Development of a novel light-regulated gene expression system
| Project/Area Number |
23657089
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MASUI Ryoji 大阪大学, 大学院・理学研究科, 准教授 (40252580)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 遺伝子の情報発現と複製 / 発現制御 / 蛋白質 / 遺伝子 / 細菌 |
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| Research Abstract |
A transcriptional regulator LitR of Thermus thermophilus HB8 acts as an repressor which binds to upstream region of carotenoid biosynthesis genes and releases repression depending blue light. This regulatory system was used for constructing a light-dependent gene expression system. A DNA fragment containing the litR gene and LitR-binding region was inserted into an expression vector containing a reporter gene in E. coli and T. thermophilus. The results of gene expression response to blue light irradiation suggested the feasibility of the light-dependent gene expression system using LitR.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Close proximity of phosphorylation sites to ligand in the phosphoproteome of the extreme thermophile2012
Author(s)
Takahata, Y., Inoue, M., Kim, K., Iio, Y., Miyamoto, M., Masui, R., Ishihama, Y., Kuramitsu, S
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Journal Title
Thermus thermophilus HB8. Proteomics
Volume: 12(9)
Pages: 1414-1430
Related Report
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