Roles of degradation products generated by the proteasome

• Project/Area Number: 23657083
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: MURATA Shigeo 東京大学, 大学院・薬学系研究科, 教授 (20344070)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞内タンパク質分解 / プロテアソーム / ユビキチン / タンパク質分解 / ペプチド / 酵素

Research Abstract

The proteasome is the major proteolytic enzyme and irreversibly inactivate protein functions in cells. We assumed that short peptides generated by proteasomal degradation of cellular proteins have some physiological functions. To examine this hypothesis, we purified cytosolic and nuclear short peptides with C18-column. By using mass spectrometry, we succeeded in identifying sequences of these peptides. We also started to generate cell lines and mice in which peptide cleavage bythe proteasome is altered.

Research Products

• [Journal Article] Mouse zygote-specific proteasome assembly chaperone important for maternal-to-zygotic transition (2013)
  • Author(s): Shin SW
  • Journal Title: Biolology Open Volume: 2 Issue: 2 Pages: 170-182
  • DOI: 10.1242/bio.20123020
  • Peer Reviewed
• [Journal Article] Decreased proteasomal activity causes age-related phenotypes and promotes the development of metabolic abnormalities (2012)
  • Author(s): Tomaru U, Takahashi S, Ishizu A, Miyatake Y, Gohda A, Suzuki S, Ono A, Ohara J, Baba T, Murata S, Tanaka K, Kasahara M
  • Journal Title: Am.J.Pathol. Volume: 180 Issue: 3 Pages: 963-972
  • DOI: 10.1016/j.ajpath.2011.11.012
  • Peer Reviewed
• [Journal Article] Antiangiogenic tumor therapy by DNA vaccine inducing aquaporin-1-specific CTL based on ubiquitin-proteasome system in mice. (2012)
  • Author(s): Chou B, Hiromatsu K, Okano S, Ishii K, Duan X, Sakai T, Murata S, Tanaka K, Himeno K.
  • Journal Title: J Immunol Volume: 189 Issue: 4 Pages: 1618-26
  • DOI: 10.4049/jimmunol.1101971
  • Peer Reviewed
• [Journal Article] A mutation in the immunoproteasome subunit PSMB8 causes autoinflammation and lipodystrophy in humans (2011)
  • Author(s): Kitamura A, Maekawa Y, Uehara H, Izumi K, Kawachi I, Nishizawa M, Toyoshima Y, Takahashi H, Standley DM, Tanaka K, Hamazaki J, Murata S, Obara K, Toyoshima I, Yasutomo K.
  • Journal Title: J Clin Invest Volume: 121 Issue: 10 Pages: 4150-4160
  • DOI: 10.1172/jci58414
  • Peer Reviewed
• [Journal Article] Assembly defect due to a PSMB8 mutation reduces proteasome activity and causes the autoinflammatory disorder, Nakajo-Nishimura syndrome (2011)
  • Author(s): Arima, K., Kinoshita, A., Mishima, H., Kanazawa, N., Kaneko, T., Mizushima, T., Ichinose, K., Nakamura, H., Tsujino, A., Kawakami, A., Matsunaka, M., Kasagi, S., Kawano, S., Kumagai, S., Ohmura, K., Mimori, T., Hirano, M., Ueno, S., Tanaka, K., Tanaka, M., Toyoshima, I., Sugino, H., Yamakawa, A., Tanaka, K., Niikawa, N., Furukawa, F., Murata, S., Eguchi, K., Ida, H., Yoshiura, K.
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 108 Issue: 36 Pages: 14914-14919
  • DOI: 10.1073/pnas.1106015108
  • Peer Reviewed
• [Presentation] Enzymatic purification and mass spectrometric identification of polyubiquitinated proteins (2012)
  • Author(s): Naoyuki Mita, Jun Hamazaki, Shigeo Murata
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Investigation of the biological meaning of ss subunit diversity (2012)
  • Author(s): Akihiko Fukuda, Jun Hamazaki, Shigeo Murata
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Investigation of the Biological Meaning of β Subunit Diversity (2012)
  • Author(s): Akihiko Fukuda
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Enzymatic Purification and Mass Spectrometric Identification of Polyubiquitinated Proteins (2012)
  • Author(s): Naoyuki Mita
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] プロテアソームとミトコンドリアの遺伝学的相互作用 (2011)
  • Author(s): 白水亮平、八代田英樹、村田茂穂
  • Organizer: 第84回日本生化学会大会。
  • Place of Presentation: 京都
  • Year and Date: 2011-09-17
• [Presentation] プロテアソームとミトコンドリアの遺伝学的相互作用 (2011)
  • Author(s): 白水亮平、八代田英樹、村田茂穂
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Book] タンパク質分解系による生体制御 (2011)
  • Author(s): 反町洋之編
  • Total Pages: 242
  • Publisher: (羊土社)村田茂穂
• [Book] タンパク質分解系による生体制御 (2011)
  • Author(s): 村田茂穂、反町洋之 編集
  • Total Pages: 242
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~tanpaku/
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~tanpaku/