| Project/Area Number |
23657144
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Nagoya University |
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Principal Investigator |
TAKAGI Shin 名古屋大学, 理学(系)研究科(研究院), 准教授 (90171420)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 分化運命 / C. elegans / 分化運命決定 / 転写因子 / 線虫C. elegans |
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| Research Abstract |
Recent studies show that ectopic expression of specific combinations of transcription factors can change identity of apparently differentiated cells. To reveal the underlying mechanisms of reprogramming we here take the worm C. elegans, in which the developmental fate of each cell appears to be determined strictly. In larval worms that have completed neurogenesis, forced expression of a mixture of transcription factors induced production of cells that ectopically express pan-neuronal markers. Many of them assumed neuron-like morphology and expressed a marker for differentiated sensory cells, suggesting they underwent complete reprogramming, while some assumed non-neuron-like morphology and co-expressed a maker of a non-neuronal cell type. Thus, we have successfully established an in vivo model for deciphering the cellular contexts necessary for cell reprogramming.
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