Research Project
Grant-in-Aid for Challenging Exploratory Research
We previously showed that E. coli PriA protein, in collaboration with RecG, specifically binds and can stabilize an arrested replication fork. In this proposal we attempted to stabilize stalled replication forks in eukaryotic cells by utilizing these two proteins. We first developed an antibody against PriA and showed that stalled replication forks can be detected by ChIP-chip analyses. In response to fork arrest treatment, PriA bound to oriC in the wild-type cells, whereas it bound to the terC region under the condition where oriC is not functional. We then expressed PriA in fission yeast cells, examined its binding to chromatin. PriA was enriched near the replication origins. We are now expressing PriA in various mutant cells, and will examine if PriA can stabilize the fork by measuring the length of SSB coated single-strand.
All 2014 2013 2012 2011 Other
All Journal Article (44 results) (of which Peer Reviewed: 42 results, Open Access: 10 results, Acknowledgement Compliant: 9 results) Presentation (31 results) (of which Invited: 27 results) Book (1 results) Remarks (1 results)
Mol. Cell
Volume: 55 Issue: 1 Pages: 123-137
10.1016/j.molcel.2014.04.031
Methods in Molecular Biology
Volume: 1170 Pages: 279-293
10.1007/978-1-4939-0888-2_13
Cell Cycle
Volume: 13 Pages: 882-883
Seminars in Cell & Developmental Biology,
Volume: 30 Pages: 110-120
10.1016/j.semcdb.2014.04.014
Cell Cycle May
Volume: 19
Volume: in press
Volume: 13 Issue: 6 Pages: 882-883
10.4161/cc.28216
Seminars in Cell & Developmental Biology
J. Mol. Biol.
Volume: 425 Issue: 23 Pages: 4663-4672
10.1016/j.jmb.2013.03.039
Biochemical Society Transactions
Volume: 41 Pages: 1712-1719
Genes and Development
Volume: 27 Pages: 2459-2472
J. Biol. Chem.
Volume: 288 Issue: 18 Pages: 12742-12752
10.1074/jbc.m112.398073
Nucleic Acids Res.
Volume: 41 Issue: 18 Pages: 8475-8488
10.1093/nar/gkt623
Br. J. Cancer.
Volume: 109 Issue: 12 Pages: 3042
10.1038/bjc.2013.532
Volume: 27 Issue: 22 Pages: 2459-72
10.1101/gad.224568.113
Trends in Genetics
Volume: 29 Issue: 8 Pages: 449-460
10.1016/j.tig.2013.05.001
PLoS One
Volume: 8 Issue: 8 Pages: e72408-e72408
10.1371/journal.pone.0072408
Volume: In press
Genes & Dev
Volume: 25 Issue: 2 Pages: 137-150
10.1101/gad.178491.111
EMBO J.
Volume: 31 Issue: 18 Pages: 3167-3177
10.1038/emboj.2012.180
Mol. Biol. Cell.
Volume: 23 Issue: 10 Pages: 1943-1954
10.1091/mbc.e11-10-0829
The Journal of biological chemistry
Volume: 287 Issue: 28 Pages: 23977-23994
10.1074/jbc.m112.368456
Volume: 7
Methods
Volume: 57 Issue: 2 Pages: 214-221
10.1016/j.ymeth.2012.06.016
Molecular Cell
Volume: 14 Issue: 5 Pages: 722-733
10.1016/j.molcel.2012.06.023
Volume: 8
UCSD-Nature Molecule Pages
Volume: (Published online) Pages: 31-31
10.6072/H0.MP.A003137.01
The Encyclopedia of Signaling Molecules Springer Reference and Database Publishing
J Biol Chem
Volume: Nov 23;287(48) Issue: 48 Pages: 40256-40265
10.1074/jbc.m112.353805
Volume: 7 Issue: 8 Pages: 214-221
10.1371/journal.pone.0042375
Volume: 7 Issue: 5 Pages: e36372-e36372
10.1371/journal.pone.0036372
細胞工学
Volume: 31 Pages: 460-462
実験医学、Current Topics
Volume: 30 Pages: 2974-2978
Genes Dev.
Volume: 26 Pages: 137-150
J. Biochem
Volume: 149 Pages: 629-631
10030585512
Volume: 286 Issue: 26 Pages: 23031-23043
10.1074/jbc.m111.243311
Mol. Cell. Biol.
Volume: 31 Issue: 12 Pages: 2380-2389
10.1128/mcb.01239-10
Genes to Cells
Volume: 16 Pages: 842-856
J. Cell Biol.
Volume: 195 Issue: 3 Pages: 387-401
10.1083/jcb.201107025
J Cell Biol.
Volume: 31 Pages: 387-401
Mol Cell Biol.
Volume: 31 Pages: 2380-2391
http://www.igakuken.or.jp/genome/