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Development of biocompatible fluorescent silicon nanocrystals for fluorescence cell imaging

Research Project

Project/Area Number 23657101
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionKyoto University

Principal Investigator

KUSUMI Akihiro  京都大学, 物質-細胞統合システム拠点, 教授 (50169992)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords応用光学・量子光工学 / 複合材料・物性 / マイクロ・ナノデバイス / 量子ドット / シグナル伝達
Research Abstract

Fluorescence microscopy is used extensively in biomedical research, but it is often plagued by three major problems with the presently available fluorescent probes: photobleaching, blinking, and large size. We have developed biocompatible, fluorescent silicon nanocrystals (SiNCs), with special attention to single-molecule imaging. Methods for producing SiNCs by simple chemical etching, for hydrophilically coating them, and for conjugating precisely 1 SiNC to 1 protein molecule were developed. Single SiNCs neither blinked nor photobleached during a 300-min overall period observed at video rate. Single receptor molecules in the plasma membrane of living cells were imaged for ≥10 times longer than with other probes, making it possible for the first time to observe the internalization process of receptor molecules at the single-molecule level. Micron-scale spatial variations of molecular diffusivity, i.e., a higher level of domain mosaicism in the plasma membrane, were revealed.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (26 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (17 results) (of which Invited: 7 results) Remarks (1 results)

  • [Journal Article] Biexciton state causes photoluminescence fluctuations in CdSe/ZnS core/shell quantum dots at high photoexcitation densities2013

    • Author(s)
      N. Yoshikawa, H. Hirori, H. Watanabe, T. Aoki, T. Ihara, R. Kusuda, C. Wolpert, T. K. Fujiwara, A. Kusumi, Y. Kanemitsu, and K. Tanaka
    • Journal Title

      Physical Review B

      Volume: Vol.88 Issue: 15

    • DOI

      10.1103/physrevb.88.155440

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging2013

    • Author(s)
      Nishimura H, Ritchie K, Kasai RS, Morone N, Sugimura H, Tanaka K, Sase I, Yoshimura A, Nakano Y, Fujiwara TK, and Kusumi A
    • Journal Title

      J Cell Biol.

      Volume: 202 Issue: 6 Pages: 967-83

    • DOI

      10.1083/jcb.201301053

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Racl recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis2013

    • Author(s)
      A. C. E. Shibata, L. H. Chen, R. Nagai, F. Ishidate, R. Chadda, Y. Miwa, K. Naruse, Y. M. Shirai, T. K. Fujiwara, and A. Kusumi
    • Journal Title

      Cytoskeleton

      Volume: 70 Issue: 3 Pages: 161-177

    • DOI

      10.1002/cm.21097

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dynamic organizing principles of the plasma membrane that regulate signal transduction : commemorating the fortieth anniversary of Singer and Nicolson's fluid-mosaic model.2012

    • Author(s)
      Kusumi A, Fujiwara TK, Chadda R, Xie M, Tsunoyama TA, Kalay Z, Kasai RS, Suzuki KG.
    • Journal Title

      Annual review of cell and developmental biology

      Volume: 28 Issue: 1 Pages: 215-50

    • DOI

      10.1146/annurev-cellbio-100809-151736

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Membrane mechanisms for signal transduction : The coupling of the meso-scale raft domains to membrane-skeleton-induced compartments and dynamic protein complexes2012

    • Author(s)
      A.Kusumi, T.K.Fujiwara, N.Morone, K.J.Yoshida, R.Chadda, M.Xie, R.S.Kasai, K.G.N.Suzuki
    • Journal Title

      Semin.Cell.Dev.Biol.

      Volume: 23(2) Issue: 2 Pages: 126-144

    • DOI

      10.1016/j.semcdb.2012.01.018

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone2012

    • Author(s)
      A. C. E. Shibata, T. K. Fujiwara, L. Chen, K. G. N. Suzuki, Y. Ishikaia, Y. L. Nemoto, Y. Miwa, Z. Kalay, R. Chadda, K. Naruse, and A. Kusumi
    • Journal Title

      Cytoskeleton

      Volume: 69 Issue: 6 Pages: 380-392

    • DOI

      10.1002/cm.21032

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hierarchical meso-scale somain organization of the plasma membrane2011

    • Author(s)
      A.Kusumi, K.G.N.Suzuki, R.S.Kasai, K.Ritchie, T.K.Fujiwara
    • Journal Title

      Trends Biochem.Sci.

      Volume: 36 Issue: 11 Pages: 604-615

    • DOI

      10.1016/j.tibs.2011.08.001

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cytoskeleton-induced meso-scale domains2011

    • Author(s)
      Z. Kalay, T. K. Fujiwara, and A. Kusumi
    • Journal Title

      Cellular Domains

      Volume: Chapter 1 Pages: 3-22

    • DOI

      10.1002/9781118015759.ch1

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Dynamic organizing principles of the plasma membrane that regulate signal transduction : single-molecule tracking studies2012

    • Author(s)
      楠見明弘
    • Organizer
      Departmental Seminar
    • Place of Presentation
      Dept. of Physiology, University of Otago, University of Otago(ニュージーランド)
    • Year and Date
      2012-11-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Organizing principles of the plasma membrane : Three-tiered hierarchical meso-scale domain architecture revealed by single-molecule tracking2012

    • Author(s)
      楠見明弘
    • Organizer
      Joint Summer Research School in Nanomedicine
    • Place of Presentation
      慶應義塾大学(東京)
    • Year and Date
      2012-08-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Signal transduction of GPI-anchored receptors based on dimerization and raft-lipid interaction2012

    • Author(s)
      楠見明弘
    • Organizer
      The 105th International Titisee Conferences
    • Place of Presentation
      Treschers Schwarzwaldhotel am See(ドイツ)
    • Year and Date
      2012-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Signal transduction of GPI-anchored receptors based on dimerization and raft-lipid interaction2012

    • Author(s)
      A. Kusumi
    • Organizer
      The International Titisee Conferences(招待講演)
    • Place of Presentation
      ティティーゼ(ドイツ)
    • Related Report
      2011 Research-status Report
  • [Presentation] Lipid-Stabilized Dynamic Homo-Dimers of GPI-Anchored Receptors Convert into Oligomeric Signaling Clusters2011

    • Author(s)
      楠見明弘
    • Organizer
      The 51st Annual Meeting of the American Society for Cell Biology
    • Place of Presentation
      Colorado Convention Center(アメリカ)
    • Year and Date
      2011-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Organizing principle of the plasma membrane : three-tiered meso-scale domain architecture revealed by single-molecule tracking2011

    • Author(s)
      楠見明弘
    • Organizer
      The 8th European Biophysics Congress
    • Place of Presentation
      Eotvos Lorand University(ハンガリー)
    • Year and Date
      2011-08-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Raft-facilitated protein interactions for signal transduction as revealed by single-molecule imaging2011

    • Author(s)
      楠見明弘
    • Organizer
      The 30th Naito Conference "Membrane Dynamics and Lipid Biology [II] : Domains, Droplets and Diseases"
    • Place of Presentation
      シャトレーゼガトーキングダムサッポロ(北海道)
    • Year and Date
      2011-06-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Raft-facilitated protein interactions for signal transduction as revealed by single-molecule imaging2011

    • Author(s)
      A. Kusumi
    • Organizer
      The 30th Naito Conference. "Membrane Dynamics and Lipid Biology [II]: Domains, Droplets and Diseases."(招待講演)
    • Place of Presentation
      シャトレーゼ ガトーキングダム(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] Organizing principle of the plasma membrane: three-tiered meso-scale domain architecture revealed by single-molecule tracking2011

    • Author(s)
      A. Kusumi
    • Organizer
      The 8th European Biophysics Congress(招待講演)
    • Place of Presentation
      ブダペスト(ハンガリー)
    • Related Report
      2011 Research-status Report
  • [Presentation] Lipid-Stabilized Dynamic Homo-Dimers of GPI-Anchored Receptors Convert into Oligomeric Signaling Clusters2011

    • Author(s)
      A. Kusumi
    • Organizer
      The American Society for Cell Biology 2011 Annual Meeting(招待講演)
    • Place of Presentation
      デンバー(アメリカ)
    • Related Report
      2011 Research-status Report
  • [Presentation] 細胞膜上の特別なドメインの形成機構と機能

    • Author(s)
      楠見明弘
    • Organizer
      第27回動物細胞工学シンポジウム 「細胞表面の理解と応用」
    • Place of Presentation
      東京工業大学キャンパス・イノベーションセンター(東京)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 1分子毎に見て細胞膜がはたらく仕組みを解く

    • Author(s)
      楠見明弘
    • Organizer
      京都大学 再生医科学研究所 第7回公開講演会 「分子から細胞さらに再生医療へ」
    • Place of Presentation
      京都大学(京都)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Organizing principles of the plasma membrane: Three-tiered hierarchical meso-scale domain architecture revealed by single-molecule tracking

    • Author(s)
      楠見明弘
    • Organizer
      Joint Summer Research School in Nanomedicine
    • Place of Presentation
      慶応義塾大学(東京)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 受容体ホモダイマーによるラフト機能創出:1分子イメジングによる研究

    • Author(s)
      楠見明弘
    • Organizer
      第21回日本バイオイメージング学会学術集会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 細胞膜の階層メゾドメイン構造とシグナル変換制御機構:1分子イメージングによる研究

    • Author(s)
      楠見明弘
    • Organizer
      第34回生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      京都大学(京都)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Dynamic organizing principles of the plasma membrane that regulate signal transduction: single-molecule tracking studies

    • Author(s)
      A. Kusumi
    • Organizer
      Departmental Seminar, Dept. of Physiology, University of Otago
    • Place of Presentation
      University of Otago(ニュージーランド)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 細胞膜シグナル伝達を担うメゾスケールドメイン構造:1分子イメージング解析による研究

    • Author(s)
      楠見明弘
    • Organizer
      大阪大学 蛋白質研究所 セミナー 「シグナル伝達と解析技術のあらたな潮流」
    • Place of Presentation
      大阪大学蛋白質研究所(大阪)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks]

    • URL

      http://www.nanobio.frontier.kyoto-u.ac.jp/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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