Construction of system that controls vesicular transport reaction in living cells by light irradiation

• Project/Area Number: 23657123
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: SATO Ken 東京大学, 大学院・総合文化研究科, 准教授 (00303602)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 小胞輸送 / 小胞体 / コートタンパク質 / フォトトロピン / 低分子量GTPase / 出芽酵母

Research Abstract

To control the vesicular transport reaction by light irradiation in living yeast cells, PHOT1 that induce light-dependent conformational change was fused to the factor that mediates this reaction. Sar1 was selected as a target for controlling ER-to-Golgi vesicular transport, and although PHOT1-Sar1-mCherry fusion protein was expressed in yeast cells, light-dependent regulation has not been observed so far.

Research Products

• [Journal Article] COPII machinery cooperates with ER-localized Hsp40 to sequester misfolded membrane proteins into ER-associated compartment. (2013)
  • Author(s): Kakoi, S., Yorimitsu, T., Sato, K.
  • Journal Title: Mol Biol Cell Volume: 24 Issue: 5 Pages: 633-642
  • DOI: 10.1091/mbc.e12-08-0639
  • Peer Reviewed
• [Journal Article] Involvement of the penta-EF-hand protein Pef1p in the Ca2+-dependent regulation of COPII subunit assembly in Saccharomyces cerevisiae (2012)
  • Author(s): Yoshibori, M., Yorimitsu, T., Sato, K.
  • Journal Title: PLoS ONE Volume: 7 Issue: 7 Pages: e40765-e40765
  • DOI: 10.1371/journal.pone.0040765
  • Peer Reviewed
• [Journal Article] Insight into structural and regulatory roles of Sec16 in COPII vesicle formation at ER exit sites (2012)
  • Author(s): T. Yorimitsu and K. Sato
  • Journal Title: Mol. Biol. Cell Volume: 23 Issue: 15 Pages: 2930-2942
  • DOI: 10.1091/mbc.e12-05-0356
  • Peer Reviewed
• [Presentation] 再構成と可視化による膜交通の分子機構の解明 (2012)
  • Author(s): 佐藤健
  • Organizer: 公開シンポジウム「タンパク質の細胞内交通整理」
  • Place of Presentation: 東京大学伊藤謝恩ホール(招待講演)
  • Year and Date: 2012-09-10
• [Presentation] 再構成と可視化による膜交通の分子機構の解明
  • Author(s): 佐藤 健
  • Organizer: 公開シンポジウム「タンパク質の細胞内交通整理」
  • Place of Presentation: 東京大学 伊藤謝恩ホール
  • Invited
• [Presentation] 再構成と分子イメージングによる輸送小胞形成の分子メカニズム解析
  • Author(s): 佐藤 健
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク
• [Presentation] 小胞体における輸送小胞形成の時空間制御
  • Author(s): 佐藤 健
  • Organizer: 第84回日本生化学会大会(招待講演)
  • Place of Presentation: 国立京都国際会館
• [Presentation] Sec16 function in COPII vesicle formation at ER
  • Author(s): 依光朋宏、佐藤 健
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
• [Book] 化学と生物 「小胞体から出芽する輸送小胞に輸送基質が濃縮される仕組み」 (2012)
  • Author(s): 佐藤 健
  • Total Pages: 3
  • Publisher: 学会出版センター
• [Remarks]
  • URL: http://kensato01.c.u-tokyo.ac.jp/~kensato/index.html