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Development of oriented reconstitution methods for membrane proteins

Research Project

Project/Area Number 23657091
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionOkayama University

Principal Investigator

OMOTE Hiroshi  岡山大学, 大学院・医歯薬学総合研究科, 准教授 (10273707)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords生体膜 / トランスポーター / タンパク質 / 生化学 / 神経 / 輸送 / 再構成 / リポソーム / バイオテクノロジー / ナノバイオ / 蛋白質
Research Abstract

Transporters contribute to various biological activity including uptake of nutrients, exclusion of waste products and drugs. It also contribute to signal transmission through their transport activity across the membrane. Measurement of transport activity is an essential part of functional study of transporters and development of novel drugs. In this study, we investigated the transport assay with reconstituted liposomes.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 5 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] 塩素イオンにより制御されるトランスポーター群の発見と意義2013

    • Author(s)
      表 弘志、森山芳則
    • Journal Title

      医学の歩み

      Volume: 245 Pages: 69-74

    • Related Report
      2012 Final Research Report
  • [Journal Article] Vesicular neurotransmitter transporters: an approach for studying transporters with purified proteins.2013

    • Author(s)
      Omote H
    • Journal Title

      Physiology

      Volume: 28 Issue: 1 Pages: 39-50

    • DOI

      10.1152/physiol.00033.2012

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Na+/phosphate co-transporter homologue (SLC17A4 protein) is an intestinal organic anion exporter.2012

    • Author(s)
      Togawa N
    • Journal Title

      Am J Physiol Cell Physiol

      Volume: 302 Issue: 11 Pages: C1652-C1660

    • DOI

      10.1152/ajpcell.00015.2012

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Divalent cation transport by vesicular nucleotide transporter2011

    • Author(s)
      Miyaji, T., Sawada, K., Omote, H.Moriyama Y
    • Journal Title

      J. Biol. Chem

      Volume: 286 Issue: 50 Pages: 42881-42887

    • DOI

      10.1074/jbc.m111.277269

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of vesicular excitatory amino acid transport by human sialin2011

    • Author(s)
      Miyaji T, Omote H, Moriyama Y
    • Journal Title

      J Neurochem

      Volume: 119 Issue: 1 Pages: 1-5

    • DOI

      10.1111/j.1471-4159.2011.07388.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vesicular neurotransmitter transporter: bioenergetics and regulation of glutamate transport.2011

    • Author(s)
      Omote, H. Miyaji, T, Juge, N. and Moriyama, Y.
    • Journal Title

      Biochemistry

      Volume: 50 Pages: 5558-65

    • Related Report
      2012 Final Research Report
  • [Journal Article] Characterization of he human MATE2 proton-coupled polyspecific organic cation exporter2011

    • Author(s)
      Komatsu, T., Hiasa, M., Miyaji, T.,Kanamoto, T., Matsumoto, T., Otsuka, M., Moriyama, Y. and Omote, H
    • Journal Title

      In.t J. Biochtem. Cell Biol.

      Volume: 43 Issue: 6 Pages: 913-918

    • DOI

      10.1016/j.biocel.2011.03.005

    • Related Report
      2012 Final Research Report
  • [Journal Article] Vesicular neurotransmitter transporter: bioenergetics and regulation of glutamate transport2011

    • Author(s)
      Omote, H.Miyaji, T., Juge, N. and Moriyama, Y
    • Journal Title

      Biochemistry

      Volume: 50 Issue: 25 Pages: 5558-5565

    • DOI

      10.1021/bi200567k

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] NPTホモログは新トランスポーターである2012

    • Author(s)
      表 弘志
    • Organizer
      日本生体エネルギー研究会 第38回討論会
    • Place of Presentation
      岡山
    • Related Report
      2012 Final Research Report
  • [Presentation] NPTホモログは新規尿酸排出トランスポーターである2012

    • Author(s)
      表 弘志
    • Organizer
      生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] NPTホモログは新規尿酸排出トランスポーターである2012

    • Author(s)
      表弘志
    • Organizer
      日本生体エネルギー研究会
    • Place of Presentation
      岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] NPTホモログは新規尿酸トランスポーターである2012

    • Author(s)
      表弘志
    • Organizer
      生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] 小胞型グルタミン酸トランスポーターの活性制御2011

    • Author(s)
      表 弘志
    • Organizer
      日本生体エネルギー研究会 第36回討論会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 小胞型神経伝達物質トランスポーターの機能制御2011

    • Author(s)
      表 弘志
    • Organizer
      生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      岡山
    • Related Report
      2012 Final Research Report
  • [Presentation] 小胞型神経伝達物質トランスポーターの機能制御2011

    • Author(s)
      表弘志
    • Organizer
      生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      岡山大学創立五十周年記念館
    • Related Report
      2011 Research-status Report
  • [Presentation] 小胞型神経伝達物質トランスポーターの活性制御2011

    • Author(s)
      表弘志
    • Organizer
      生体エネルギー研究会
    • Place of Presentation
      京都産業大学
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.pharm.okayama-u.ac.jp/lab/seika/home/index.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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