Development of a novel light-regulated gene expression system

• Project/Area Number: 23657089
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: MASUI Ryoji 大阪大学, 大学院・理学研究科, 准教授 (40252580)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 遺伝子の情報発現と複製 / 発現制御 / 蛋白質 / 遺伝子 / 細菌

Research Abstract

A transcriptional regulator LitR of Thermus thermophilus HB8 acts as an repressor which binds to upstream region of carotenoid biosynthesis genes and releases repression depending blue light. This regulatory system was used for constructing a light-dependent gene expression system. A DNA fragment containing the litR gene and LitR-binding region was inserted into an expression vector containing a reporter gene in E. coli and T. thermophilus. The results of gene expression response to blue light irradiation suggested the feasibility of the light-dependent gene expression system using LitR.

Research Products

• [Journal Article] Whole-cell proteome reference maps of an extreme thermophile, Thermus thermophilus HB8 (2012)
  • Author(s): Kwang Kim
  • Journal Title: Proteomics Volume: 12 Issue: 19-20 Pages: 3063-3068
  • DOI: 10.1002/pmic.201100375
  • Peer Reviewed
• [Journal Article] Close proximity of phosphorylation sites to ligand in the phosphoproteome of the extreme thermophile (2012)
  • Author(s): Takahata, Y., Inoue, M., Kim, K., Iio, Y., Miyamoto, M., Masui, R., Ishihama, Y., Kuramitsu, S
  • Journal Title: Thermus thermophilus HB8. Proteomics Volume: 12(9) Pages: 1414-1430
• [Journal Article] Close proximity of phosphorylation sites to ligand in the phosphoproteome of the extreme thermophile Thermus thermophilus HB8 (2012)
  • Author(s): Yoshio Takahata
  • Journal Title: Proteomics Volume: 12 Issue: 9 Pages: 1414-1430
  • DOI: 10.1002/pmic.201100573
  • Peer Reviewed
• [Presentation] モデル生物 Thermus thermophilus HB8 の蛋白質の翻訳後修飾 (2013)
  • Author(s): 増井良治、上利佳弘、新海暁男、飯尾洋太、岡西広樹、KwangKim、大山礼雅、西田優也、福井健二、倉光成紀
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜バイオ大学
  • Year and Date: 2013-03-10
• [Presentation] 高度好熱菌丸ごと一匹解析:リン酸化による翻訳後修飾 (2012)
  • Author(s): 飯尾洋太、高畑良雄、井上真男、金 光、福井健二、上利佳弘、新海暁男、増井良治、倉光成紀
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-15
• [Presentation] Whole cell analysis of Thermus thermophilus HB8: The regulation of mRNA decay by Rnases (2012)
  • Author(s): Hiromasa Ohyama, Yoshihiro Agari, Kenji Fukui, Noriko Nakagawa, Akeo Shinkai, Ryoji Masui, Seiki Kuramitsu
  • Organizer: 85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-15
• [Presentation] 短鎖 DNA/RNA 特異的エキソヌクレアーゼの基質認識機構の解明 (2012)
  • Author(s): 植村有里, 若松泰介, 中川紀子, 増井良治, 倉光成紀
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-15
• [Presentation] 極限微生物を利用した基本的生命現象の発見 (2012)
  • Author(s): 増井良治、上利佳弘、新海暁男、飯尾洋太、岡西広樹、金 光、大山礼雅、福井健二、倉光成紀
  • Organizer: 第13回極限環境生物学会年会
  • Place of Presentation: 日本大学文理学
  • Year and Date: 2012-12-02
• [Presentation] 高度好熱菌のリン酸化プロテオーム解析:立体構造に基づくリン酸化部位のマッピング (2012)
  • Author(s): 高畑良雄、井 上真男、飯 尾洋太、妻鹿良亮、高橋裕佳、友池史明、中川紀子、金 光、宮本昌明、石濱 泰、増井良治、倉光成紀
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-06-22
• [Presentation] 高度好熱菌由来 Ser/Thr protein kinase TTHA0138 の機能解析 (2012)
  • Author(s): 飯尾洋太、高畑良雄、井上真男、金 光、福井健二、上利佳弘、新海暁男、増井良治、倉光成紀
  • Organizer: 第59回日本生化学会近畿支部会例会
  • Place of Presentation: 京都大学宇治キャンパス おうばくプラザ
  • Year and Date: 2012-05-19
• [Presentation] Transcriptome analysis of RNase gene disruptants in Thermus thermophilus HB8 (2011)
  • Author(s): Hiromasa Ohyama,Yoshihiro Agari,Kenji Fukui,Noriko Nakagawa,Akeo Shinkai,Seiki Kuramitsu,Ryoji Masui
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] 原核生物における Ser/Thr/Tyr リン酸化タンパク質の網羅的解析 (2011)
  • Author(s): 高畑良雄,井上真男,金 光,石濱 泰,増井良治,倉光成紀
  • Organizer: 第58回日本生化学会近畿支部会例会
  • Place of Presentation: 関西医科大学滝井キャンパス
  • Year and Date: 2011-05-21