Development of microfluidic cell based on light guide for the single molecule fluorescence measurements

• Project/Area Number: 23657097
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Satoshi 東北大学, 多元物質科学研究所, 教授 (30283641)
• Co-Investigator(Renkei-kenkyūsha): KAMAGATA Kiyoto 東北大学, 多元物質科学研究所, 教授 (90432492)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 一分子観察 / 蛍光 / ライトガイド / フローセル / 微細加工 / ライトガイド型フローセル / 一分子蛍光観察 / タンパク質 / 長時間観察

Research Abstract

We developed several flow cells for the single molecule fluorescence measurements. The first can maintain stable sheath flow. The second can mix two solutions for the kinetic measurements.

Research Products

• [Journal Article] Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence (2013)
  • Author(s): Oikawa, H., Suzuki, Y., Saito, M., Kamagata, K., Arai, M., & Takahashi, S.
  • Journal Title: Scientific Reports Volume: 3 Issue: 1 Pages: 2151-2151
  • DOI: 10.1038/srep02151
  • Peer Reviewed
• [Journal Article] A New Way to Degrade Heme -THE MYCOBACTERIUM TUBERCULOSIS ENZYME MhuD CATALYZES HEME DEGRADATION WITHOUT GENERATING CO (2013)
  • Author(s): Nambu, S., Matsui, T., Goulding, C. W., Takahashi, S., Ikeda-Saito, M.
  • Journal Title: Journal of BiologicalChemistry Volume: 288 Pages: 10101-10109
  • Peer Reviewed
• [Journal Article] Hydration-State Change of horse Heart Cytochrome c Corresponding to Trifluoroacetic-Acid-Induced unfolding (2013)
  • Author(s): Miyashita, Y., Wazawa, T., Mogami, G., Takahashi, S., Sambongi, Y., Suzuki, M
  • Journal Title: Biophysical Journal Volume: 104 Pages: 163-172
  • Peer Reviewed
• [Journal Article] Long-term observation of fluorescence ofgree single molecules to explore protein- folding energy landscapes (2013)
  • Author(s): Kamagata, K., Kawaguchi, T., Iwahashi, Y., Baba, A., Fujimoto, K., Komatsuzaki, T., Sambongi, Y., Goto, Y., Takahashi, S
  • Journal Title: Journal of American Chemical Society Volume: 134 Pages: 11525-11532
  • Peer Reviewed
• [Journal Article] A new way to degrade heme: The Mycobacterium tuberculosis enzyme MhuD catalyzes heme degradation without generating CO (2013)
  • Author(s): S. Nambu, T. Matsui, C. W. Goulding, S. Takahashi, M. Ikeda-Saito
  • Journal Title: J. Biol. Chem. Volume: 印刷中 Issue: 14 Pages: 10101-10109
  • DOI: 10.1074/jbc.m112.448399
  • Peer Reviewed
• [Journal Article] Long-Term Observation of Fluorescence of Free SingleMolecules To Explore Protein-Folding Energy Landscapes (2012)
  • Author(s): Kamagata K, Kawaguchi T, Iwahashi Y, Baba A, Fujimoto K, Komatsuzaki T, Sambongi Y, Goto Y, Takahashi S
  • Journal Title: J. Am. Chem. Soc. Volume: 134 Issue: 28 Pages: 11525-11532
  • DOI: 10.1021/ja3020555
  • Peer Reviewed
• [Journal Article] Staring at a Protein: Ensemble and Single Molecule Investigations on Protein Folding Dynamics (2012)
  • Author(s): Takahashi, S., Kamagata, K.
  • Journal Title: Single-Molecule Biophysics : Experiment and Theory in Adv. Chem. Phys Volume: 146 Pages: 3-22
  • Peer Reviewed
• [Journal Article] 新しいタンパク質化学の可能性 ～新時代を切り拓くマルチタレントDavid Baker～ (2011)
  • Author(s): 鎌形清人、高橋聡
  • Journal Title: 化学 Volume: 66 Pages: 68-69
• [Journal Article] 一分子蛍光観察法を用いたタンパク質フォールディングの解明 (2011)
  • Author(s): 小井川浩之、鎌形清人、高橋聡
  • Journal Title: 高分子 Volume: 60 Pages: 801-803
  • NAID: 10029795631
• [Presentation] 一分子蛍光計測によるタンパク質の折り畳みや機能ダイナミクス (2013)
  • Author(s): 鎌形清人
  • Organizer: 日本物理学会第68回年次大会
  • Place of Presentation: 広島市
• [Presentation] 高速一分子蛍光追跡による柔らかなタンパク質の運動追跡 (2013)
  • Author(s): 高橋聡
  • Organizer: 日本化学会第93回春季年会
  • Place of Presentation: 草津市
• [Presentation] Microsecond-Resolved tracking of the Unfolded State of BdpA by a Line Confocal Detection of Single Molecule Fluorescence (2013)
  • Author(s): Satoshi Takahashi
  • Organizer: International Symposium on Protein Folding and Its Biological Significance
  • Place of Presentation: 岡崎市
• [Presentation] Microsecond-Resolved tracking of the Unfolded State of BdpA by a Line Confocal Detection of Single Molecule Fluorescence (2013)
  • Author(s): Satoshi Takahashi
  • Organizer: International Symposium on Protein Folding and Its Biological Significance
  • Place of Presentation: 岡崎
  • Invited
• [Presentation] 高速一分子蛍光追跡による柔らかなタンパク質の運動追跡 (2013)
  • Author(s): 高橋聡
  • Organizer: 日本化学会第９３回春季年会
  • Place of Presentation: 草津
  • Invited
• [Presentation] Single-molecule fluorescence and diffusion measurements of a GroEL-binding peptide (2012)
  • Author(s): Suguru Watanabe, Shion Ando, Kiyoto Kamagata, Tatsuya Nojima, Hideki Taguchi, Takahiro Hohsaka, Satoshi Takahashi
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡市
• [Presentation] Microsecond-resolved tracking of the unfolded state of BdpA by a line confocal detection of single molecule fluorescence (2012)
  • Author(s): Hiroyuki Oikawa, Yuta Suzuki, Masataka Saito, Kiyoto Kamagata, Munehito Arai , Satsohi Takahashi
  • Organizer: Indo- Japan Workshop on'Recent Advances in Spectroscopy and Microscopy: Fundamentals and Applications to Materials and Biology
  • Place of Presentation: India, Hyderabad
• [Presentation] Microsecond-Resolved Tracking of The Unfold State of BdpA by a Line Confocal Detection of Single Molecule Fluorescence (2012)
  • Author(s): Satoshi Takahashi
  • Organizer: International Symposium on Protein Folding and Dynamics
  • Place of Presentation: India, Bangalore
• [Presentation] Single-molecule structuralanalysis of tumor suppressor p53 using scanning probe microscopes (2012)
  • Author(s): Seiya Takahashi, Yasuyuki Sainoo, Risa kashima, Takashi Tokino, Tadahiro Komeda, Satoshi Takahashi, Kiyoto Kamagata
  • Organizer: 第50回日本生物物理学会
  • Place of Presentation: 名古屋市
• [Presentation] Microsecond-resolved traces of protein folding by single-molecule FRET measurements (2012)
  • Author(s): Hiroyuki Oikawa, Yuta Suzuki, Kiyoto Kamagata, Munehito Arai, Satoshi Takahashi
  • Organizer: 第50回日本生物物理学会
  • Place of Presentation: 名古屋市
• [Presentation] Construction of a microfluidic system for fluorescence-based single-molecule sorting (2012)
  • Author(s): Sho Saitoh, Hiroyuki Oikawa, Kiyoto Kamagata, Satoshi Takahashi
  • Organizer: 第50回日本生物物理学会
  • Place of Presentation: 名古屋市
• [Presentation] 新規-分子蛍光観測法による-分子蛍光観察によるタンパク質のエネルギー地形解析 (2012)
  • Author(s): 高橋聡、小井川浩之、鎌形清人
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋市
• [Presentation] 新規-分子蛍光観測法による蛋白質の折り畳みの自由エネルギー地形の探索 (2012)
  • Author(s): 鎌形清人、川口敏史、馬場昭典、三本木至宏、小松崎民樹、高橋聡
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋市
• [Presentation] マイクロ秒分解-分子FRET測定による蛋白質構造変化の追跡 (2012)
  • Author(s): 小井川浩之、鎌形清人、飯島一生、芳坂貴弘、高橋聡
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋市
• [Presentation] 一分子蛍光観察によるタンパク質のエネルギー地形解析 (2012)
  • Author(s): 高橋聡,小井川浩之,鎌形清人
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋
• [Presentation] マイクロ秒分解一分子FRET測定による蛋白質構造変化の追跡 (2012)
  • Author(s): 小井川浩之, 鎌形清人, 飯島一生, 芳坂貴弘, 高橋聡
  • Organizer: 第12回日本蛋白質科学会年
  • Place of Presentation: 名古屋
• [Presentation] 新規一分子蛍光観測法による蛋白質の折り畳みの自由エネルギー地形の探索 (2012)
  • Author(s): 鎌形清人, 川口敏史, 馬場昭典, 三本木至宏, 小松崎民樹, 高橋聡
  • Organizer: 第12回日本蛋白質科学会年
  • Place of Presentation: 名古屋
• [Presentation] マイクロ秒分解一分子蛍光検出による変性状態タンパク質の高速構造揺らぎ (2012)
  • Author(s): 小井川浩之, 鎌形清人, 新井宗仁, 高橋聡
  • Organizer: 新学術領域「揺らぎと生体機能」「水和とATP」合同公開シンポジウム「ゆらぎと水 - 生命のエネルギーと機能の分子機構を探る」
  • Place of Presentation: 大阪
• [Presentation] 新規一分子FRET計測技術によるABCトランスポーターのATP加水分解機構の解析 (2012)
  • Author(s): 中野駿介, 七谷圭 , 魚住信之, 高橋聡, 鎌形清人
  • Organizer: 新学術領域「揺らぎと生体機能」「水和とATP」合同公開シンポジウム「ゆらぎと水 - 生命のエネルギーと機能の分子機構を探る」
  • Place of Presentation: 大阪
• [Presentation] Construction of a microfluidic system for fluorescence-based single-molecule sorting (2012)
  • Author(s): Sho Saitoh, Hiroyuki Oikawa, Kiyoto Kamagata, Satoshi Takahashi
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
• [Presentation] Microsecond-resolved traces of protein folding by single-molecule FRET measurements (2012)
  • Author(s): Hiroyuki Oikawa, Yuta Suzuki, Kiyoto Kamagata, Munehito Arai, Satoshi Takahashi
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
• [Presentation] Single-molecule structural analysis of tumor suppressor p53 using scanning probe microscopes (2012)
  • Author(s): Seiya Takahashi, Yasuyuki Sainoo, Risa kashima, Takashi Tokino, Tadahiro Komeda, Satoshi Takahashi, Kiyoto Kamagata
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
• [Presentation] Microsecond-Resolved Tracking of The Unfolded State of BdpA by a Line Confocal Detection of Single Molecule Fluorescence (2012)
  • Author(s): Satoshi Takahashi
  • Organizer: International Symposium on Protein Folding and Dynamics
  • Place of Presentation: India,Bangalore
  • Invited
• [Presentation] 高速一分子FRET追跡によるタンパク質のフォールディングダイナミクス (2012)
  • Author(s): 高橋聡
  • Organizer: 最近の光源開発・計測法の進歩と分光学への応用
  • Place of Presentation: 京都
  • Invited
• [Presentation] Microsecond-resolved tracking of the unfolded state of BdpA by a line confocal detection of single molecule fluorescence (2012)
  • Author(s): Hiroyuki Oikawa, Yuta Suzuki, Masataka Saito, Kiyoto Kamagata, Munehito Arai, Satoshi Takahashi
  • Organizer: Indo- Japan Workshop on 'Recent Advances in Spectroscopy and Microscopy: Fundamentals and Applications to Materials and Biology
  • Place of Presentation: India,Hyderabad
  • Invited
• [Presentation] Microsecond-resolved tracking of the unfolded state of BdpA by a line confocal detection of single molecule fluorescence (2012)
  • Author(s): Hiroyuki Oikawa,Yuta Suzuki, Masataka Saito, Kiyoto Kamagata, Munehito Arai, Satoshi Takahashi
  • Organizer: 新学術領域研究「揺らぎと生体機能」第６回公開シンポジウム
  • Place of Presentation: 京都
• [Presentation] Construction of a microfluidic system for fluorescence based single-molecule sorting (2012)
  • Author(s): Saitoh, HIroyuki Oikawa, Kiyoto Kamagata, Satsohi Takahashi
  • Organizer: 第12回東北大学多元物質科学研究所研究発表会
  • Place of Presentation: 仙台
• [Presentation] Single-molecule fluorescence and diffusion measurements of a GroEL-binding peptide (2012)
  • Author(s): Suguru Watanabe, Shion Ando, Kiyoto Kamagata, Tatsuya Nojima, Hideki Taguchi, Takahiro Hohsaka, Satoshi Takahashi
  • Organizer: 第85回日本生物化学会大会
  • Place of Presentation: 福岡
• [Presentation] GroEL結合ペプチドの一分子蛍光拡散測定 (2012)
  • Author(s): 渡邉優, 安藤嗣音, 鎌形清人, 野島達也, 田口英樹, 芳坂貴弘, 高橋聡
  • Organizer: 日本生物物理学会東北支部会2012
  • Place of Presentation: 仙台
• [Presentation] マイクロ秒分解一分子FRET測定によるタンパク質折り畳み運動の追跡 (2012)
  • Author(s): 小井川浩之
  • Organizer: 日本生物物理学会東北支部会2012
  • Place of Presentation: 仙台
• [Presentation] 人工タンパク質のデザインを目指した蛍光一分子ソーターの開発 (2012)
  • Author(s): 斉藤翔, 小井川浩之, 鎌形清人, 高橋聡
  • Organizer: 日本生物物理学会東北支部会2012
  • Place of Presentation: 仙台
• [Presentation] Exploring The Energy Landscape of Denatured Protein Using Time-series Detection of Fluorescence of Free Single Molecules (2012)
  • Author(s): Kiyoto Kamagata, Toshifumi Kawaguchi, Akinori Baba, Yoshihiro Sambongi, Tamiki Komatsuzaki, and Satoshi Takahashi
  • Organizer: International Symposium on Innovative Nanobiodevices ISIN 2012
  • Place of Presentation: Nagoya
• [Presentation] Folding dynamics of cytochrome c551 studied by long time observation of fluorescence time series from single molecules (2012)
  • Author(s): Toshifumi Kawaguchi, Kiyoto Kamagata, Hiroyuki Oikawa, Yoshihiro Sambongi, Satsohi Takahashi
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 横浜
• [Presentation] Imaging and tracking of single BLG molecules in solution (2011)
  • Author(s): Sion Ando, Hiroyuki Oikawa, Kiyoto Kamagata, Kazumasa Sakurai, Yuji Goto, Satoshi Takahashi
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] Microsecond-resolved time traces of protein conformational change by single-molecule fluorescence detection (2011)
  • Author(s): Hiroyuki Oikawa, Kiyoto Kamagata, Issei Iijima, Takahiro Hohsaka, Yoshihiro Sambongi, Satoshi Takahashi
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] Additives inhibiting the bleaching of fluorophores for the oong-time fluorescence observation of single proteins (2011)
  • Author(s): Hironao Itabashi, Eriko Mano, Hiroyuki Oikawa, Yasuyuki Araki, Kiyoto Kamagata, Takehiko Wada, Satoshi Takahashi
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] Energy landscape of denatured protein revealed by a new singe-molecule fluorescence method (2011)
  • Author(s): Kiyoto Kamagata, Toshifumi Kawaguchi, Akinori Baba, Yoshihiro Sambongi, Tamiki Komatsuzaki, Satoshi Takahashi
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] Submillisecond dynamics of protein folding and ligand binding detected by the line conforcal microscopy (2011)
  • Author(s): Hiroyuki Oikawa, Kiyoto Kamagata, Issei Iijima, Takahiro Hosaka, Yoshihiro Sambongi, Satoshi Takahashi
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] イクロ秒分解一分子蛍光検出による蛋白質構造変化の追跡 (2011)
  • Author(s): 小井川浩之、鎌形清人、飯島一生、芳坂貴弘、高橋聡
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波
• [Presentation] 新規一分子蛍光観測法による蛋白質の自由エネルギー地形の解析 (2011)
  • Author(s): 鎌形清人、川口敏史、馬場昭典、三本木至宏、小松崎民樹、高橋聡
  • Organizer: 第１１回日本蛋白質科学会年会
  • Place of Presentation: 大阪
• [Presentation] マイクロ秒分解一分子蛍光検出による蛋白質構造変化の追跡 (2011)
  • Author(s): 小井川浩之、鎌形清人、飯島一生、芳坂貴弘、高橋聡
  • Organizer: 第１１回日本蛋白質科学会年会
  • Place of Presentation: 大阪
• [Presentation] Single molecule and ensemble investigations of protein folding dynamics in the time domain from 30 μs to seconds (2011)
  • Author(s): Satoshi Takahashi
  • Organizer: The 16th Biophysics Conference(招待講演)
  • Place of Presentation: 台湾
• [Book] Protein Folding and Misfolding Shining Light by Infrared Spectroscopy, Watching Dynamical Events in Protein Folding in the Time Domain from Submilliseconds to Second: Continuous-Flow Rapid-Mixing Infrared Spectroscopy (2011)
  • Author(s): Satoshi Takahashi, Tetsunari Kimura
  • Publisher: Springer
• [Remarks]
  • URL: http://www.tagen.tohoku.ac.jp/modules/laboratory/index.php?laboid=34