Development of temperature sensing fluorescent protein for in vivo thermal imaging induced by infrared laser irradiation to cells
| Project/Area Number |
23657109
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
KAMEI Yasuhiro 基礎生物学研究所, 生物機能解析センター, 特任准教授 (70372563)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | バイオイメージング / 遺伝子発現 / 蛍光タンパク質 / 赤外レーザー / 温度プローブ / メダカ |
|---|
| Research Abstract |
Single-cell gene induction is a powerful method for gene function and cell fate analysis. Infrared laser evoked gene operator method can enable this by utilizing heat shock response system to induce a target gene in the single cell of transgenic animals or plants which carries the target gene driven by heat shock promoter. However, because excess heating results in cell damage, we developed temperature-sensitive fluorescent protein and real time temperature sensing microscope system to improve the method
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] ATF6a/b-mediated adjustment of ER chaperone levels is essential for development of the notochord in medaka fish.2013
Author(s)
Ishikawa, T., Okada, T., Ishikawa-Fujisawa, T., Todo, T., Kamei, Y., Shigenobu, S., Tanaka, M., Saito, T.L., Yoshimura, J., Morishita, S., Toyoda, A., Sakaki, Y., Taniguchi, Y., Takeda, S. and Mori, K.
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Journal Title
Mol. Biol. Cell.
Volume: 24
Issue: 1
Pages: 1387-1395
DOI
Related Report
Peer Reviewed
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[Journal Article] Controlled Cre/loxP site-specific Recombination in the Developing Brain in Medaka fish, Oryzias latipes.2013
Author(s)
Okuyama T, Isoe Y, Hoki M, Suehiro Y, Yamagishi G, Naruse K, Kinoshita M, Kamei Y, Shimizu A, Kubo T and Takeuchi H.
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Journal Title
PLoS One
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Controlled Cre/loxP site-specific recombination in the developing brain in medaka fish, Oryzias latipes
Author(s)
Okuyama T, Isoe Y, Hoki M, Suehiro Y,Yamagishi G, Naruse K, Kinoshita M, Kamei Y, Shimizu A, Kubo T and Takeuchi H
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Journal Title
Related Report
Peer Reviewed
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