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Ability to form meiotic spindle is dependent on GV material

Research Project

Project/Area Number 23657143
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionOchanomizu University

Principal Investigator

CHIBA Kazuyoshi  お茶の水女子大学, 大学院・人間文化創成科学研究科, 教授 (70222130)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords受精 / 減数分裂 / 卵母細胞 / ヒトデ / 星状体 / 多精 / 卵核胞 / 紡錘体 / 染色体 / 精子 / 卵 / 星状j体
Research Abstract

Before spawning, maternal asters existing near GV are affected by soluble components of GV other than chromosomes during GVBD, and acquire the ability to form functional spindles. Then, spawned oocytes are fertilized at MI. Although centrosomes of sperm form sperm asters, they wait to participate in cell cycle events until oocyte meiosis is completed: They cannot form functional spindles during meiotic division of oocytes, because GV components have been dispersed throughout the cytoplasm. When oocytes are isolated from the ovaries and artificially inseminated before GVBD or MI, polyspermy occurs and sperm asters at the animal pole are affected by GV components during GVBD and form abnormal multi-polar spindles interacting with maternal chromosomes during MI and MII. Thus, both spawning and fertilization at MI are important for maturing oocytes to be protected from formation of abnormal multi-polar spindles by paternal centrosomes.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (9 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (4 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Sperm from sneaker male squids exhibit chemotactic swarming to CO_22013

    • Author(s)
      Hirohashi N, Alvarez L, Shiba K, Fujiwara E, Iwata Y, Mohri T, Inaba K, Chiba K, Ochi H, Supuran CT, Kotzur N, Kakiuchi Y, Kaupp UB, Baba SA
    • Journal Title

      Current Biology

      Volume: 23 Issue: 9 Pages: 775-781

    • DOI

      10.1016/j.cub.2013.03.040

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Symmetrical division of mouse oocytes during meiotic maturation can lead to the development of twin embryos that amalgamate to form a chimeric hermaphrodite2012

    • Author(s)
      Otsuki, J., Nagai, Y., Lopata, A., Chiba, K., Yasmin, L., and Sankai, T
    • Journal Title

      Hum. Reprod

      Volume: 27巻 Issue: 2 Pages: 80-387

    • DOI

      10.1093/humrep/der408

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of the acquisition offertilization competence and polyspermy blocks during meiotic maturation2011

    • Author(s)
      Chiba, K
    • Journal Title

      Mol. Reprod. Dev

      Volume: 808-813 Issue: 10-11 Pages: 808-813

    • DOI

      10.1002/mrd.21378

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Arrest at metaphase of meiosis I in starfish oocytes in the ovary is maintained by high CO2 and low O2 concentrations in extracellular fluid Zool

    • Author(s)
      Moriwaki K, Nakagawa T, Nakaya F, Hirohashi N, Chiba K
    • Journal Title

      Sci

      Volume: (印刷中)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Meiotic arrest and fertilization of starfish oocytes2013

    • Author(s)
      千葉和義
    • Organizer
      Oocyte maturation and fertilization meeting: Lessons from canonical and emerging models
    • Place of Presentation
      in Banyuls-sur-Mer, France
    • Year and Date
      2013-06-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 卵減数分裂に干渉しない精子星状体2012

    • Author(s)
      長谷川翠、千葉和義
    • Organizer
      日本動物学会第83大会
    • Place of Presentation
      大阪大学
    • Year and Date
      2012-09-13
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] ヒトデMI停止機構2012

    • Author(s)
      千葉和義
    • Organizer
      日本動物学会第83大会
    • Place of Presentation
      大阪大学
    • Year and Date
      2012-09-12
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒトデ卵MI停止機構2012

    • Author(s)
      千葉和義
    • Organizer
      日本動物学会第83大会
    • Place of Presentation
      大阪大学
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 反応方法および反応装置2012

    • Inventor(s)
      千葉和義
    • Industrial Property Rights Holder
      千葉和義
    • Industrial Property Number
      2012-016684
    • Filing Date
      2012-07-27
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Overseas

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Published: 2011-08-05   Modified: 2019-07-29  

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