| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
We investigate the nucleation rate of protein crystals at the interface of solution and porous medium (agarose gel). It was found that 2 ul agarose gel at concentrations of 2.0% (w/v) promoted the number of protein crystals. We also developed a new dispensing system for preparation of the crystallization plates. This technique combined with solution stirring technique improved the crystal quality of a protein complex, thereby solving the crystal structure. The combination with laser irradiation technique demonstrated increase in the nucleation rate. Thus, we propose that a crystallization method using semi-solid agarose is powerful for the screening of crystallization conditions.
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