Novel functions of ASF/SF2 in somatic hypermutation on the immunoglobulin gene.
Project/Area Number |
23657092
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
|
Research Institution | Okayama University |
Principal Investigator |
KANAYAMA Naoki 岡山大学, 大学院・自然科学研究科, 准教授 (70304334)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 抗体 / 親和性成熟 / 体細胞高頻度突然変異 / スプライシング因子 / ASF/SF2 / SRSF1 / 国際情報交流 / 米国 |
Research Abstract |
The affinity of antibodies for antigens is improved by somatic hypermutation (SHM) occurred on the immunoglobulin (Ig) gene. In this study, using the hypermutating chicken B cell line DT40, we found that an isoform of the alternative-splicing factor ASF/SF2, ASF3 is essential for SHM on the Ig gene. The results also suggest that ASF3 might have a role in the activation and targeting of the mutation machinery through the regulation of post-transcriptional processing on the Ig gene.
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Report
(3 results)
Research Products
(24 results)