The influence of transcript localization on post-translational modification
| Project/Area Number |
23657137
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Rikkyo University (2012)
Keio University (2011) |
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Principal Investigator |
YAMAMOTO Miki 立教大学, 理学部, ポストドクトラルフェロー (40301783)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 細胞構造 / 機能 / GPIアンカー修飾 / 翻訳後修飾 / GPI / mRNA |
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| Research Abstract |
Post-translational modification of protein is important in promoting proper function of protein. In this study, we investigated whether post-translational modification of protein is regulated by mRNA localization or not. Dally-like protein (Dlp) in Drosophila has GPI-anchor which is a type of post-translational modification. And dlp mRNA shows restricted distribution near nucleus in wing disc cells. We changed the localization of dlp mRNA by replacing 3’-noncoding region of dlp with that of wingless which contains apical localization signal. The products translated from mislocalized mRNA did not show proper function and were accumulated in apical region of cells, suggesting that mRNA localization is involved in protein function and probably post-translationalmodification.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Cisterna-specific localization of glycosylation-related proteins to the Golgi apparatus.2012
Author(s)
Yamamoto-Hino,M., Abe,M., Shibano,T., Setoguchi,Y., Awano,W., Ueda.R., Okano,H.and Goto,S. Cell Struct.
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Journal Title
Funct
Volume: 37
Pages: 55-63
Related Report
Peer Reviewed
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[Journal Article] Identification of proteasome components required for apical localization of Chaoptin using functional genomics2012
Author(s)
Yano,H., Yamamoto-Hino,M., Awano,W., Aoki- Kinoshita,K.F., Tsuda-Sakurai,K., Okano,H. and Goto,S.
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Journal Title
J. Neurogenet.
Volume: 26
Pages: 53-63
Related Report
Peer Reviewed
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[Journal Article] Cisterna-specific Localization of Glycosylation-related Proteins to the Golgi Apparatus2012
Author(s)
Yamamoto-Hino, M., Abe, M., Shibano, T., Setoguchi, Y., Awano, W., Ueda. R., Okano, H., Goto, S.
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Journal Title
Cell Struct. Funct.
Volume: 37
Issue: 1
Pages: 55-63
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed
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[Journal Article] 2. Identification of proteasome components required for apical localization of Chaoptin using functional genomics.2012
Author(s)
Yano, H., Yamamoto-Hino, M., Awano, W., Aoki-Kinoshita, K. F., Tsuda-Sakurai, K., Okano, H. and Goto, S.
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Journal Title
J. Neurogenet.
Volume: vol.26
Pages: 53-63
DOI
Related Report
Peer Reviewed
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[Book] Structure, Function and Formation of Glycans in Drosophila. In "Glycans: Biochemistry, Characterization and Applications.2012
Author(s)
Yamamoto-Hino,M., Okano,H., Kanie,O. and Goto,S.
Publisher
Nova Science publishers, Inc., NY
Related Report
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[Book] Structure, Function and Formation of Glycans in Drosophila. In "Glycans: Biochemistry, Characterization and Applications."2012
Author(s)
Yamamoto-Hino,M., Okano,H., Kanie,O. and Goto,S.
Total Pages
24
Publisher
Nova Science publishers, Inc., NY
Related Report
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