Development of biocompatible fluorescent silicon nanocrystals for fluorescence cell imaging
Project/Area Number |
23657101
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Biophysics
|
Research Institution | Kyoto University |
Principal Investigator |
KUSUMI Akihiro 京都大学, 物質-細胞統合システム拠点, 教授 (50169992)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | 応用光学・量子光工学 / 複合材料・物性 / マイクロ・ナノデバイス / 量子ドット / シグナル伝達 |
Research Abstract |
Fluorescence microscopy is used extensively in biomedical research, but it is often plagued by three major problems with the presently available fluorescent probes: photobleaching, blinking, and large size. We have developed biocompatible, fluorescent silicon nanocrystals (SiNCs), with special attention to single-molecule imaging. Methods for producing SiNCs by simple chemical etching, for hydrophilically coating them, and for conjugating precisely 1 SiNC to 1 protein molecule were developed. Single SiNCs neither blinked nor photobleached during a 300-min overall period observed at video rate. Single receptor molecules in the plasma membrane of living cells were imaged for ≥10 times longer than with other probes, making it possible for the first time to observe the internalization process of receptor molecules at the single-molecule level. Micron-scale spatial variations of molecular diffusivity, i.e., a higher level of domain mosaicism in the plasma membrane, were revealed.
|
Report
(4 results)
Research Products
(26 results)
-
[Journal Article] Biexciton state causes photoluminescence fluctuations in CdSe/ZnS core/shell quantum dots at high photoexcitation densities2013
Author(s)
N. Yoshikawa, H. Hirori, H. Watanabe, T. Aoki, T. Ihara, R. Kusuda, C. Wolpert, T. K. Fujiwara, A. Kusumi, Y. Kanemitsu, and K. Tanaka
-
Journal Title
Physical Review B
Volume: Vol.88
Issue: 15
DOI
Related Report
Peer Reviewed
-
[Journal Article] Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging2013
Author(s)
Nishimura H, Ritchie K, Kasai RS, Morone N, Sugimura H, Tanaka K, Sase I, Yoshimura A, Nakano Y, Fujiwara TK, and Kusumi A
-
Journal Title
J Cell Biol.
Volume: 202
Issue: 6
Pages: 967-83
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone2012
Author(s)
A. C. E. Shibata, T. K. Fujiwara, L. Chen, K. G. N. Suzuki, Y. Ishikaia, Y. L. Nemoto, Y. Miwa, Z. Kalay, R. Chadda, K. Naruse, and A. Kusumi
-
Journal Title
Cytoskeleton
Volume: 69
Issue: 6
Pages: 380-392
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-