Construction of system that controls vesicular transport reaction in living cells by light irradiation
| Project/Area Number |
23657123
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATO Ken 東京大学, 大学院・総合文化研究科, 准教授 (00303602)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 小胞輸送 / 小胞体 / コートタンパク質 / フォトトロピン / 低分子量GTPase / 出芽酵母 |
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| Research Abstract |
To control the vesicular transport reaction by light irradiation in living yeast cells, PHOT1 that induce light-dependent conformational change was fused to the factor that mediates this reaction. Sar1 was selected as a target for controlling ER-to-Golgi vesicular transport, and although PHOT1-Sar1-mCherry fusion protein was expressed in yeast cells, light-dependent regulation has not been observed so far.
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Report
(3 results)
Research Products
(10 results)
