRNA分子擬態 / 遺伝暗号 / mRNA品質管理 / Gタンパク質 / リボソーム / 翻訳終結 / 翻訳調節 / タンパクs質合成
Research Abstract

In order to elucidate the molecular characteristics of tRNA-mimicking proteins involved in genetic decoding and mRNA surveillance, we have conducted following experiments. (i) Structural and Biochemical analyses for the complexes of omnipotent archaeal translation elongation factor (aEF1alpha) with tRNA mimicry proteins (aRF1, aPelota). (ii) Genetic analyses for the functionality of tRNA-mimicking complex on the ribosome. These analyses revealed that the specific regions are located in the binding interface of the tRNA-mimicking complexes and that a novel functional site adjacent to the codon recognition site, on the tip domain that mimics the tRNA anticodon-loop is crucial for their functionality. These novel domains likely participate in decoding and mRNA surveillance, coupled with the GTPase switch function on the ribosome.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (28 results)

All 2014 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (16 results) Book (4 results) Remarks (2 results)

  • [Journal Article] A Genetic Approach for Analyzing the Co-operative Function of the tRNA Mimicry Complex, eRF1/eRF3, in Translation Termination on the Ribosome2014

    • Author(s)
      Wada M, Ito K
    • Journal Title

      Nucleic Acids Res

      Volume: (in press) Issue: 12 Pages: 7851-7866

    • DOI

      10.1093/nar/gku493

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Single Amino Acid Substitution Alters Omnipotent eRF1 of Dileptus to Euplotes-type Dualpotent eRF12013

    • Author(s)
      Li Y, Kim OT, Ito K, Saito K, Suzaki T,Harumoto T.
    • Journal Title

      Standard Codon Usage May be Advantageous in Raptorial Ciliates. Protist.

      Volume: 164 Issue: 3 Pages: 440-449

    • DOI

      10.1016/j.protis.2013.02.004

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cell growth control by stable Rbg2/Gir2 complex formation under amino acid starvation.2013

    • Author(s)
      Ishikawa, K
    • Journal Title

      Genes Cells

      Volume: 18 Issue: 10 Pages: 859-872

    • DOI

      10.1111/gtc.12082

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for translation termination by archaeal RF1 and GTP-bound EF1α complex.2012

    • Author(s)
      Kan Kobayashi, Kazuki Saito, Ryuichiro Ishitani, Koichi Ito, Osamu Nureki
    • Journal Title

      Nucleic Acids Res.

      Volume: 40 Issue: 18 Pages: 9319-9328

    • DOI

      10.1093/nar/gks660

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] tRNA mimicry in translation termination and beyond.2011

    • Author(s)
      Nakamura, Y., Ito, K.
    • Journal Title

      Wiley Interdiscip Rev (WIREs) RNA.

      Volume: 2 Issue: 5 Pages: 647-668

    • DOI

      10.1002/wrna.81

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Single molecule imaging of the trans-translation entry process via anch oring of the tagged ribosome.2011

    • Author(s)
      Zhou, Z-P., Shimizu, Y., Tadakuma, H.*, Taguchi, H., Ito, K., Ueda, T.
    • Journal Title

      J. Biochem.

      Volume: 149 Issue: 5 Pages: 609-618

    • DOI

      10.1093/jb/mvr010

    • NAID

      10030585460

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 終止コドン解読におけるeRF1とeRF3 の共役の解析2013

    • Author(s)
      斎藤和紀
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド 兵庫県
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 出芽酵母におけるEF-1αホモログG タンパク質Ski7 の機能ドメイン解析2013

    • Author(s)
      堀川航
    • Organizer
      第15回日本RNA 学会年会
    • Place of Presentation
      愛媛県民文化会館・ひめぎんホール 松山市道後
    • Year and Date
      2013-07-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 終止コドン解読におけるeRF1とeRF3の共役の解析2013

    • Author(s)
      斎藤和紀、伊藤耕一
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド・兵庫県
    • Related Report
      2013 Annual Research Report
  • [Presentation] 出芽酵母におけるEF-1αホモログGタンパク質Ski7の機能ドメイン解析2013

    • Author(s)
      堀川航、和田美紀、伊藤耕一
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      愛媛県民文化会館・ひめぎんホール 松山市道後
    • Related Report
      2013 Annual Research Report
  • [Presentation] 古細菌由来 aRF1-aEF1α-GTP 複合体による翻訳終結機構の 構造基盤2013

    • Author(s)
      小林 幹, 斎藤 和紀, 石谷 隆一郎, 伊藤 耕一, 濡木 理
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      愛媛県民文化会館・ひめぎんホール 松山市道後
    • Related Report
      2013 Annual Research Report
  • [Presentation] Analysis of stop codon decoding in eukaryotes2012

    • Author(s)
      Saito K
    • Organizer
      第35回 日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 3' to 5' mRNA degradation activating factor SKI7 contains a conserved functional motif in its N-terminal domain2012

    • Author(s)
      Horikawa W
    • Organizer
      第35回 日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of stop codon decoding in eukaryotes.2012

    • Author(s)
      Saito K., Ito K.
    • Organizer
      第35回 日本分子生物学会年会
    • Place of Presentation
      福岡、福岡国際会議場、マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Structural basis for translation termination by archaeal RF1 and GTP-bound EF1α complex2012

    • Author(s)
      Kan Kobayashi, Kazuki Saito, Ryuichiro Ishitani, Koichi Ito, and Osamu Nureki
    • Organizer
      XXIV tRNA Conference
    • Place of Presentation
      Olmué, Chile.
    • Related Report
      2012 Research-status Report
  • [Presentation] Structural basis for mRNA surveillance by Archaeal Pelota and GTP-bound EF1alpha complex2012

    • Author(s)
      Ryuichiro Ishitani, Kan Kobayashi, Izumi Kikuno, Kazushige Kuroha, Kazuki Saito, Koichi Ito, Toshifumi Inada, and Osamu Nureki
    • Organizer
      XXIV tRNA Conference
    • Place of Presentation
      Olmué, Chile.
    • Related Report
      2012 Research-status Report
  • [Presentation] 3' to 5' mRNA degradation activating factor SKI7 contains a conserved functional motif in its N-terminal domain.2012

    • Author(s)
      Horikawa W., Wada M., Ito K.
    • Organizer
      第35回 日本分子生物学会年会
    • Place of Presentation
      福岡、福岡国際会議場、マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Omnipotent Role of Archaeal Elongation Factor 1 Alpha in Translation Elongation, and Termination, and Quality Control of Protein Synthesis2011

    • Author(s)
      Saito K
    • Organizer
      The 16th Annual Meeting of The RNA Society
    • Place of Presentation
      Kyoto, Kyoto International Conference Center
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] Genetic Analyses of Critical Sites for eRF1-eRF3 Interplay in Translation Termination2011

    • Author(s)
      Wada M
    • Place of Presentation
      Kyoto, Kyoto International Conference Center
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] Structural Basis for Translation Termination by Archaeal RF1 and GTP-bound EF1α Complex2011

    • Author(s)
      Kobayashi K
    • Organizer
      The 16th Annual Meeting of The RNA Society
    • Place of Presentation
      Kyoto, Kyoto International Conference Center
    • Related Report
      2011 Research-status Report
  • [Presentation] Archaeal elongation factor acts as an omnipotent carrier GTPase in translational elongation, termination and mRNA surveillance2011

    • Author(s)
      Nureki O
    • Organizer
      The 16th Annual Meeting of The RNA Society
    • Place of Presentation
      Kyoto, Kyoto International Conference Center
    • Related Report
      2011 Research-status Report
  • [Presentation] A Translational Fine-tuning by The Drg2-GTPase/Dfrp2 Complex2011

    • Author(s)
      Ishikawa K
    • Organizer
      The 16th Annual Meeting of The RNA Society
    • Place of Presentation
      Kyoto, Kyoto International Conference Center
    • Related Report
      2011 Research-status Report
  • [Book] Translation Elongation, In Werner Dubitzky, Olaf Wolkenhauer, Encyclopedia of systems biology2013

    • Author(s)
      Asano, K. Ito, K(Kwang-Hyun Cho, Hiroki Yokota (Eds.))
    • Publisher
      New York : Springer
    • Related Report
      2013 Final Research Report
  • [Book] Translation Termination, In Werner Dubitzky, Olaf Wolkenhauer, Kwang-Hyun Cho, Hiroki Yokota (Eds.), Encyclopedia of systems biology2013

    • Author(s)
      Saito, K. Ito, K
    • Publisher
      New York : Springer
    • Related Report
      2013 Final Research Report
  • [Book] Ribosome Recycling, In Werner Dubitzky, Olaf Wolkenhauer, Encyclopedia of systems biology2013

    • Author(s)
      Saito, K. Ito, K(Kwang-Hyun Cho, Hiroki Yokota (Eds.))
    • Publisher
      New York : Springer
    • Related Report
      2013 Final Research Report
  • [Book] Evolution of Elongation Factor 1 Alpha, In Werner Dubitzky, Olaf Wolkenhauer, Encyclopedia of systems biology2013

    • Author(s)
      Saito, K. Ito, K(Kwang-Hyun Cho, Hiroki Yokota (Eds.))
    • Publisher
      New York : Springer
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.k.u-tokyo.ac.jp/mgs/mgs_info/molgenet/Home.html

    • Related Report
      2013 Final Research Report
  • [Remarks] なし。

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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