Challenge to dogma in autophagy

• Project/Area Number: 23657129
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: YOSHIMORI Tamotsu 大阪大学, 大学院・生命機能研究科, 教授 (60191649)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: オートファジー / 標的認識 / Atg / 選択的オートファジー / LC3 / ユビキチン / ＬＣ3

Research Abstract

Autophagy is intracellular bulk degradation system. While recent progress in autophagy field has expanded our knowledge of the process, there are some dogma, which are widely believed but examined well. Here, we analyzed carefully one of them, a believed model for target recognition in selective autophagy. We showed that interaction between adopter proteins and LC3 is not enough to explain the recognition and there is a case that target itself is not recognized.

Research Products

• [Journal Article] Autophagy Plays a Significant Role in Determining Skin Color by Regulating Melanosome Degradation in Keratinocytes (2013)
  • Author(s): Murase D, Hachiya A, Takano K, Hicks R, Visscher MO, Kitahara T, Hase T, Takema Y, Yoshimori T
  • Journal Title: J Invest Dermatol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Autophagosomes form at ER-mitochondria contact sites (2013)
  • Author(s): Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Amano A*, Yoshimori T*
  • Journal Title: Nature Volume: 495(7441) Pages: 389-393
  • Peer Reviewed
• [Journal Article] Impaired autophagy by soluble endoglin, under physiological hypoxia in early pregnant period, is involved in poor placentation in preeclampsia (2013)
  • Author(s): Nakashima A, Yamanaka-Tatematsu M, Fujita N, Koizumi K, Shima T, Yoshida T, Nikaido T, Okamoto A, Yoshimori T, Saito S
  • Journal Title: Autophagy Volume: 9(3) Pages: 303-316
  • Peer Reviewed
• [Journal Article] Critical roles for lipomannan and lipoarabinomannan in cell wall integrity of mycobacteria and pathogenesis of tuberculosis (2013)
  • Author(s): Fukuda T, Matsumura T, Ato M, Hamasaki M, Nishiuchi Y, Murakami Y, Maeda Y, Yoshimori T, Matsumoto S, Kobayashi K, Kinoshita T, Morita YS
  • Journal Title: Mbio Volume: 4(1)
  • Peer Reviewed
• [Journal Article] Autophagosomes form at ER.mitochondria contact sites (2013)
  • Author(s): Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
  • Journal Title: Nature Volume: 495 Issue: 7441 Pages: 389-93
  • DOI: 10.1038/nature11910
  • Peer Reviewed
• [Journal Article] Beclin-1-interacting autophagy protein Atg14L targets the SNARE-associated protein Snapin to coordinate endocytic trafficking (2012)
  • Author(s): Kim HJ, Zhong Q, Sheng ZH, Yoshimori T, Liang C, Jung JU
  • Journal Title: J Cell Sci Volume: 125(Pt 20) Pages: 4740-4750
  • Peer Reviewed
• [Journal Article] Autophagy creates a CTL epitope that mimics tumor-associated antigens (2012)
  • Author(s): Demachi-Okamura A, Torikai H, Akatsuka Y, Miyoshi H, Yoshimori T, Kuzushima K
  • Journal Title: PLoS One Volume: 7(10)
  • Peer Reviewed
• [Journal Article] Inhibition of autophagy potentiates the antitumor effect of the multikinase inhibitor sorafenib in hepatocellular carcinoma (2012)
  • Author(s): Shimizu S, Takehara T, Hikita H, Kodama T, Tsunematsu H, Miyagi T, Hosui A, Ishida H, Tatsumi T, Kanto T, Hiramatsu N, Fujita N, Yoshimori T, Hayashi N
  • Journal Title: Int J Cancer Volume: 131(3) Pages: 548-557
  • Peer Reviewed
• [Journal Article] Bornavirus closely associates and segregates with host chromosomes to ensure persistent intranuclear infection (2012)
  • Author(s): Matsumoto Y, Hayashi Y, Omori H, Honda T, Daito T, Horie M, Ikuta K, Fujino K, Nakamura S, Schneider U, Chase G, Yoshimori T, Schwemmle M, Tomonaga K
  • Journal Title: Cell Host Microbe Volume: 11(5) Pages: 492-503
  • NAID: 120004161070
  • Peer Reviewed
• [Journal Article] Jo EK.Host cell autophagy activated by antibiotics is required for their effective antimycobacterial drug action (2012)
  • Author(s): Kim JJ, Lee HM, Shin DM, Kim W, Yuk JM, Jin HS, Lee SH, Cha GH, Kim JM, Lee ZW, Shin SJ, Yoo H, Park YK, Park JB, Chung J, Yoshimori T
  • Journal Title: Cell Host Microbe Volume: 11(5) Pages: 457-468
  • Peer Reviewed
• [Journal Article] Vacuolating Cytotoxin and Variants in Atg16L1 That Disrupt Autophagy Promote Helicobacter pylori Infection in Humans (2012)
  • Author(s): Raju D, Hussey S, Ang M, Terebiznik MR, Sibony M, Galindo-Mata E, Gupta V, Blanke SR, Delgado A, Romero-Gallo J, Ramjeet MS, Mascarenhas H, Peek RM, Correa P, Streutker C, Hold G, Kunstmann E, Yoshimori T, Silverberg MS, Girardin SE, Philpott DJ, El Omar
  • Journal Title: Gastroenterology Volume: 142(5) Pages: 1160-1171
  • Peer Reviewed
• [Journal Article] Autophagy guards against Cisplatin-induced acute kidney injury (2012)
  • Author(s): Takahashi A, Kimura T, Takabatake Y, Namba T, Kaimori J, Kitamura H*, Matsui I, Niimura F, Matsusaka T, Fujita N, Yoshimori T, Isaka Y*, Rakugi H
  • Journal Title: Am J Pathol Volume: 180(2) Pages: 517-525
  • Peer Reviewed
• [Journal Article] Vacuolating Cytotoxin and Variants in Atg16L1 That Disrupt Autophagy Promote Helicobacter pylori Infection in Humans (2012)
  • Author(s): Raju D, Hussey S, Ang M, Terebiznik MR, Sibony M, Galindo-Mata E, Gupta V, Blanke SR, Delgado A, Romero-Gallo J, Ramjeet MS, Mascarenhas H, Peek RM, Correa P, Streutker C, Hold G, Kunstmann E, Yoshimori T, Silverberg MS, Girardin SE, Philpott DJ, El Omar E, Jones NL
  • Journal Title: Gastroenterology. Volume: 142 Issue: 5 Pages: 1160-71
  • DOI: 10.1053/j.gastro.2012.01.043
  • Peer Reviewed
• [Journal Article] Autophagy guards against Cisplatin-induced acute kidney injury. (2012)
  • Author(s): Takahashi A, Kimura T, Takabatake Y, Namba T, Kaimori J, Kitamura H*, Matsui I, Niimura F, Matsusaka T, Fujita N, Yoshimori T, Isaka Y*, Rakugi H.
  • Journal Title: Am J Pathol. Volume: 180 Pages: 517-25
  • Peer Reviewed
• [Journal Article] Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an Atg12-5-16L2 complex (2011)
  • Author(s): Ishibashi K, Fujita N, Kanno E, Omori H, Yoshimori T, Itoh T, Fukuda M*
  • Journal Title: Autophagy Volume: 7(12) Pages: 1500-1513
  • Peer Reviewed
• [Journal Article] Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus (2011)
  • Author(s): Taguwa S, Kambara H, Fujita N, Noda T, Yoshimori T, Koike K, Moriishi K, Matsuura Y*
  • Journal Title: J Virol Volume: 85(24) Pages: 13185-13194
  • Peer Reviewed
• [Journal Article] A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery (2011)
  • Author(s): Katayama H, Kogure T, Mizushima N, Yoshimori T, Miyawaki A*
  • Journal Title: Chem Biol Volume: 18(8) Pages: 1042-1052
  • Peer Reviewed
• [Journal Article] The LC3 recruitment mechanism is separate from Atg9L1-dependent membrane formation in the autophagic response against Salmonella (2011)
  • Author(s): Kageyama S, Omori H, Saitoh T, Sone T, Guan JL, Akira S, Imamoto F, Noda T, Yoshimori T*
  • Journal Title: Mol Biol Cell Volume: 22 Pages: 2290-2300
  • Peer Reviewed
• [Journal Article] Proteome of ubiquitin/MVB pathway: possible involvement of iron-induced ubiquitylation of transferrin receptor in lysosomal degradation (2011)
  • Author(s): Tachiyama R, Ishikawa D, Matsumoto M, Nakayama KI, Yoshimori T, Yokota S, Himeno M, Tanaka Y, Fujita H*
  • Journal Title: Genes Cells Volume: 16 Pages: 448-466
  • Peer Reviewed
• [Journal Article] Inhibition of autophagy potentiates the antitumor effect of the multikinase inhibitor sorafenib in hepatocellular carcinoma (2011)
  • Author(s): Shimizu S, Takehara T, Hikita H, Kodama T, Tsunematsu H, Miyagi T, Hosui A, Ishida H, Tatsumi T, Kanto T, Hiramatsu N, Fujita N, Yoshimori T, Hayashi N
  • Journal Title: Int.J.Cancer Volume: 131 Issue: 3 Pages: 548-57
  • DOI: 10.1002/ijc.26374
  • Peer Reviewed
• [Journal Article] The LC3 recruitment mechanism is separate from Atg9L1-dependent membrane formation in the autophagic response against Salmonella. (2011)
  • Author(s): Kageyama S, Omori H, Saitoh T, Sone T, Guan JL, Akira S, Imamoto F, Noda T*, Yoshimori T*.
  • Journal Title: Mol Biol Cell. Volume: 22 Pages: 2290-300
  • Peer Reviewed
• [Journal Article] A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. (2011)
  • Author(s): Katayama H, Kogure T, Mizushima N, Yoshimori T, Miyawaki A*.
  • Journal Title: Chem Biol. Volume: 18 Pages: 1042-52
  • Peer Reviewed
• [Journal Article] Proteome of ubiquitin/MVB pathway : possible involvement of iron-induced ubiquitylation of transferrin receptor in lysosomal degradation (2011)
  • Author(s): Tachiyama R, Ishikawa D, Matsumoto M, Nakayama KI, Yoshimori T, Yokota S, Himeno M, Tanaka Y, Fujita H
  • Journal Title: Genes Cells Volume: 16 Issue: 4 Pages: 448-66
  • DOI: 10.1111/j.1365-2443.2011.01499.x
  • Peer Reviewed
• [Journal Article] Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. (2011)
  • Author(s): Taguwa S, Kambara H, Fujita N, Noda T, Yoshimori T, Koike K, Moriishi K, Matsuura Y*.
  • Journal Title: J Virol. Volume: 85 Pages: 13185-94
  • Peer Reviewed
• [Journal Article] Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an Atg12-5-16L2 complex. (2011)
  • Author(s): Ishibashi K, Fujita N, Kanno E, Omori H, Yoshimori T, Itoh T, Fukuda M*.
  • Journal Title: Autophagy Volume: 7
  • Peer Reviewed
• [Presentation] 50 Years of Autophagy: towards Understanding The Intracellular Self-Degradation System Sustaining Life (2013)
  • Author(s): Tamotsu Yoshimori
  • Organizer: 2013 The 28th Joint Annual Conference of Biomedical Sciences
  • Place of Presentation: National Defense Medical Center(Taipei,Taiwan)
  • Invited
• [Presentation] Mechanistic Insights into Autophagy against Bacteria Invading Host Cells (2012)
  • Author(s): Tamotsu Yoshimori
  • Organizer: Cambridge Immunology Forum 2012
  • Place of Presentation: Cambridge, UK
  • Year and Date: 2012-09-20
• [Presentation] Insights into Membrane Dynamics in Mammalian Autophagy (2012)
  • Author(s): 吉森 保
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・ﾏﾘﾝﾒｯｾ福岡（福岡）
  • Invited
• [Presentation] オートファジー：発見から半世紀を経て (2012)
  • Author(s): 吉森 保
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際会議場（神戸）
  • Invited
• [Presentation] 哺乳類オートファジーの膜動態と疾患との関わり (2012)
  • Author(s): 吉森 保
  • Organizer: 第28回京都賞記念ワークショップ基礎科学部門
  • Place of Presentation: 国立京都国際会館（京都）
  • Invited
• [Presentation] Autophagy : Molecular Machinery and Role in Diseases (2012)
  • Author(s): Tamotsu Yoshimori
  • Organizer: The 12th International Symposium on Dendritic Cells
  • Place of Presentation: Convention Center EXCO(Daegu,Korea)
  • Invited
• [Presentation] Mechanistic Insights into Autophagy against Bacteria Invading Host Cells (2012)
  • Author(s): Tamotsu Yoshimori
  • Organizer: Cambridge Immunology Forum 2012
  • Place of Presentation: Peterhouse in Cambridge University(Cambridge,UK)
  • Invited
• [Presentation] New insights into selective autophagy (2012)
  • Author(s): Tamotsu Yoshimori
  • Organizer: Beatson International Cancer Conference
  • Place of Presentation: Glasgow University(Glasgow, Scotland)
  • Invited
• [Presentation] 生体防御システムとしてのオートファジー (2012)
  • Author(s): 吉森 保
  • Organizer: 第17回東京肝臓シンポジウム
  • Place of Presentation: 経団連会館（東京）
  • Invited
• [Presentation] 15 Years of Autophagy: Towards Understanding The Intracellular Self-Degradation System Sustaining Life (2012)
  • Author(s): Tamotsu Yoshimori
  • Organizer: Frontiers of Biological Sciences Seminar Series in Tsinghua University
  • Place of Presentation: 精華大学（北京、中国）
  • Invited
• [Presentation] Mechanistic insights into membrane dynamics in autophagy (2012)
  • Author(s): Tamotsu Yoshimori
  • Organizer: ASBMB 2012 Annual Meeting
  • Place of Presentation: San Diego Convention Center(San Diego ,USA)
  • Invited
• [Presentation] Autophagy : molecular machinery and role in diseases (2012)
  • Author(s): Tamotsu Yoshimori
  • Organizer: BSCB- BSDB- JSDB Joint Spring Meeting
  • Place of Presentation: University of Warwick(Coventry, UK)
  • Invited
• [Presentation] Autophagy: Molecular Machinery, Membrane Dynamics, and Role in Diseases (2012)
  • Author(s): Tamotsu Yoshimori
  • Organizer: ASI シンポジウム’Frontier in Cell Biology Research’(招待講演)
  • Place of Presentation: The Chinese University of Hong Kong, China
• [Presentation] Insights into Membrane Dynamics in Selective and Non-Selective Autophagy (2012)
  • Author(s): 吉森 保
  • Organizer: 2012 Senri Life Science Int. Symposium on ‘Cutting-edge of Autophagy Study’(招待講演)
  • Place of Presentation: 千里ライフサイエンスセンタービル、大阪
• [Presentation] オートファジー：生命を護る細胞内大規模分解システム (2012)
  • Author(s): 吉森 保
  • Organizer: 第24回気道病態研究会(招待講演)
  • Place of Presentation: 東京ステーションコンファレンス、東京
• [Presentation] Insights into Membrane Dynamics in Autophagy (2011)
  • Author(s): Tamotsu Yoshimori
  • Organizer: EMBO Conference on Autophagy in Health and Disease in Tel Aviv
  • Place of Presentation: Israel
  • Year and Date: 2011-10-31
• [Presentation] Autophagy, A Guardian of Cells against Infection (2011)
  • Author(s): Tamotsu Yoshimori
  • Organizer: The 19th Federation Meeting of Korean Basic Medical Scientists(招待講演)
  • Place of Presentation: Grand Hilton Seoul, Convention Center, Korea
• [Presentation] Mechanistic Insight into Membrane Dynamics in Autophagy (2011)
  • Author(s): 吉森 保
  • Organizer: 第30回内藤コンファレンス(招待講演)
  • Place of Presentation: シャトレーゼガトーキングダムサッポロ、北海道
• [Presentation] Autophagosome Formation: between the ER and Mitochondria (2011)
  • Author(s): Tamotsu Yoshimori
  • Organizer: Gordon Research Conference ‘Molecular Membrane Biology’(招待講演)
  • Place of Presentation: Proctor Academy,Andover, NH, U.S.A.
• [Presentation] Where Does Autophagosome Come from?: Membrane Biogenesis in Autophagy (2011)
  • Author(s): 吉森 保
  • Organizer: 第2回日中オートファジーシンポジウム(招待講演)
  • Place of Presentation: 湘南国際村センター、神奈川
• [Presentation] 非選択的および選択的オートファジーにおける膜創成 (2011)
  • Author(s): 吉森 保
  • Organizer: 第１回都医学研シンポジウム(招待講演)
  • Place of Presentation: 津田ホール、東京
• [Presentation] Insights into Membrane Dynamics in Autophagy (2011)
  • Author(s): Tamotsu Yoshimori
  • Organizer: EMBO Conference on Autophagy in Health and Disease(招待講演)
  • Place of Presentation: Ma’ale Hachamisha Hotel,,Israel
• [Presentation] Autophagy: New Insights into Its Membrane Dynamics (2011)
  • Author(s): Tamotsu Yoshimori
  • Organizer: The 2nd Int. Symposium on Ubiquitin and Ubiquitin-Like Modifications(招待講演)
  • Place of Presentation: Sinica ｱｶﾃﾞﾐｱ生物医科学研究所、台北、台湾
• [Presentation] Autophagy: Molecular Machinery and Role in Diseases (2011)
  • Author(s): 吉森 保
  • Organizer: 第27回熊本医学・生物科学シンポジウム(招待講演)
  • Place of Presentation: 熊本大学山崎記念館、熊本
• [Presentation] New Insights into Selective Autophagy (2011)
  • Author(s): Tamotsu Yoshimori
  • Organizer: Zing Conference on Autophagy 2011(招待講演)
  • Place of Presentation: Occidental Grand Xcaret,Mexico
• [Book] Q&A / 哺乳類におけるオートファジー関連因子オートファジー 生命をささえる細胞の自己分解システム水島昇・吉森保 編 (2012)
  • Author(s): 吉森 保
  • Publisher: 化学同人
• [Book] オートファジー 生命をささえる細胞の自己分解システム (2012)
  • Author(s): 吉森 保
  • Publisher: 化学同人
• [Book] Cell Imaging Techniques: Methods and Protocols Second Edition (2012)
  • Author(s): Keisuke T, Mitsuko H-N, Takeshi N, Akitsugu Y,Yoshimori T.
  • Publisher: Humana Press
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/jp/achievement/010/
• [Remarks] 研究室ＨＰ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/jp/achievement/010/