Development of microfluidic cell based on light guide for the single molecule fluorescence measurements
| Project/Area Number |
23657097
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KAMAGATA Kiyoto 東北大学, 多元物質科学研究所, 教授 (90432492)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 一分子観察 / 蛍光 / ライトガイド / フローセル / 微細加工 / ライトガイド型フローセル / 一分子蛍光観察 / タンパク質 / 長時間観察 |
|---|
| Research Abstract |
We developed several flow cells for the single molecule fluorescence measurements. The first can maintain stable sheath flow. The second can mix two solutions for the kinetic measurements.
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Report
(3 results)
Research Products
(53 results)
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[Journal Article] Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence2013
Author(s)
Oikawa, H., Suzuki, Y., Saito, M., Kamagata, K., Arai, M., & Takahashi, S.
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Journal Title
Scientific Reports
Volume: 3
Issue: 1
Pages: 2151-2151
DOI
Related Report
Peer Reviewed
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[Journal Article] Hydration-State Change of horse Heart Cytochrome c Corresponding to Trifluoroacetic-Acid-Induced unfolding2013
Author(s)
Miyashita, Y., Wazawa, T., Mogami, G., Takahashi, S., Sambongi, Y., Suzuki, M
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Journal Title
Biophysical Journal
Volume: 104
Pages: 163-172
Related Report
Peer Reviewed
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[Journal Article] Long-term observation of fluorescence ofgree single molecules to explore protein- folding energy landscapes2013
Author(s)
Kamagata, K., Kawaguchi, T., Iwahashi, Y., Baba, A., Fujimoto, K., Komatsuzaki, T., Sambongi, Y., Goto, Y., Takahashi, S
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Journal Title
Journal of American Chemical Society
Volume: 134
Pages: 11525-11532
Related Report
Peer Reviewed
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