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The analysis of cell-to-cell communications by re-constructive artificial networks of neurons using non invasive cell collection techniques

Research Project

Project/Area Number 23657111
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research Institution公益財団法人神奈川科学技術アカデミー (2012)
Kanagawa Academy of Science and Technology (2011)

Principal Investigator

TERAZONO Hideyuki  公益財団法人神奈川科学技術アカデミー, オンチップ・セロミクスプロジェクト, 研究員 (30398143)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords脳 / 神経系の情報処理/ 細胞間コミュニケーション / 人工神経回路 / マイクロパターニング / アガロース / 細胞外電位 / 神経細胞 / 神経ネットワーク / 多電極同時計測
Research Abstract

We have been studying the living system by assembling artificial tissues composed of single cells as a minimum functional unit. Along this research theme, we have studied cell-to-cell communications by controlling the spatial pattern of cell networks. In the course of research, our purpose is to understand information processing of neuron networks. In the results, we developed a non-invasive culturing and cell sorting method for primary neurons using alginate thin sheet. Usually, neurons cannot be collected selectively because neurons form spherical so that cannot be distinguished by floating condition.Moreover, after growth, if there are injured by protease, neurons will be died. The method we developed allows each single particular neuron to collect after axon or neurites are growth. By combine our method and the agarose micro-patterning techniques to make neuronal network artificially, neuronal networks using actual neurons can be constructed artificially and we will demonstrate the principle how neuronal networks work.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (10 results)

All 2012 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer2012

    • Author(s)
      Terazono H, Kim H, Hayashi M, Hattori A, Nomura F, Kaneko T, Yasuda K
    • Journal Title

      PLoS One

      Volume: 7(8)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer2012

    • Author(s)
      Hideyuki Terazono
    • Journal Title

      PLoS One

      Volume: 7(8) Issue: 8 Pages: e42485-e42485

    • DOI

      10.1371/journal.pone.0042485

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Toward Quasi-In Vivo from In Vitro Assay (V): Noninvasive Precise Purification of Ventricular Cells from Mixture of Differentiated Human Stem Cell-Derived Cardiomyocytes Using Spot Digestion of Double Alginate Layers on a Multielectrode Array Chip2012

    • Author(s)
      Hideyuki Terazono、Hyonchol Kim、Akihiro Hattori、Tomoyuki Kaneko、Kenji Yasuda
    • Organizer
      Safety Pharmacology Society (SPS2012)
    • Place of Presentation
      Phoenix convention center, Arizona, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] A Non-destructive Culturing and Cell Sorting Method for Cardiomyocytes and Neurons Using an Alginate Layer2012

    • Author(s)
      寺薗英之、金賢徹、服部明弘、野村典正、金子智行、安田賢二
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Final Research Report
  • [Presentation] Toward Quasi-In Vivo from In Vitro Assay (V): Noninvasive Precise Purification of Ventricular Cells from Mixture of Differentiated Human Stem Cell-Derived Cardiomyocytes Using Spot Digestion of Double Alginate Layers on a Multielectrode Array Chip2012

    • Author(s)
      Hideyuki Terazono
    • Organizer
      Safety Pharmacology Society (SPS2012)
    • Place of Presentation
      Phoenix, Arizona, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] A Non-destructive Culturing and Cell Sorting Method for Cardiomyocytes and Neurons Using an Alginate Layer2012

    • Author(s)
      寺薗 英之
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学 東山キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] CONSTRUCTION AND ANALYSIS OF AN ARTIFICIAL NEURONAL NETWORK USING A NEURON-COLLECTING2011

    • Author(s)
      Hideyuki Terazono, Hyonchol Kim, Masahito Hayashi, Akihiro Hattori, Hiroyuki Takei, Kenji Yasuda
    • Organizer
      MICRO-PATTERNING METHOD BASED ON A MULTI-ELECTRODE ARRAY SYSTEM NCTA2011
    • Place of Presentation
      Universite Paris-Est Creteil (UPEC)、Paris, France
    • Related Report
      2012 Final Research Report
  • [Presentation] Construction of an artificial neuronal network and electrophysiological measurement with a selective collection method of cultured primary neurons2011

    • Author(s)
      Hideyuki Terazono, Hyonchol Kim, Masahito Hayashi, Akihiro Hattori, Kenji Yasuda
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 細胞配置装置および方法2012

    • Inventor(s)
      寺薗英之、安田賢二、金賢徹、服部明弘
    • Industrial Property Rights Holder
      寺薗英之、安田賢二、金賢徹、服部明弘
    • Industrial Property Number
      2012-077196
    • Filing Date
      2012
    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] 1細胞レベルからの培養細胞の非侵襲的剥離回収装置および方法2010

    • Inventor(s)
      安田賢二、寺薗英之、服部明弘
    • Industrial Property Rights Holder
      神奈川科学技術アカデミー・東京医科歯科大学・株式会社オンチップ・セロミクスコンソーシアム
    • Filing Date
      2010-02-16
    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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