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Characterization of neuronal physiology by second harmonic generation imaging

Research Project

Project/Area Number 23657106
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionKeio University

Principal Investigator

NURIYA Mutsuo  慶應義塾大学, 医学部, 講師 (60453544)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords第二高調波発生 / 2光子顕微鏡 / 神経細胞 / グリア細胞 / SHG / イメージング / 光第二高調波発生 / アストロサイト / 神経
Research Abstract

Despite its unique potential to visualize biological phenomena that can hardly be achieved by other means, applications of second harmonic generation (SHG) imaging to biological researches had remained unexplored. In this study, we tried to overcome this problem by searching for novel SHG dyes as well as new biological targets to apply SHG imaging. We successfully developed new SHG dyes based on unprecedented concepts and found that astrocytes play crucial roles in the brain functions by virtue of their unique cellular characteristics, making astrocytes ideal and important new targets of SHG imaging. Further development of SHG dyes and their applications to SHG imaging of astrocytes are expected to contribute to the development of SHG imaging as well as the cellular understandings of astrocytes in the brain.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (40 results)

All 2014 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 6 results) Presentation (20 results) (of which Invited: 3 results) Book (2 results) Remarks (4 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Astrocytic gap junctional networks suppress cellular damage in an in vitro model of ischemia.2014

    • Author(s)
      Shinotsuka T, Yasui, M and Nuriya M.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 444 Issue: 2 Pages: 171-6

    • DOI

      10.1016/j.bbrc.2014.01.035

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 生体試料におけるSecond Harmonic Generation イメージング2013

    • Author(s)
      塗谷 睦生
    • Journal Title

      生体の科学

      Volume: 64(6) Pages: 545-50

    • Related Report
      2013 Final Research Report
  • [Journal Article] Evaluation of dialkylaminofluorene-based hemicyanine dyes for second harmonic generation imaging by the direct comparison approach.2013

    • Author(s)
      Nuriya, M, Momotake, A, Kimura, Y., Yasui, M., Kasai, H. & Arai, T.
    • Journal Title

      Bull. Chemical Soc. Jpn.

      Volume: 86 Issue: 10 Pages: 1190-1192

    • DOI

      10.1246/bcsj.20130094

    • NAID

      10031201275

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endfeet serve as diffusion-limited subcellular compartments in astrocytes.2013

    • Author(s)
      Nuriya M and Yasui M.
    • Journal Title

      The Journal of Neuroscience.

      Volume: 33 Issue: 8 Pages: 3692-8

    • DOI

      10.1523/jneurosci.3050-12.2013

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diffusion properties of molecules at the blood-brain interface: potential contributions of astrocyte endfeet to diffusion barrier functions.2013

    • Author(s)
      Nuriya M, Shinotsuka T and Yasui M.
    • Journal Title

      Cerebral Cortex

      Volume: 23 Issue: 9 Pages: 2118-2126

    • DOI

      10.1093/cercor/bhs198

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of dialkylaminofluorene-based hemicyanine dyes for second harmonic generation imaging by the direct comparison approach.2013

    • Author(s)
      Nuriya M , Momotake A, Kimura Y, Yasui M, Kasai H and Arai T.
    • Journal Title

      Bull. Chem. Soc. Jpn.

      Volume: 86 Pages: 1190-2

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 生体試料におけるSecond Harmonic Generationイメージング2013

    • Author(s)
      塗谷 睦生
    • Journal Title

      生体の科学

      Volume: 64 Pages: 545-50

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Endfeet serve as diffusion-limited subcellular compartments in astrocytes.2013

    • Author(s)
      Nuriya M and Yasui M
    • Journal Title

      The Journal of Neuroscience

      Volume: 33(8) Pages: 3692-3698

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 神経細胞における電位情報伝達のイメージング2012

    • Author(s)
      塗谷 睦生
    • Journal Title

      レーザー研究

      Volume: 40(4) Pages: 224-9

    • NAID

      10030510431

    • Related Report
      2013 Final Research Report
  • [Journal Article] 2 光子顕微鏡の神経科学研究への応用 : 分子から個体までを繋ぐ病態解明への新たな光として2012

    • Author(s)
      塗谷 睦生
    • Journal Title

      実験医学増刊号「心とからだのクロストーク精神・神経疾患のメディカルサイエンス」

      Volume: 30(13) Pages: 107-11

    • Related Report
      2013 Final Research Report
  • [Journal Article] 神経細胞における電位情報伝達のイメージング2012

    • Author(s)
      塗谷睦生
    • Journal Title

      レーザー研究

      Volume: 40 (4) Pages: 224-229

    • NAID

      10030510431

    • Related Report
      2012 Research-status Report
  • [Journal Article] 2光子顕微鏡の神経科学研究への応用:分子から個体までを繋ぐ病態解明への新たな光として2012

    • Author(s)
      塗谷睦生
    • Journal Title

      実験医学

      Volume: 30 (13) Pages: 107-111

    • Related Report
      2012 Research-status Report
  • [Presentation] アストロサイトのギャップジャンクションによるネットワークは虚血ダメージを軽減する2014

    • Author(s)
      篠塚崇徳, 安井正人, 塗谷睦生
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2014-03-19
    • Related Report
      2013 Final Research Report
  • [Presentation] アストロサイト足突起における情報伝達様式 ~神経血管連関の細胞生物学的基盤の理解に向けて~2014

    • Author(s)
      塗谷睦生
    • Organizer
      第2回Neurovascular Unit 研究会
    • Place of Presentation
      東京
    • Year and Date
      2014-01-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 光第二高調波イメージングの生命科学研究への応用 ~神経細胞における定量的膜電位イメージング~2013

    • Author(s)
      塗谷睦生
    • Organizer
      応用物理学会・量子エレクトロニクス研究会
    • Place of Presentation
      軽井沢
    • Year and Date
      2013-12-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Astrocytic gap junctional networks suppress cellular damages in an in vitro model of ischemia2013

    • Author(s)
      Shinotsuka T, Yasui M, Nuriya M
    • Organizer
      Annual Meeting of Society for Neuroscience
    • Place of Presentation
      San Diego (USA)
    • Year and Date
      2013-11-13
    • Related Report
      2013 Final Research Report
  • [Presentation] アストロサイトのギャップジャンクションによるネットワークは虚血ダメージを軽減する2013

    • Author(s)
      篠塚崇徳, 塗谷睦生, 安井正人
    • Organizer
      日本神経科学学会年会
    • Place of Presentation
      京都
    • Year and Date
      2013-06-21
    • Related Report
      2013 Final Research Report
  • [Presentation] アストロサイト細胞内外における分子動態解析が明らかにするグリア・血管相互作用様式2013

    • Author(s)
      塗谷睦生
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] アストロサイト細胞内外における分子動態解析が明らかにするグリア・血管相互作用様式2013

    • Author(s)
      塗谷睦生
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 血液脳関門での物質拡散抑制機能に対しるアストロサイト足突起の寄与2012

    • Author(s)
      塗谷睦生
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 血液脳関門での物質拡散抑制機能に対しるアストロサイト足突起の寄与2012

    • Author(s)
      塗谷睦生、安井正人
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] 光第二高調波イメージングの生理学研究への応用2011

    • Author(s)
      塗谷睦生
    • Organizer
      平成23年度生理研研究会「神経活動の光操作」
    • Place of Presentation
      岡崎
    • Year and Date
      2011-09-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of two-photon microscopy in physiology2011

    • Author(s)
      Mutsuo Nuriya
    • Organizer
      7th Congress of the Federation of Asian and Oceanian Physiological Societies
    • Place of Presentation
      台湾
    • Year and Date
      2011-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 第二高調波イメージングの生理学研究への応用2011

    • Author(s)
      塗谷睦生
    • Organizer
      日本顕微鏡学会第67回学術講演会
    • Place of Presentation
      福岡
    • Year and Date
      2011-05-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 第二高調波イメージングの生理学研究への応用2011

    • Author(s)
      塗谷睦生
    • Organizer
      日本顕微鏡学会第67回学術講演会(招待講演)
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Application of two-photon microscopy in physiology2011

    • Author(s)
      Mutsuo Nuriya
    • Organizer
      7th Congress of the Federation of Asian and Oceanian Physiological Societies(招待講演)
    • Place of Presentation
      台湾
    • Related Report
      2011 Research-status Report
  • [Presentation] 光第二高調波イメージングの生理学研究への応用2011

    • Author(s)
      塗谷睦生
    • Organizer
      平成23年度生理研研究会「神経活動の光操作」(招待講演)
    • Place of Presentation
      岡崎カンファレンスセンター(愛知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 光第二高調波イメージングの生命科学研究への応用 ~神経細胞における定量的膜電位イメージング~

    • Author(s)
      塗谷 睦生
    • Organizer
      応用物理学会・量子エレクトロニクス研究会
    • Place of Presentation
      軽井沢上智大学セミナーハウス
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] アストロサイト足突起における情報伝達様式 ~神経血管連関の細胞生物学的基盤の理解に向けて~

    • Author(s)
      塗谷 睦生
    • Organizer
      Neurovascular Unit研究会
    • Place of Presentation
      慶應義塾大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] アストロサイトのギャップジャンクションによるネットワークは虚血ダメージを軽減する

    • Author(s)
      篠塚崇徳, 塗谷睦生, 安井正人
    • Organizer
      日本神経科学会年会
    • Place of Presentation
      京都国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Astrocytic gap junctional networks suppress cellular damages in an in vitro model of ischemia

    • Author(s)
      Shinotsuka T, Yasui M, Nuriya M
    • Organizer
      Annual Meeting of the Society for Neuroscience (U.S.)
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] アストロサイトのギャップジャンクションによるネットワークは虚血ダメージを軽減する

    • Author(s)
      篠塚崇徳, 安井正人, 塗谷睦生
    • Organizer
      日本薬理学会年会
    • Place of Presentation
      仙台国際センター
    • Related Report
      2013 Annual Research Report
  • [Book] Imaging membrane potential with second harmonic generation. A book chapter in "Second Harmonic Generation Imaging" Taylor and Francis2013

    • Author(s)
      Nuriya M & Yuste R
    • Related Report
      2013 Final Research Report
  • [Book] Second Harmonic Generation Imaging2013

    • Author(s)
      Nuriya M and Yuste R
    • Total Pages
      440
    • Publisher
      Taylor and Francis
    • Related Report
      2013 Annual Research Report
  • [Remarks] 塗谷グループのホームページ

    • URL

      http://web.keio.jp/~nuriya/main/homepage.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 塗谷グループのホームページ

    • URL

      http://web.keio.jp/~nuriya/main/homepage.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 塗谷グループのホームページ

    • URL

      http://web.keio.jp/~nuriya/main/homepage.html

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://web.keio.jp/~nuriya/main/homepage.html

    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] 光第二高調波発生化合物, 光第二高調波発生色素組成物及び細胞検査方法2013

    • Inventor(s)
      塗谷睦生, 安井正人, 新井達郎, 百武篤也, 福嶋瞬
    • Industrial Property Rights Holder
      学校法人慶應義塾
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-109179
    • Filing Date
      2013-05-23
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 光第二高調波発生化合物、光第二高調波発生色素組成物及び細胞検査方法2013

    • Inventor(s)
      塗谷睦生、安井正人、新井達郎、百武篤也、福嶋瞬
    • Industrial Property Rights Holder
      塗谷睦生、安井正人、新井達郎、百武篤也、福嶋瞬
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-05-23
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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