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The influence of transcript localization on post-translational modification

Research Project

Project/Area Number 23657137
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionRikkyo University (2012)
Keio University (2011)

Principal Investigator

YAMAMOTO Miki  立教大学, 理学部, ポストドクトラルフェロー (40301783)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords細胞構造 / 機能 / GPIアンカー修飾 / 翻訳後修飾 / GPI / mRNA
Research Abstract

Post-translational modification of protein is important in promoting proper function of protein. In this study, we investigated whether post-translational modification of protein is regulated by mRNA localization or not. Dally-like protein (Dlp) in Drosophila has GPI-anchor which is a type of post-translational modification. And dlp mRNA shows restricted distribution near nucleus in wing disc cells. We changed the localization of dlp mRNA by replacing 3’-noncoding region of dlp with that of wingless which contains apical localization signal. The products translated from mislocalized mRNA did not show proper function and were accumulated in apical region of cells, suggesting that mRNA localization is involved in protein function and probably post-translationalmodification.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (23 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (16 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Cisterna-specific localization of glycosylation-related proteins to the Golgi apparatus.2012

    • Author(s)
      Yamamoto-Hino,M., Abe,M., Shibano,T., Setoguchi,Y., Awano,W., Ueda.R., Okano,H.and Goto,S. Cell Struct.
    • Journal Title

      Funct

      Volume: 37 Pages: 55-63

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of proteasome components required for apical localization of Chaoptin using functional genomics2012

    • Author(s)
      Yano,H., Yamamoto-Hino,M., Awano,W., Aoki- Kinoshita,K.F., Tsuda-Sakurai,K., Okano,H. and Goto,S.
    • Journal Title

      J. Neurogenet.

      Volume: 26 Pages: 53-63

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cisterna-specific Localization of Glycosylation-related Proteins to the Golgi Apparatus2012

    • Author(s)
      Yamamoto-Hino, M., Abe, M., Shibano, T., Setoguchi, Y., Awano, W., Ueda. R., Okano, H., Goto, S.
    • Journal Title

      Cell Structure and Function

      Volume: 37 Issue: 1 Pages: 55-63

    • DOI

      10.1247/csf.11037

    • NAID

      130004137577

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of proteasome components required for apical localization of Chaoptin using functional genomics2012

    • Author(s)
      Yano, H., Yamamoto-Hino, M., Awano, W., Aoki-Kinoshita, K. F., Tsuda-Sakurai, K., Okano, H. and Goto, S.
    • Journal Title

      J. Neurogenet

      Volume: vol.26 Issue: 1 Pages: 53-63

    • DOI

      10.3109/01677063.2012.661497

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ショウジョウバエゴルジ体に局在する Golgi complex-relatedglycoprotein1の同定と自然免疫における機能2013

    • Author(s)
      山本(日野)美紀
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋ウインク愛知
    • Year and Date
      2013-06-19
    • Related Report
      2012 Final Research Report
  • [Presentation] Golgi complex-related glycoprotein 1 and its function in innate immunity2013

    • Author(s)
      山本(日野)美紀
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋 愛知県産業労働センター
    • Related Report
      2012 Annual Research Report
  • [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity2012

    • Author(s)
      山本(日野)美紀
    • Organizer
      International Endotoxin and Innate Immunity Society Meeting
    • Place of Presentation
      東京National Center ofSciences Building
    • Year and Date
      2012-10-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity2012

    • Author(s)
      山本(日野)美紀
    • Organizer
      第10回日本ショウジョウバエ研究会
    • Place of Presentation
      東京慈恵医大
    • Year and Date
      2012-10-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity2012

    • Author(s)
      山本(日野)美紀
    • Organizer
      第45回日本発生生物学会、第64回日本細胞生物学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-05-31
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity2012

    • Author(s)
      山本(日野)美紀
    • Organizer
      第45回日本発生生物学会、第64回日本細胞生物学会
    • Place of Presentation
      神戸 国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity2012

    • Author(s)
      山本(日野)美紀
    • Organizer
      第10回日本ショウジョウバエ研究会
    • Place of Presentation
      東京 慈恵医大
    • Related Report
      2012 Annual Research Report
  • [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity2012

    • Author(s)
      山本(日野)美紀
    • Organizer
      International Endotoxin and Innate Immunity Society Meeting
    • Place of Presentation
      東京 National Center of Sciences Building
    • Related Report
      2012 Annual Research Report
  • [Presentation] Novel Roles ofGlycosylation in Drosophila InnateImmunity2011

    • Author(s)
      山本(日野)美紀
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      神奈川横浜パシフィコ
    • Year and Date
      2011-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析2011

    • Author(s)
      山本(日野)美紀
    • Organizer
      第40回日本免疫学会学術集会
    • Place of Presentation
      千葉幕張メッセ
    • Year and Date
      2011-11-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析2011

    • Author(s)
      山本(日野)美紀
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都京都国際会議場
    • Year and Date
      2011-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity2011

    • Author(s)
      山本(日野)美紀
    • Organizer
      1 st Asia-Pacific Drosophila Research Conference
    • Place of Presentation
      Chientan Youth Activity Center台湾
    • Year and Date
      2011-05-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity2011

    • Author(s)
      Miki Yamamoto-Hino, Takako Shibano, Wakae Awano, Hideyuki Okano, Satoshi Goto
    • Organizer
      1 st Asia-Pacific Drosophila Research Conference
    • Place of Presentation
      Chientan Youth Activity Center, Taipei
    • Related Report
      2011 Research-status Report
  • [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析2011

    • Author(s)
      山本(日野)美紀、粟野若枝、芝野孝子、岡野栄之、後藤聡
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都 京都国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析2011

    • Author(s)
      山本(日野)美紀、岡野栄之、後藤聡
    • Organizer
      第40回日本免疫学会学術集会
    • Place of Presentation
      千葉 幕張メッセ
    • Related Report
      2011 Research-status Report
  • [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity2011

    • Author(s)
      山本(日野)美紀、芝野孝子、粟野若枝、村岡正敏、岡野栄之、後藤聡
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      神奈川 横浜パシフィコ
    • Related Report
      2011 Research-status Report
  • [Book] Structure, Function and Formation of Glycans in Drosophila. In "Glycans: Biochemistry, Characterization and Applications.2012

    • Author(s)
      Yamamoto-Hino,M., Okano,H., Kanie,O. and Goto,S.
    • Publisher
      Nova Science publishers, Inc., NY
    • Related Report
      2012 Final Research Report
  • [Book] Structure, Function and Formation of Glycans in Drosophila. In "Glycans: Biochemistry, Characterization and Applications."2012

    • Author(s)
      Yamamoto-Hino,M., Okano,H., Kanie,O. and Goto,S.
    • Total Pages
      24
    • Publisher
      Nova Science publishers, Inc., NY
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://goto-lab.net/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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