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Development of high-resolution pulse-chase method by use of click chemical reaction

Research Project

Project/Area Number 23657126
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionNagoya University

Principal Investigator

FUJIMOTO Toyoshi  名古屋大学, 医学系研究科, 教授 (50115929)

Co-Investigator(Kenkyū-buntansha) SUZUKI Michitaka  名古屋大学, 医学系研究科, 助教 (80456649)
OZATO Nami  名古屋大学, 医学系研究科, COE 特任助教 (60547454)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords膜脂質 / 凍結割断レプリカ標識法 / クリック反応 / 電子顕微鏡 / 凍結割断レプリカ
Research Abstract

Cells cultured with precursor molecules bearing an alkyl or an azido group were quick-frozen and freeze-fracture replicas were subjected to click chemical reaction to label membrane lipids and proteins for electron microscopy. The labeling for phosphatidylcholine increased in a time-dependent manner after the precursor treatment. In budding yeast, the labeling density in the endoplasmic reticulum, nuclear membrane, mitochondrion, and vacuole was equivalent in the two membrane leaflets, whereas that in the plasma membrane showed clear asymmetry.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (5 results)

All 2013 2011 Other

All Presentation (4 results) Remarks (1 results)

  • [Presentation] 電子顕微鏡による膜脂質ドメインの解析2013

    • Author(s)
      藤本豊士
    • Organizer
      理研コロキウム
    • Place of Presentation
      和光
    • Year and Date
      2013-02-22
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] ホスファチジルコリンの代謝的ラベル化と非対称性分布解析2013

    • Author(s)
      伊吉祥平、藤本豊士
    • Organizer
      第118回日本解剖学会総会・学術集会
    • Place of Presentation
      高松
    • Related Report
      2012 Final Research Report
  • [Presentation] ホスファチジルコリンの代謝的ラベル化と非対称性分布解析2013

    • Author(s)
      伊吉祥平、藤本豊士
    • Organizer
      第118回日本解剖学会総会・全国学術大会
    • Place of Presentation
      高松
    • Related Report
      2012 Annual Research Report
  • [Presentation] Nanoscale distribution of phospholipids observed by electron microscopy2011

    • Author(s)
      Toyoshi Fujimoto
    • Organizer
      New Aspect of Phospholipid Biology and Medicine 2011(招待講演)
    • Place of Presentation
      博多(ホテル・ルイガンス)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.med.nagoya-u.ac.jp/cel-bio/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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