Protein crystallization at the porous medium
| Project/Area Number |
23657104
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 多孔体 / 界面 / たんぱく質 / 結晶化 / 蛋白質 |
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| Research Abstract |
We investigate the nucleation rate of protein crystals at the interface of solution and porous medium (agarose gel). It was found that 2 ul agarose gel at concentrations of 2.0% (w/v) promoted the number of protein crystals. We also developed a new dispensing system for preparation of the crystallization plates. This technique combined with solution stirring technique improved the crystal quality of a protein complex, thereby solving the crystal structure. The combination with laser irradiation technique demonstrated increase in the nucleation rate. Thus, we propose that a crystallization method using semi-solid agarose is powerful for the screening of crystallization conditions.
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Report
(3 results)
Research Products
(40 results)
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[Journal Article] Spatially precise, soft microseeding of single protein crystals by femtosecond laser ablation2012
Author(s)
H. Yoshikawa, Y. Hosokawa, R. Murai, G. Sazaki, T. Kitatani, H. Adachi, T. Inoue, H. Matsumura, K. Takano, S. Murakami, Y.Mori, and H. Masuhara
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Journal Title
Cryst. Growth Des
Volume: 12(9)
Pages: 4334-4339
Related Report
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[Journal Article] Effects of a forced solution flow on the step advancement on {110} faces of tetragonal lysozyme crystals: direct visualization of individual steps under a forced solution flow2012
Author(s)
M. Maruyama, H. Kawahara, G. Sazaki, S. Maki, Y. Takahashi, H. Y. Yoshikawa, S. Sugiyama, H. Adachi, K. Takano, H. Matsumura, T. Inoue, S. Murakami, and Y. Mori
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Journal Title
Cryst. Growth Des
Volume: 12(6)
Pages: 2856-2863
Related Report
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[Journal Article] Effects of a forced solution flow on the step advancement on {110} faces of tetragonal lysozyme crystals : direct visualization of individual steps under a forced solution flow2012
Author(s)
M.Maruyama, H.Kawahara, G.Sazaki, S.Maki, Y.Takahashi, H.Y.Yoshikawa, S.Sugiyama, H.Adachi, K.Takano, H.Matsumura, T.Inoue, S.Murakami, and Y.Mori
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Journal Title
Crystal Growth & Design
Volume: Vol.12, Issue 6
Issue: 6
Pages: 2856-2863
DOI
Related Report
Peer Reviewed
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[Journal Article] Structure basis for the regulation of glyceraldehyde-3-phosphate dehydro genase activity via the intrinsically disordered protein CP122011
Author(s)
H.Matsumura, A.Kai, T.Maeda, M.Tamoi, A.Satoh, H.Tamura, M.Hirose, T.Ogawa, N.Kizu, A.Wadano, T.Inoue, S.Shigeoka
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Journal Title
Structure
Volume: 19
Issue: 12
Pages: 1846-1854
DOI
Related Report
Peer Reviewed
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