| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Research Abstract |
Chromatin, the nucleoprotein structure into which the eukaryotic genome is organized, enables essential biological processes such as regulation of transcription. A variety of remodeling events enable the architecture of chromatin to transition between a condensed and a decondensed state, each state being coupled to specific cellular functions. The covalent modifications occur on the N-terminal tails of the core histones H2A, H2B, H3 and H4. Several histone modifications contribute to chromatin remodeling and thereby to the control of a large array of nuclear processes. In addition, the disposition of the tails renders these domains accessible to modifications that could reversibly modulate chromatin structure. Although the death domain-associated protein DAXX has traditionally been associated with the regulation of cell death pathways, recent studies have demonstrated that DAXX can function as a histone chaperone.
Circadian clocks are intrinsic, time-tracking systems that endow organisms with a survival advantage. In mammals, the transcription factors, BMAL1, is an essential circadian regulator. I have identified BMAL1 as a novel DAXX-interacting protein. This study provides several lines of evidence indicating that DAXX-BMAL1 interaction may have important roles in dynamic changes in chromatin transitions.
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