Development of temperature sensing fluorescent protein for in vivo thermal imaging induced by infrared laser irradiation to cells

• Project/Area Number: 23657109
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: National Institute for Basic Biology
• Principal Investigator: KAMEI Yasuhiro 基礎生物学研究所, 生物機能解析センター, 特任准教授 (70372563)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: バイオイメージング / 遺伝子発現 / 蛍光タンパク質 / 赤外レーザー / 温度プローブ / メダカ

Research Abstract

Single-cell gene induction is a powerful method for gene function and cell fate analysis. Infrared laser evoked gene operator method can enable this by utilizing heat shock response system to induce a target gene in the single cell of transgenic animals or plants which carries the target gene driven by heat shock promoter. However, because excess heating results in cell damage, we developed temperature-sensitive fluorescent protein and real time temperature sensing microscope system to improve the method

Research Products

• [Journal Article] Trunk exoskeleton in teleosts is mesodermal in origin. (2013)
  • Author(s): Atsuko Shimada
  • Journal Title: Nature Communications Volume: 4 Issue: 1 Pages: 1-8
  • DOI: 10.1038/ncomms2643
  • Peer Reviewed / Open Access
• [Journal Article] A heat-inducible CRE/LOXP gene induction system in medaka (2013)
  • Author(s): Kobayashi K, Kamei Y, Kinoshita M, Czerny T, Tanaka M
  • Journal Title: Genesis Volume: 51 Pages: 59-67
  • Peer Reviewed
• [Journal Article] Application of Infrared Laser to the Zebrafish Vascular System: Gene Induction, Tracing, and Ablation of Single Endothelial Cells. (2013)
  • Author(s): Kimura E, Deguchi T, Kamei Y, Shoji W, Yuba S, Hitomi J
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 33 Issue: 6 Pages: 1264-1270
  • DOI: 10.1161/atvbaha.112.300602
  • Peer Reviewed
• [Journal Article] ATF6a/b-mediated adjustment of ER chaperone levels is essential for development of the notochord in medaka fish. (2013)
  • Author(s): Ishikawa, T., Okada, T., Ishikawa-Fujisawa, T., Todo, T., Kamei, Y., Shigenobu, S., Tanaka, M., Saito, T.L., Yoshimura, J., Morishita, S., Toyoda, A., Sakaki, Y., Taniguchi, Y., Takeda, S. and Mori, K.
  • Journal Title: Mol. Biol. Cell. Volume: 24 Issue: 1 Pages: 1387-1395
  • DOI: 10.1002/dvg.22348
  • Peer Reviewed
• [Journal Article] Controlled Cre/loxP site-specific Recombination in the Developing Brain in Medaka fish, Oryzias latipes. (2013)
  • Author(s): Okuyama T, Isoe Y, Hoki M, Suehiro Y, Yamagishi G, Naruse K, Kinoshita M, Kamei Y, Shimizu A, Kubo T and Takeuchi H.
  • Journal Title: PLoS One Volume: in press
  • Peer Reviewed
• [Journal Article] Application of infrared laser to the zebrafish vascular system: gene induction, tracing, and ablation of single endothelal cells
  • Author(s): Kimura E, Deguchi T, Kamei Y, Shoji W, Yuba S and Hitomi J
  • Journal Title: Arterioscler. Thromb. Vasc. Biol
  • Peer Reviewed
• [Journal Article] Controlled Cre/loxP site-specific recombination in the developing brain in medaka fish, Oryzias latipes
  • Author(s): Okuyama T, Isoe Y, Hoki M, Suehiro Y,Yamagishi G, Naruse K, Kinoshita M, Kamei Y, Shimizu A, Kubo T and Takeuchi H
  • Journal Title: PLoS One
  • Peer Reviewed
• [Presentation] Applications of IR-LEGO (infrared laser-evoked gene operator) to the model plant as collaborative research projects in NIBB (2013)
  • Author(s): 亀井保博(他9名)
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学(岡山県)
  • Year and Date: 2013-03-22
• [Presentation] Applications of IR-LEGO (infrared laser-evoked gene operator) to the model plant as collaborative research projects in NIBB (2013)
  • Author(s): 亀井保博（他9名）
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学（岡山県）
• [Presentation] Infrared Laser Mediated Single-Cell Gene Induction Method: Development and Application of IR-LEGO System (2012)
  • Author(s): Misako Saida-Taniguchi, Takuya Kaneko, Atsuko Shimada, Hiroyuki Takeda and Yasuhiro Kamei
  • Organizer: 18th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 京都大学 (京都 府)
  • Year and Date: 2012-09-22
• [Presentation] Development of IR laser-mediated gene induction system (2012)
  • Author(s): Yasuhiro Kamei
  • Organizer: 2^<nd> NUS-TLL-NIBB International Practical Course-Genetics, Genomics and Imaging in Zebrafish and Medaka
  • Place of Presentation: National Univ. of Singapore (シンガポール)招待講演
  • Year and Date: 2012-07-26
• [Presentation] 赤外レーザーによる1 細胞遺伝子発現法 (2012)
  • Author(s): 亀井保博, 浦和博子, 岡田清孝
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-16
• [Presentation] Infrared Laser Mediated Single-Cell Gene Induction Method: Development and Application of IR-LEGO System (2012)
  • Author(s): Misako Saida-Taniguchi, Takuya Kaneko, Atsuko Shimada, Hiroyuki Takeda and Yasuhiro Kamei
  • Organizer: Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 京都大学（京都府）
• [Presentation] Development of IR laser-mediated gene induction system (2012)
  • Author(s): Yasuhiro Kamei
  • Organizer: 2nd NUS-TLL-NIBB International Practical Course-Genetics, Genomics and Imaging in Zebrafish and Medaka
  • Place of Presentation: Univ. of Singapore (シンガポール)
  • Invited
• [Presentation] 赤外レーザーによる1細胞遺伝子発現法 (2012)
  • Author(s): 亀井保博、浦和博子、岡田清孝
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都産業大学（京都府）
• [Presentation] 赤外レーザーによる遺伝子発現システム(IR-LEGO)の様々 なモデル生物への応用 (2011)
  • Author(s): 亀井保博,(他16名)
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜 (神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] Infrared laser mediated single-cell gene induction method: Development and application of IR-LEGO system. 1st Strategic meeting for medaka research method: Development and application of IR-LEGO system (2011)
  • Author(s): Yasuhiro Kamei
  • Organizer: 1^<st> Strategic meeting for medaka research
  • Place of Presentation: 岡崎カンファレンスセンター(愛知県)
  • Year and Date: 2011-11-23
• [Presentation] 赤外レーザーによる遺伝子発現システム（IR-LEGO）の様々なモデル生物への応用 (2011)
  • Author(s): 亀井保博、浦和博子、山本和彦、兼子拓也、木村英二、出口友則、弓場俊輔、北野健、尾田正二、三谷啓志、蟹江裕太、斎田-谷口美佐子、鈴木友美、長谷あきら、島田敦子、武田洋幸、岡田清孝
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Infrared laser mediated single-cell gene induction method: Development and appliation of IR-LEGO system (2011)
  • Author(s): Yasuhiro Kamei
  • Organizer: 1st Strategic meeting for medaka research
  • Place of Presentation: 岡崎カンファレンスセンター（愛知県）
• [Book] 生物機能モデルと新しいリソース・リサーチツール (2011)
  • Author(s): 亀井保博(他111 名)
  • Publisher: エルアイシー出版
• [Book] 生物機能モデルと新しいリソース・リサーチツール (2011)
  • Author(s): 亀井保博（他111名）
  • Total Pages: 671
  • Publisher: エルアイシー
• [Remarks] ホームページ 研究内容ならびに研究成果は研究室HPに随時掲載
  • URL: http://www.nibb.ac.jp/lspectro/kamei.htm#original
• [Remarks] プレスリリース Nature Communications 誌での論文発表に 関しては東京大学ならびに基礎生物学研究所からプレスリリースを実施(2013 年3 月 28 日)。
• [Remarks] Arterioscler. Thromb. Vasc. Biol.誌での論文発表に関しては岩手医科大学・基礎生物学研究所共同プレスリリースを実施(2013年5月31日予定)。
• [Remarks] 技術講習会局所遺伝子発現技術(IR-LEGO 法)はトレーニングコースとして、シンガポール大学 (2012年7月)と、基礎生物学研究所(2012年10月)で実施した。また、2013年6月に中部地区技術職員向けにも実施予定。
• [Remarks] 基礎生物学研究所 光学解析室 亀井グループ研究紹介
  • URL: http://www.nibb.ac.jp/lspectro/kamei.htm
• [Remarks] 基礎生物学研究所 プレスリリース メダカのウロコが証す骨の起源
  • URL: http://www.nibb.ac.jp/pressroom/news/2013/03/28.html