Development of a novel method detecting interaction between proteins and DNAs

• Project/Area Number: 23657119
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: ITO Yasuhiro 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品バイオテクノ ロジー研究領域, 主任研究員 (90353987)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 遺伝学 / 核酸 / 蛋白質 / 発現制御 / 分子認識 / 転写因子 / DNA / 相互作用 / 分子間相互作用

Research Abstract

To develop a sensitive method detecting interaction of proteins with their binding DNAs, we applied a real-time PCR based methods with probe DNA-conjugated antibodies to a protein and also the target DNA conjugated another probe DNA. We detected interaction between a protein and the target DNAs by the method, but the sensitivity was much lower than that by the other authentic method. Thus, additional improvement is required for the practical use of this new method.