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Stabilization of stalled replication forks by fork-binding proteins

Research Project

Project/Area Number 23657081
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

MASAI Hisao  公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 基盤技術研究センター長 (40229349)

Co-Investigator(Kenkyū-buntansha) TANAKA Taku  公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (80425686)
Co-Investigator(Renkei-kenkyūsha) MATUMOTO Seiji  公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (40190532)
KAKUSHO Naoko  公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (30599593)
KANOH Yutaka  公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (90450593)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsPriAタンパク質 / 複製フォーク / 複製ストレス / RecGタンパク質 / チェックポイント応答 / 染色体脆弱部位 / DNA結合タンパク質 / 染色体不安定性症候群 / 複製複合体 / DNA複製停止 / ゲノム不安定性
Research Abstract

We previously showed that E. coli PriA protein, in collaboration with RecG, specifically binds and can stabilize an arrested replication fork. In this proposal we attempted to stabilize stalled replication forks in eukaryotic cells by utilizing these two proteins. We first developed an antibody against PriA and showed that stalled replication forks can be detected by ChIP-chip analyses. In response to fork arrest treatment, PriA bound to oriC in the wild-type cells, whereas it bound to the terC region under the condition where oriC is not functional. We then expressed PriA in fission yeast cells, examined its binding to chromatin. PriA was enriched near the replication origins. We are now expressing PriA in various mutant cells, and will examine if PriA can stabilize the fork by measuring the length of SSB coated single-strand.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (77 results)

All 2014 2013 2012 2011 Other

All Journal Article (44 results) (of which Peer Reviewed: 42 results,  Open Access: 10 results,  Acknowledgement Compliant: 9 results) Presentation (31 results) (of which Invited: 27 results) Book (1 results) Remarks (1 results)

  • [Journal Article] NCOA4 Transcriptional Coactivator Inhibits Activation of DNA Replication Origins.2014

    • Author(s)
      Bellelli, R., Castellone, M.D., Guida, T., Limongello, R., Dathan, N.A., Merolla, F., Cirafici, A.M., Affuso, A., Masai, H., Costanzo, V., Grieco, D., Fusco, A., Santoro, M., and Carlomagno, F.
    • Journal Title

      Mol. Cell

      Volume: 55 Issue: 1 Pages: 123-137

    • DOI

      10.1016/j.molcel.2014.04.031

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cell cycle synchronization and flow cytometry analysis of mammalian cell.2014

    • Author(s)
      Yoshizawa-Sugata, N. and Masai, H.
    • Journal Title

      Methods in Molecular Biology

      Volume: 1170 Pages: 279-293

    • DOI

      10.1007/978-1-4939-0888-2_13

    • ISBN
      9781493908875, 9781493908882
    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] News and Views "ATM in prevention of genomic instability"2014

    • Author(s)
      Hisao Masai
    • Journal Title

      Cell Cycle

      Volume: 13 Pages: 882-883

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Temporal and spatial regulation of eukaryotic DNA replication: from regulated initiation to genome-scale timing program.2014

    • Author(s)
      Renard-Guillet, C., Kanoh, Y., Shirahige, K., and Masai, H.
    • Journal Title

      Seminars in Cell & Developmental Biology,

      Volume: 30 Pages: 110-120

    • DOI

      10.1016/j.semcdb.2014.04.014

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulation and roles of Cdc7 kinase under replication stress2014

    • Author(s)
      Yamada, M., Masai, H., and Bartek, J
    • Journal Title

      Cell Cycle May

      Volume: 19

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] "Cell cycle synchronization and flow cytometry analysis of mammalian cell."2014

    • Author(s)
      Yoshizawa-Sugata, N. and Masai, H
    • Journal Title

      Methods in Molecular Biology

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ATM in prevention of genomic instability.2014

    • Author(s)
      Hisao Masai
    • Journal Title

      Cell Cycle

      Volume: 13 Issue: 6 Pages: 882-883

    • DOI

      10.4161/cc.28216

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] "Temporal and spatial regulation of eukaryotic DNA replication: From regulated initiation to genome-scale timing program."2014

    • Author(s)
      Claire Renard-Guillet, Yutaka Kanoh, Katsuhiko Shirahige, and Hisao Masai
    • Journal Title

      Seminars in Cell & Developmental Biology

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A personal reflection on the Replicon Theory: from R1 plasmid to replication timing regulation in human cells2013

    • Author(s)
      Masai, H.
    • Journal Title

      J. Mol. Biol.

      Volume: 425 Issue: 23 Pages: 4663-4672

    • DOI

      10.1016/j.jmb.2013.03.039

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulation of chromosome dynamics by Hsk1 kinase2013

    • Author(s)
      Matsumoto, S. and Masai, H
    • Journal Title

      Biochemical Society Transactions

      Volume: 41 Pages: 1712-1719

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ATR-Chk1-APC/CCdh1-dependent stabilization of Cdc7-ASK(Dbf4) kinase complex is required for DNA damage bypass under replication stress2013

    • Author(s)
      Yamada, M., Watanabe, K., Mistrik, M., Mailand, N., Lee, M-H., Masai, H., Lukas, J. and Bartek, B
    • Journal Title

      Genes and Development

      Volume: 27 Pages: 2459-2472

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Human Tim/Tipin Complex Directly Interacts with DNA Polymerase {epsilon} and Stimulates its Synthetic Activity.2013

    • Author(s)
      Aria, V., De Felice, M., Di Perna, R., Uno, S., Masai, H., Syvaoja, J.E., van Loon, B., Hubscher, U. and Pisani, F.M.
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Issue: 18 Pages: 12742-12752

    • DOI

      10.1074/jbc.m112.398073

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Analysis of epigenetic stability and conversions in Saccharomyces cerevisiae reveals a novel role of CAF-I in position-effect variegation.2013

    • Author(s)
      Jeffery, D.C., Wyse, B.A., Rehman, M.A., Brown, G.W., You, Z., Oshidari, R., Masai, H. and Yankulov, K.Y.
    • Journal Title

      Nucleic Acids Res.

      Volume: 41 Issue: 18 Pages: 8475-8488

    • DOI

      10.1093/nar/gkt623

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Role of multifunctional transcription factor TFII-I and putative tumour suppressor DBC1 in cell cycle and DNA double strand damage repair2013

    • Author(s)
      Tanikawa, M., Wada-Hiraike, O., Yoshizawa-Sugata, N., Shirane A, Hirano M, Hiraike H, Miyamoto, Y., Sone, K., Ikeda, Y., Kashiyama, T., Oda K, Kawana K, Katakura Y, Yano T, Masai, H., Roy AL, Osuga, Y. and Fujii, T.
    • Journal Title

      Br. J. Cancer.

      Volume: 109 Issue: 12 Pages: 3042

    • DOI

      10.1038/bjc.2013.532

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ATR-Chk1-APC/CCdh1-dependent stabilization of Cdc7-ASK(Dbf4) kinase complex is required for DNA damage bypass under replication stress.2013

    • Author(s)
      Yamada, M., Watanabe, K., Mistrik, M., Mailand, N., Lee, M-H., Masai, H., Lukas, J. and Bartek, B.
    • Journal Title

      Genes and Development

      Volume: 27 Issue: 22 Pages: 2459-72

    • DOI

      10.1101/gad.224568.113

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Replication timing regulation of eukaryotic replicons: Rif1 as a global regulator of replication timing.2013

    • Author(s)
      Yamazaki, S. Hayano, M. and Masai, H.
    • Journal Title

      Trends in Genetics

      Volume: 29 Issue: 8 Pages: 449-460

    • DOI

      10.1016/j.tig.2013.05.001

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] "MCM helicase interacts with primase and stimulates its priming activity."2013

    • Author(s)
      You, Z., De Falco, S., Pisani, F.M. and Masai, H
    • Journal Title

      PLoS One

      Volume: 8 Issue: 8 Pages: e72408-e72408

    • DOI

      10.1371/journal.pone.0072408

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] "Regulation of chromosome dynamics by Hsk1 kinase."2013

    • Author(s)
      Matsumoto, S. and Masai, H
    • Journal Title

      Biochemical Society Transactions

      Volume: 41 Pages: 1712-1719

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Replication timing regulation of eukaryotic replicons: Rif1 as a global regulator of replication timing.2013

    • Author(s)
      Yamazaki, S. Hayano, M. and Masai, H.
    • Journal Title

      Trends in Genetics

      Volume: In press

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rif1 is a global regulator of timing of replication origin firing in fission yeast2012

    • Author(s)
      Hayano M, Kanoh Y, Matsumoto S, Renard-Guillet C, Shirahige K, Masai H
    • Journal Title

      Genes & Dev

      Volume: 25 Issue: 2 Pages: 137-150

    • DOI

      10.1101/gad.178491.111

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rif1 protein is a key regulator of the genome-wide DNA replication timing in human cells.2012

    • Author(s)
      Yamazaki, S., Ishii, A., Kanoh, Y., Oda, M., Nishito, Y. and Masai, H.
    • Journal Title

      EMBO J.

      Volume: 31 Issue: 18 Pages: 3167-3177

    • DOI

      10.1038/emboj.2012.180

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] c-Jun N-terminal Kinase (JNK)-Mediated Rad18 Phosphorylation Facilitates Polη Recruitment to Stalled Replication Forks.2012

    • Author(s)
      Barkley, L.R., Palle, K., Durando, M., Day, T.A., Gurkar, A., Kakusho, N., Li, J., Masai, H., Vaziri, C.
    • Journal Title

      Mol. Biol. Cell.

      Volume: 23 Issue: 10 Pages: 1943-1954

    • DOI

      10.1091/mbc.e11-10-0829

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Epstein-Barr nuclear antigen 1 (EBNA1)-dependent recruitment of origin recognition complex(Orc) on oriP of Epstein-Barr virus with purified proteins:stimulation by Cdc6 through its direct interaction with EBNA1.2012

    • Author(s)
      Moriyama K
    • Journal Title

      The Journal of biological chemistry

      Volume: 287 Issue: 28 Pages: 23977-23994

    • DOI

      10.1074/jbc.m112.368456

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanism of cancer cell death induced by depletion of an essential replication regulator2012

    • Author(s)
      Ito, S., Ishii, A., Kakusho, N., Taniyama, C., Yamazaki, S., Sakaue-Sawano, A., Miyawaki, A., and Masai, H
    • Journal Title

      PLoS One

      Volume: 7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification of replication factors using insect and mammalian cell expression systems.2012

    • Author(s)
      Uno, S., You, Z., and Masai, H.
    • Journal Title

      Methods

      Volume: 57 Issue: 2 Pages: 214-221

    • DOI

      10.1016/j.ymeth.2012.06.016

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of DNA replication timing on human chromosome by a cell-type specific DNA binding protein SATB12012

    • Author(s)
      Oda, M., Kanoh, Y., Watanabe, Y., and Masai, H
    • Journal Title

      PLoS One

      Volume: 7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A central coupler for recombination initiation linking chromosome architecture to S phase checkpoint.2012

    • Author(s)
      三好知一郎
    • Journal Title

      Molecular Cell

      Volume: 14 Issue: 5 Pages: 722-733

    • DOI

      10.1016/j.molcel.2012.06.023

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] MCM helicase interacts with primase and stimulates its priming activity2012

    • Author(s)
      You, Z., De Falco, S., Pisani, F.M. and Masai, H
    • Journal Title

      PLoS One

      Volume: 8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cdc7L12012

    • Author(s)
      Toh G-T. and Masai, H
    • Journal Title

      UCSD-Nature Molecule Pages

      Volume: (Published online) Pages: 31-31

    • DOI

      10.6072/H0.MP.A003137.01

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cdc72012

    • Author(s)
      Masai, H
    • Journal Title

      The Encyclopedia of Signaling Molecules Springer Reference and Database Publishing

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dbf42012

    • Author(s)
      Masai, H
    • Journal Title

      The Encyclopedia of Signaling Molecules Springer Reference and Database Publishing

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of DNA damage-induced apoptosis through Cdc7-mediated stabilization of Tob.2012

    • Author(s)
      Suzuki T
    • Journal Title

      J Biol Chem

      Volume: Nov 23;287(48) Issue: 48 Pages: 40256-40265

    • DOI

      10.1074/jbc.m112.353805

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of DNA replication timing on human chromosome by a cell-type specific DNA binding protein SATB1.2012

    • Author(s)
      Oda, M., Kanoh, Y., Watanabe, Y., and Masai, H.
    • Journal Title

      PLoS One

      Volume: 7 Issue: 8 Pages: 214-221

    • DOI

      10.1371/journal.pone.0042375

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanism of cancer cell death induced by depletion of an essential replication regulator.2012

    • Author(s)
      Ito, S., Ishii, A., Kakusho, N., Taniyama, C., Yamazaki, S., Sakaue-Sawano, A., Miyawaki, A., and Masai, H.
    • Journal Title

      PLoS One

      Volume: 7 Issue: 5 Pages: e36372-e36372

    • DOI

      10.1371/journal.pone.0036372

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] テロメア結合因子Rif1は、染色体複タイミングを決定する2012

    • Author(s)
      早野元詞、加納豊、松本清治、正井久雄
    • Journal Title

      細胞工学

      Volume: 31 Pages: 460-462

    • Related Report
      2012 Research-status Report
  • [Journal Article] Rif1タンパク質はヒトゲノム複製タイミングドメインを決定する。2012

    • Author(s)
      山崎 聡志、正井 久雄
    • Journal Title

      実験医学、Current Topics

      Volume: 30 Pages: 2974-2978

    • Related Report
      2012 Research-status Report
  • [Journal Article] Rif1 is a global regulator of timing of replication origin firing in fission yeast.2012

    • Author(s)
      Hayano M, Kanoh Y, Matsumoto S, Renard-Guillet C, Shirahige K, Masai H.
    • Journal Title

      Genes Dev.

      Volume: 26 Pages: 137-150

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] RecQL4 : a helicase linking formation and maintenance of a replication fork2011

    • Author(s)
      Masai, H
    • Journal Title

      J. Biochem

      Volume: 149 Pages: 629-631

    • NAID

      10030585512

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanism of activation of human Cdc7 kinase: Bipartite interaction with Dbf4/ASK stimulates ATP binding and substrate recognition.2011

    • Author(s)
      Kitamura, R., Fukatsu, R., Kakusho, N., Cho, Y-S., Taniyama, C., Yamazaki, S., Toh, G-T., Yanagi, K., Arai, N., Chang, H-J. and Masai, H.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Issue: 26 Pages: 23031-23043

    • DOI

      10.1074/jbc.m111.243311

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pre-firing binding of Mrc1 defines the early-firing origins which are selectively hyper-activated upon loss of fork stabilizing factors in fission yeast.2011

    • Author(s)
      Hayano, M., Kanoh, Y., Matsumoto, S., Kakusho, N. and Masai, H.
    • Journal Title

      Mol. Cell. Biol.

      Volume: 31 Issue: 12 Pages: 2380-2389

    • DOI

      10.1128/mcb.01239-10

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient expression and purification of human replication fork-stabilizing factor, Claspin, from mammalian cells : DNA binding activity and novel protein interactions2011

    • Author(s)
      Uno, S and Masai, H
    • Journal Title

      Genes to Cells

      Volume: 16 Pages: 842-856

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.2011

    • Author(s)
      Matsumoto, S., Hayano, M., Kanoh. Y. and Masai, H.
    • Journal Title

      J. Cell Biol.

      Volume: 195 Issue: 3 Pages: 387-401

    • DOI

      10.1083/jcb.201107025

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.2011

    • Author(s)
      Matsumoto S, Hayano M, Kanoh Y, Masai H.
    • Journal Title

      J Cell Biol.

      Volume: 31 Pages: 387-401

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mrc1 marks early-firing origins and coordinates timing and efficiency of initiation in fission yeast.2011

    • Author(s)
      Hayano M, Kanoh Y, Matsumoto S, Masai H.
    • Journal Title

      Mol Cell Biol.

      Volume: 31 Pages: 2380-2391

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 染色体DNA 複製プログラムの制御機構と起源・進化2014

    • Author(s)
      正井 久雄, 田中 卓, 加納 豊, 山崎 聡志, 松本 清治, 吉沢 直子, 松嶋 夢叶
    • Organizer
      The 2nd Symposium of Cell Cycle Control and Cell Fate (第2回 細胞周期制御と細胞運命シンポジウム)
    • Place of Presentation
      浜松
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] "染色体DNA複製プログラムの制御機構と起源・進化"2014

    • Author(s)
      正井 久雄、田中 卓、加納 豊、山崎 聡志、松本 清治、吉沢 直子、松嶋 夢叶
    • Organizer
      The 2nd Symposium of Cell Cycle Control and Cell Fate (第2回 細胞周期制御と細胞運命 シンポジウム)
    • Place of Presentation
      浜松
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Cell cycle and chromatin regulators in stem cell regulation2013

    • Author(s)
      Hisao Masai
    • Organizer
      The Gene and Immnuotherapy conference
    • Place of Presentation
      Ho chi Min City, Vietnam
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Invited
  • [Presentation] "A personal reflection on the Replicon Theory : from R1 plasmid to replication timing regulation in human cells" "Symposium : Half a Century With Replicon Theory for Genome Stability and Instability"2013

    • Author(s)
      Hisao Masai
    • Organizer
      The 50th anniversary of the replicon theory
    • Place of Presentation
      Pasteur Institute, Paris, France
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Control of origin firing timing in fission yeast cells2013

    • Author(s)
      Hisao Masai
    • Organizer
      EMBO Conference "Pombe 2013 7th International Fission Yeast Meeting"
    • Place of Presentation
      London UK
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] "Introduction" Symposium2013

    • Author(s)
      Hisao Masai
    • Organizer
      86th Annual Meeting of the Japanese Biochemical Society
    • Place of Presentation
      Fukuoka, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Chromatin architecture that regulates replication timing of eukaryotic chromosomes2013

    • Author(s)
      Hisao Masai, Yutaka Kanoh, Satoshi Yamazaki, Motoshi Hayano, Naoko Yoshizawa, Kenji Moriyama, Yumeka Matsushima, Naoko Kakusho, Rino Fukatsu
    • Organizer
      第86回日本生化学会大会事務局 インターナショナルセッション『Assembly and architecture of protein complexes regulating inheritance and stable maintenance of genome』
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] イントロダクション2013

    • Author(s)
      正井 久雄
    • Organizer
      第36回(2013年)日本分子生物学会年会, ワークショップ 『レプリコン仮説50周年 : 染色体複製装置の形成とその活性の時空間制御』オーガナイザー
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Renard-Guillet Claire "Rif1 : a potential global regulator of DNA replication, DNA repair, and recombination"2013

    • Author(s)
      正井 久雄, 山崎 聡志, Lai Mongsing, Foiani Marco, 吉沢 直子, 加納 豊, 松本 清治, 森山賢治, 覺正 直子, 深津 理乃, 松嶋 夢叶, 河野 暢明
    • Organizer
      第36回(2013年)日本分子生物学会年会, ワークショップ 『DNA 二重鎖切断のend-resection と修復機構の選択・制御』
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] "Control of origin firing timing in fission yeast cells"2013

    • Author(s)
      Hisao Masai
    • Organizer
      EMBO Conference "Pombe 2013: 7th International Fission Yeast Meeting"
    • Place of Presentation
      London UK
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Introductory Remarks2013

    • Author(s)
      Hisao Masai
    • Organizer
      86th Annual Meeting of the Japanese Biochemical Society
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] “Chromatin architecture that regulates replication timing of eukaryotic chromosomes.“2013

    • Author(s)
      Hisao Masai, Yutaka Kanoh, Satoshi Yamazaki, Motoshi Hayano, Naoko Yoshizawa, Kenji Moriyama, Yumeka Matsushima, Naoko Kakusho, and Rino Fukatsu
    • Organizer
      第86回日本生化学会大会事務局 インターナショナルセッション「Assembly and architecture of protein complexes regulating inheritance and stable maintenance of genome」
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] レプリコン仮説50周年2013

    • Author(s)
      正井 久雄
    • Organizer
      第36回(2013年)日本分子生物学会年会,ワークショップ 「レプリコン仮説50周年:染色体複製装置の形成とその活性の時空間制御」
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] "Rif1: a potential global regulator of DNA replication, DNA repair, and recombination."2013

    • Author(s)
      正井 久雄、山崎 聡志、Lai Mongsing、Foiani Marco、吉沢 直子、加納 豊、松本 清治、森山賢治、覺正 直子、深津 理乃、松嶋 夢叶、河野 暢明、Renard-Guillet Claire
    • Organizer
      第36回(2013年)日本分子生物学会年会,ワークショップ 「DNA二重鎖切断のend-resectionと修復機構の選択・制御」
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] A personal reflection on the Replicon Theory: from R1 plasmid to replication timing regulation in human cells” Symposium “Half a Century With Replicon Theory for Genome Stability and Instability2013

    • Author(s)
      Hisao Masai
    • Organizer
      The 50th anniversary of the replicon theory
    • Place of Presentation
      Pasteur Institute, Paris, France
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Regulation of replication program in fission yeast and human cells2012

    • Author(s)
      Hisao Masai
    • Organizer
      1st Igakuken Symposium on"Regulation of Chromosome Cycle"
    • Place of Presentation
      Igakuken, Tokyo
    • Year and Date
      2012-11-29
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Regulation of replication fork in maintenance of genomic integrity2012

    • Author(s)
      Hisao Masai
    • Organizer
      (2012 Arthur Kornberg Memorial Award Lecture) 15th A-IMBN Annual Conference
    • Place of Presentation
      Shanghai
    • Year and Date
      2012-10-23
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Regulation of replication program in fission yeast and human cell2012

    • Author(s)
      Hisao Masai
    • Organizer
      Department of Biological Science
    • Place of Presentation
      Florida State University
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Cell cycle regulation of mouse embryonic stem cells : E2F- and Emi1-mediated positive feedback regulation2012

    • Author(s)
      Hisao Masai, Hiroko Fujii, Naoko Kakusho, Sayuri Ito, Satoshi Yamazaki, and Naoko Yoshizawa
    • Organizer
      4th Southeast Stem Cell Consortium Workshop at Florida State University
    • Place of Presentation
      Tallahassee, Florida
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Regulation of replication program in fission yeast and human cells2012

    • Author(s)
      Hisao Masai, Satoshi Yamazaki, Yutaka Kanoh, Motoshi Hayano, Seiji Matsumoto, Naoko Yoshizawa, Rino Fukatsu, Naoko Kakusho, and Michie Shimmoto
    • Organizer
      8th 3R meeting
    • Place of Presentation
      Awajishima
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 解体再構成的アプローチから合成生物学・生命現象シミュレーションへ : DNA複製をモデルとして2012

    • Author(s)
      正井 久雄
    • Organizer
      第85回日本生化学会大会 シンポジウム
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 解体再構成的アプローチから合成生物学•生命現象シミュレーションへ:DNA複製をモデルとして2012

    • Author(s)
      正井久雄
    • Organizer
      第85回日本生化学会大会 シンポジウム
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Regulation of replication fork in maintenance of genomic integrity2012

    • Author(s)
      Hisao Masai
    • Organizer
      15th A-IMBN Annual Conference
    • Place of Presentation
      Shanghai
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Cell cycle regulation of mouse embryonic stem cells: E2F- and Emi1-mediated positive feedback regulation.2012

    • Author(s)
      Shanghai
    • Organizer
      4th Southeast Stem Cell Consortium Workshop at Florida State University
    • Place of Presentation
      Tallahassee, Florida
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Regulation of the replication program in fission yeast and human cells2011

    • Author(s)
      Hisao Masai
    • Organizer
      Seminar
    • Place of Presentation
      Hong Kong University of Science and Technology
    • Year and Date
      2011-11-25
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Regulation of replication program in fission yeast and human cells2011

    • Author(s)
      Hisao Masai, Satoshi Yamazaki, Yutaka Kanoh, Motoshi Hayano, Seiji Matsumoto, Naoko Kakusho
    • Organizer
      Cold Spring Harbor Laboratory Meeting Eukaryotic DNA Replication & Genome Maintenance
    • Place of Presentation
      New York, USA
    • Year and Date
      2011-09-06
    • Related Report
      2013 Final Research Report
  • [Presentation] DNA 複製制御因子を標的とした制がん戦略 : Cdc7 キナーゼ阻害によるがん細胞死誘導2011

    • Author(s)
      正井久雄
    • Organizer
      第9回日本臨床腫瘍学会/日本癌学会/分子標的治療学会 合同シンポジウム"基礎から学ぶ次世代の分子標的治療薬と基盤技術"
    • Place of Presentation
      東京
    • Year and Date
      2011-07-21
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Regulation of replication program in fission yeast2011

    • Author(s)
      Hisao Masai, Seiji Matsumoto, Motoshi Hayano, Yutaka Kanoh, Michie Shimmoto, Naoko Kakusho, and Satomi Kudo
    • Organizer
      The 6th International Fission Yeast Meeting
    • Place of Presentation
      Boston, USA
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Rif1 is a global regulator of site selection and timing of replication origin firing2011

    • Author(s)
      Motoshi Hayano, Yutaka Kanoh, Seiji Matsumoto and Hisao Masai
    • Organizer
      The 6th International Fission Yeast Meeting
    • Place of Presentation
      Boston, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] "Regulation of replication program in fission yeast and human cells."2011

    • Author(s)
      Hisao Masai, Satoshi Yamazaki, Yutaka Kanoh, Motoshi Hayano, Seiji Matsumoto, Masako Oda, Naoko Kakusho, Rino Fukatsu, Michie Shimmoto and Naoko Yoshizawa
    • Organizer
      日本分子生物学会総会 シンポジウム(招待講演)
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] DNA複製制御因子を標的とした制がん戦略:Cdc7キナーゼ阻害によるがん細胞死誘導2011

    • Author(s)
      正井久雄
    • Organizer
      第9回 日本臨床腫瘍学会/日本癌学会/分子標的治療学会 合同シンポジウム "基礎から学ぶ次世代の分子標的治療薬と基盤技術"(招待講演)
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Book] 細胞工学2012

    • Author(s)
      早野元詞、加納豊、松本清治、正井久雄
    • Total Pages
      490
    • Publisher
      秀潤社
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.igakuken.or.jp/genome/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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