Specific host recognition mechanisms of marine virus, a dinoflagellate-infecting virus

• Project/Area Number: 23657076
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: University of Miyazaki (2012); Osaka University (2011)
• Principal Investigator: WADA Kei 宮崎大学, テニュアトラック推進機構, 助教 (80379304)
• Co-Investigator(Kenkyū-buntansha): FUKUYAMA Keiichi 大阪大学, 大学院・理学研究科, 教授 (80032283)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 分子認識および相互作用 / ウイルス / 赤潮 / 特異的認識 / 構造 / X線結晶構造解析

Research Abstract

Heterosigma akashiwo virus (HaV) is a large virus specifically infecting the harmful bloom-forming flagellate Heterosigma akashiwo. Based on field surveys and cross-reactivity tests between the host clones and HaV clones, suggesting that this strain-dependent infection may play an important role in determining the clonal composition and maintaining the intraspecies diversity of natural H. akashiwo populations. In this study, to elucidate the mechanism of the specific host infection, the Vp183 protein, the specific host recognition protein, was overproduced.

Research Products

• [Journal Article] Wada, K., Kimura, K., Hasegawa, H.,Fukuyama., K and Nagasaki, K (2013)
  • Author(s): Wada, K., Kimura, K., Hasegawa, H.,Fukuyama., K and Nagasaki, K
  • Journal Title: Microbes Environ Volume: 27 Pages: 483-489
  • Peer Reviewed
• [Journal Article] hermal denaturation andrenaturation of γ-glutamyltranspeptidase of Escherichiacoli (2013)
  • Author(s): Ho T.V, Kamei, K. Wada, K., Fukuyama,K. and Suzuki, H
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 77 Pages: 409-4012
  • Peer Reviewed
• [Journal Article] Thermal Denaturation and Renaturation of &#947;-Glutamyltranspeptidase of <i>Escherichia coli</i> (2013)
  • Author(s): V. H. Thao
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 2 Pages: 409-412
  • DOI: 10.1271/bbb.120780
  • NAID: 10031164753
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Establishment of a Bacterial Expression System and Immunoassay Platform for the Major Capsid Protein of HcRNAV, a Dinoflagellate-Infecting RNA Virus (2012)
  • Author(s): K. Wada
  • Journal Title: Microbes and Environments Volume: 27 Issue: 4 Pages: 483-489
  • DOI: 10.1264/jsme2.ME12046
  • NAID: 10031125948
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Journal Article] large-scale overexpression methodof deriving carbon monoxide dehydrogenaseII from the thermophilic bacteriumCarboxdothermus hydrpgenformans (2011)
  • Author(s): Inoue, T., Yoshida, Y., Wada, K.,Daifuku, T., Fukuyama, K., Sako, Y. Asimple
  • Journal Title: Biosci.Biotech. Biochem. Volume: 75 Pages: 1382-1394
  • Peer Reviewed
• [Journal Article] Mapping of protein-proteininteraction sites in the plant-type[2Fe-2S] ferredoxin (2011)
  • Author(s): Kameda, H., Hirabayashi, K., Wada,K.,Fukuyama K
  • Journal Title: PLoS ONE
  • Peer Reviewed
• [Journal Article] Mapping of protein-protein interaction sites in the plant-type [2Fe-2S] ferredoxin (2011)
  • Author(s): Kameda, H., Hirabayashi, K., Wada, K., Fukuyama K.
  • Journal Title: PLoS ONE Volume: 該当なし Issue: 7 Pages: e21947-e21947
  • DOI: 10.1371/journal.pone.0021947
  • Peer Reviewed
• [Journal Article] A Simple, Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium Carboxydothermus hydrogenoformans (2011)
  • Author(s): Inoue, T., Yoshida, T, Wada, K., Daifuku, T., Fukuyama, K., Sako, Y.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 7 Pages: 1392-1394
  • DOI: 10.1271/bbb.110159
  • NAID: 10029329599
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] 有害渦鞭毛藻ヘテロカプサに感染する HcRNAV を検出する抗体の開発と実用性の検証 (2012)
  • Author(s): 木村 圭、和田 啓、長谷川昭文、福山恵一、外丸裕司、長崎慶三
  • Organizer: 成 24 年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2012-03-29
• [Presentation] Structural biology of the SUFmachinery involved in de novo iron-sulfurcluster biosynthesis. (2012)
  • Author(s): Wada K
  • Organizer: 4th FEBS SpecialMeeting, ATP-Binding Cassette Proteins:From Multidrug Registance to GeneticDiseases
  • Place of Presentation: Hotel Grauer Bar(オーストリア インスブルック)
  • Year and Date: 2012-03-04
• [Presentation] Preliminary X-raydiffraction analysis and establishment ofthe immunoassay platform of thedinoflagellate-infecting marine virusHcRNAV. (2012)
  • Author(s): Tanaka, E, Hasegawa, A., Kimura, K.,Nakaniwa, T., Tomaru, Y, Fukuyama, K.,Nagasaki, K., Wada, K
  • Organizer: The Asian Crystallographic Society and theSociety of Crystallographers in Australiaand New Zealand
  • Place of Presentation: Adelaide Convention Centre(オーストラリア アデレード)
• [Presentation] 有害赤潮原因藻に感染するウイルスHcRNAV粒子の結晶学的研究および特異的抗体の性状解析 (2012)
  • Author(s): 長谷川昭文、木村圭、外丸裕司、長崎慶三、福山恵一、和田啓
  • Organizer: 日本結晶学会平成24年度年会
  • Place of Presentation: 東北大学片平キャンパス
• [Presentation] 有害赤潮原因藻に感染するウイルスHcRNAVの精製と結晶化 (2012)
  • Author(s): 長谷川昭文、木村圭、外丸裕司、福山恵一、長崎慶三、和田啓
  • Organizer: 第12回蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 有害赤潮原因藻に感染するウイルスHcRNAVの精製と結晶化 (2012)
  • Author(s): 和田啓
  • Organizer: 第12回蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場（愛知）
• [Presentation] 有害赤潮原因藻に感染するウイルスHcRNAV粒子の結晶学的研究および特異的抗体の性状解析 (2012)
  • Author(s): 和田啓
  • Organizer: 日本結晶学会平成24年度年会
  • Place of Presentation: 東北大学片平キャンパス（宮城県）
• [Presentation] Preliminary X-ray diffraction analysis and establishment of the immunoassay platform of the dinoflagellate-infecting marine virus HcRNAV (2012)
  • Author(s): Eri Tanaka
  • Organizer: AsCA 12/CRYSTAL28
  • Place of Presentation: Adelaide Convention Centre（オーストラリア アデレード）
• [Presentation] 有害赤潮原因藻に感染するウイルスHcRNAV粒子の結晶学的研究 (2011)
  • Author(s): 長谷川昭文、田中悠喜、外丸裕司、長崎慶三、福山恵一、和田啓
  • Organizer: 第61回日本結晶学会年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-11-24
• [Presentation] フェレドキシン依存的ビリン還元酵素PcyA変異体-ビリベルジン複合体の構造解析 (2011)
  • Author(s): 和田啓、萩原義徳、入川鉄平、油谷裕子、福山恵一
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-22
• [Presentation] Oneresidue substitution in PcyA leads tounexpected changes in tetrapyrrolesubstrate binding (2011)
  • Author(s): Wada K., Hagiwara Y., Fukuyama, K
  • Organizer: XXII Congress andGeneral Assembly International Union ofCrystallography
  • Place of Presentation: Palacio Municipal de Madrid(スペイン マドリード)
  • Year and Date: 2011-08-27
• [Presentation] Crystallographicanalyses of the ISC proteins involved in denovo Fe-S cluster biogenesis (2011)
  • Author(s): Hirabayashi, K., Takahashi, Y.,Fukuyama, K., Wada, K
  • Organizer: XXIICongress and General AssemblyInternational Union of Crystallography
  • Place of Presentation: Palacio Municipal deMadrid(スペイン マドリード)
  • Year and Date: 2011-08-27
• [Presentation] 超好熱菌Aquifex aeolicusの鉄硫黄クラスター生合成に関わる脱硫黄酵素IscSの構造解析 (2011)
  • Author(s): 平林佳、岩永朋子、高橋康弘、福山恵一、和田啓
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク
  • Year and Date: 2011-06-07
• [Presentation] 超好熱菌Aquifex aeolicusの鉄硫黄クラスター生合成に関わる脱硫黄酵素IscSの構造解析
  • Author(s): 平林佳、岩永朋子、高橋康弘、福山恵一、和田啓
  • Organizer: 第１１回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク（大阪府）
• [Presentation] 有害赤潮原因藻に感染するウイルスHcRNAV粒子の結晶学的研究
  • Author(s): 長谷川昭文、田中悠喜、外丸裕司、長崎慶三、福山恵一、和田啓
  • Organizer: 第61回日本結晶学会年会
  • Place of Presentation: 北海道大学（北海道）
• [Presentation] フェレドキシン依存的ビリン還元酵素PcyA変異体-ビリベルジン複合体の構造解析
  • Author(s): 和田啓、萩原義徳、入川鉄平、油谷裕子、福山恵一
  • Organizer: 第８４回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Crystallographic analyses of the ISC proteins involved in de novo Fe-S cluster biogenesis
  • Author(s): 平林佳、高橋康弘、福山恵一、和田啓
  • Organizer: XXII Congress and General Assembly International Union of Crystallography
  • Place of Presentation: Palacio Municipal de Madrid(スペイン マドリード)
• [Presentation] One residue substitution in PcyA leads to unexpected changes in tetrapyrrole substrate binding
  • Author(s): 和田啓、萩原義徳、福山恵一
  • Organizer: XXII Congress and General Assembly International Union of Crystallography
  • Place of Presentation: Palacio Municipal de Madrid(スペイン マドリード)
• [Presentation] Structural biology of the SUF machinery involved in de novo iron-sulfur cluster biosynthesis
  • Author(s): 和田啓
  • Organizer: 4th FEBS Special Meeting, ATP-Binding Cassette Proteins: From Multidrug Registance to Genetic Diseases(招待講演)
  • Place of Presentation: Hotel Grauer Bar (オーストリア インスブルック)
• [Presentation] 有害渦鞭毛藻ヘテロカプサに感染する HcRNAV を検出する抗体の開発と実用性の検証
  • Author(s): 木村 圭、和田 啓、長谷川昭文、福山恵一、外丸裕司、長崎慶三
  • Organizer: 平成 24 年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス（東京都）
• [Remarks]
  • URL: http://www.cc.miyazaki-u.ac.jp/keiwada/index.htm
• [Remarks] 宮崎大学医学系物質科学分野和田研究室
  • URL: http://www.cc.miyazaki-u.ac.jp/keiwada/index.htm
• [Remarks]
  • URL: http://www.bio.sci.osaka-u.ac.jp/%7Ekeiwada/index.htm