Analyses of intracellular signals using a novel activation method for small GTPases

• Project/Area Number: 23657095
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MAEHAMA Tomohiko 国立感染症研究所, 細胞化学部, 室長 (40322755)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞情報伝達機構 / G蛋白質 / シグナル伝達 / Gタンパク質

Research Abstract

Small GTPases play pivotal roles in diverse intracellular signal transduction pathways. In this study, we applied RIRAG method to several small GTPases and found novel signaling pathway that ERK activation was induced by CDC42. The RIRAG method, in the case of CDC42-ERK pathway, was able to dissect the signaling by minute-order. The method potentially becomes a novel tool for the analysis of small GTPase-mediated signaling pathways, although RIRAG method has difficulties in controlling some small GTPases.

Research Products

• [Journal Article] 核小体を起点としp53を制御する新規分子PICT1による細胞増殖制御機構 (2013)
  • Author(s): 西尾美希、河原康一、佐々木雅人、前濱朝彦、佐々木雄彦、三森功士、森正樹、鈴木聡
  • Journal Title: 実験医学増刊 Volume: 31 Pages: 127-134
• [Journal Article] 核小体を起点としp53を制御する新規分子PICT1による細胞増殖制御機構 (2013)
  • Author(s): 西尾美希、ら
  • Journal Title: 実験医学（増刊） Volume: 31 Pages: 127-134
• [Journal Article] A new PICTure of nucleolar stress (2012)
  • Author(s): Suzuki A, Kogo R, Kawahara K, Sasaki M, Nishio M, Maehama T, Sasaki T, Mimori K, Mori M.
  • Journal Title: Cancer Sci Volume: 103 Issue: 4 Pages: 632-7
  • DOI: 10.1111/j.1349-7006.2012.02219.x
  • Peer Reviewed
• [Journal Article] Regulation of the MDM2-P53 pathway and tumor growth by PICT1 via nucleolar RPL11 (2011)
  • Author(s): Sasaki M, Kawahara K, Nishio M, Mimori K, Kogo R, Hamada K, Itoh B, Wang J, Komatsu Y, Yang YR, Hikasa H, Horie Y, Yamashita T, Kamijo T, Zhang Y, Zhu Y, Prives C, Nakano T, Mak TW, Sasaki T, Maehama T, Mori M, Suzuki A
  • Journal Title: Nat Med Volume: 17 Issue: 8 Pages: 944-951
  • DOI: 10.1038/nm.2392
  • Peer Reviewed
• [Presentation] Nucleolar stress induces ubiquitin-independent proteasomal degradation of PICT1 (2013)
  • Author(s): Maehama, T., Kawahara, K., Nishio, M., Suzuki, A
  • Organizer: 10th International Conference on Protein Phosphatase
  • Place of Presentation: Tokyo, Japan
• [Presentation] Nucleolar stress induces ubiquitin-independent proteasomal degradation of PICT1 (2013)
  • Author(s): Tomohiko Maehama, et al
  • Organizer: 10th International Conference on Protein Phosphatase
  • Place of Presentation: Tokyo
• [Presentation] mTORC1制御に関与するGタンパク質群の解析 (2012)
  • Author(s): 前濱朝彦
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] mTORC1制御に関与するGタンパク質群の解析 (2012)
  • Author(s): 前濱朝彦
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Remarks]
  • URL: http://www.nih.go.jp/niid/ja/from-biochem/912-4th.html