Mechanisms of morphological changes of mitochondria during neural differentiation and under disease condition by using cell-resealing technique
| Project/Area Number |
23657124
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | シグナル伝達 / 神経科学 / 細胞･組織 / セミインタクト細胞 / 細胞・組織 / 発生・分化 |
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| Research Abstract |
We established a neural cell line N14.5-Tom5 cells, in which mitochondrial protein GFP-Tom5 is stably expressed. By using N14.5-Tom5, we found that activation of GSK3beta or Akt was involved in mitochondrial fission during neural differentiation, and identified the phosphorylation of Drp1, dynamin-related GTPase, by GSK3beta regulated the fission. Interestingly, the phosphorylation of Drp1 is reported be required for the mitochondrial fission at the onset of mitosis in mammalian cells. Next, we constructed model cells that replicated the conditions in neurons of Alzheimer's disease by using the resealing-cell technique and cytosol prepared from ApoE(-/-) mouse. As a result, we found that inactivity of Akt in the model cells caused the elongation of mitochondria and resulted in depressed mitochondrial function. Collectively, the persistent phosphorylation of both GSK3beta and Akt plays a crucial role for the maintaining normal mitochondrial morphology and function.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] PKCd and e regulate the morphological integrity of the ER-Golgi intermediate compartment (ERGIC) but not the anterograde and retrograde transports via the Golgi apparatus2012
Author(s)
Sugawara, T., Nakatsu, D., Kii, H., Maiya, N., Adachi, A., Yamamoto, A., Kano, F., Murata, M
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Journal Title
Biochem. Biophys. Acta (Molecular Cell Research)
Volume: 1823(4)
Pages: 861-875
Related Report
Peer Reviewed
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[Journal Article] KCδ and ε regulate the morphological integrity of the ER-Golgi intermediate compartment (ERGIC) but not the anterograde and retrograde transports via the Golgi apparatus.2012
Author(s)
Sugawara, T., Nakatsu, D., Kii, H., Maiya, N., Adachi, A., Yamamoto, A., Kano, F., Murata, M.
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Journal Title
Biochem. Biophys. Acta (Molecular Cell Research)
Volume: 1823
Pages: 861-875
Related Report
Peer Reviewed
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[Journal Article] PKCd and e regulate the morphological integrity of the ER-Golgi intermediate compartment (ERGIC) but not the anterograde and retrograde transports via the Golgi apparatus.2012
Author(s)
Sugawara, T., Nakatsu, D., Kii, H., Maiya, N., Adachi, A., Yamamoto, A., Kano, F., Murata, M.
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Journal Title
Biochem. Biophys. Acta (Molecular Cell Research)
Volume: 1823
Pages: 861-875
Related Report
Peer Reviewed
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[Journal Article] The small GTPase Cdc42 modulates the number of exocytosis-competent dense-core vesicles in PC12 cells.2012
Author(s)
Sato, M.,Kitaguchi, T., Numano, R., Ikematsu, K., Kakeyama, M., Murata, M., Sato, K., Tsuboi, T.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 420
Pages: 417-421
Related Report
Peer Reviewed
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[Journal Article] Localization of mature Neprilysin in lipid rafts.2011
Author(s)
Sato, K., Tanabe, C.,Yonemura, Y., Watahiki, H., Zhao, Y., Yagishita, S., Ebina, M., Suo, S., Futai, U., Murata, M., Ishiura, S.
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Journal Title
J. Neurosci. Res.
Volume: 90
Pages: 870-878
Related Report
Peer Reviewed
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