Establishment of a novel approach to select the best iPSCs without cell cloning

• Project/Area Number: 23657147
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: SAITO Megumu 京都大学, iPS 細胞研究所, 准教授 (90535486)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 幹細胞 / iPS細胞 / クローニング / 血球分化

Research Abstract

I set out to develop a novel technology that enables to derivate iPS cells which can differentiate into desired differentiated cell types without cell cloning during reprograming. I constructed gene vectors that is required for this system, and developed a novel hematopoietic cell differentiation system which can efficientlyderivate immature hematopoietic precursors and mature hematopoietic cells. I introduced constructed vectors into iPS cells and recovering clones which have modified allele in AAVS1 site by homologous recombination.

Research Products

• [Journal Article] Robust and highly-efficient differentiation of functional monocytic cells from human pluripotent stem cells under serum- and feeder cell- free conditions. (2013)
  • Author(s): Yanagimachi MD, Niwa A, Tanaka T, Ozaki F, Nishimoto S, Murata Y, Yasumi T, Ito J, Tomida S, Oshima K, Asaka I, Goto H, Heike T, Nakahata T, Saito MK
  • Journal Title: PLoS ONE Volume: 8(4) Issue: 4 Pages: e59243-e59243
  • DOI: 10.1371/journal.pone.0059243
  • NAID: 120005244458
  • Peer Reviewed
• [Journal Article] Disease associated iPS cell lines representing hematological and immunological disorders. (2012)
  • Author(s): Saito MK*, Niwa A.
  • Journal Title: Inflammation and regeneration. Volume: 32(4) Pages: 171-7
  • URL: http://www.jsir.gr.jp/journal/Vol32No4/contents.html
  • Peer Reviewed
• [Journal Article] Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery (2012)
  • Author(s): Tanaka T., Takahashi K., Yamane M., Tomida S., Nakamura S., Oshima K., Niwa A., Nishikomori R., Kambe N., Hara H., Mitsuyama M., Morone N., Heuse J.E., Yamamoto T., Watanabe A., Sato-Otsubo A., Ozawa S., Asaka I., Heike T., Yamanaka S., Nak
  • Journal Title: Blood Volume: 120(6) Issue: 6 Pages: 1299-308
  • DOI: 10.1182/blood-2012-03-417881
  • Peer Reviewed
• [Journal Article] Frequent somatic mosaicism of NEMO in T cells of patients with X-linked anhidrotic ectodermal dysplasia with immune- deficiency (2012)
  • Author(s): Kawai T, Imai C et al
  • Journal Title: Blood Volume: 119 Issue: 23 Pages: 5458-66
  • DOI: 10.1182/blood-2011-05-354167
  • Peer Reviewed
• [Journal Article] Multiple reversions of an IL2RG mutation restore combined immunodeficiency patient. (2012)
  • Author(s): Kawai T., Saito M., Nishikomori R., Yasumi T., Izawa K., Murakami T., Okamoto N., Mori Y., Nakagawa N., Imai K., Nonoyama S., Wada T., Yatie A., Oomori K., Nakahata T., Heike T.
  • Journal Title: J. Clin. Immunol Volume: 32(4) Issue: 4 Pages: 690-7
  • DOI: 10.1007/s10875-012-9684-1
  • Peer Reviewed
• [Journal Article] Detection of Base Substitution-Type Somatic Mosaicism of the NLRP3 Gene with> 99. 9% Statistical Confidence by Massively Parallel Sequencing (2012)
  • Author(s): Izawa, K., A. Hijikata, N. Tanaka, T. Kawai, M. K. Saito, R. Goldbach-Mansky, I. Aksentijevich, T. Yasumi, T. Nakahata, T. Heike, R. Nishikomori, and O. Ohara
  • Journal Title: DNA Res Volume: 19 Issue: 2 Pages: 143-52
  • DOI: 10.1093/dnares/dsr047
  • Peer Reviewed
• [Journal Article] Disease-associated iPS cell lines representing hematological and immunological disorders. (2012)
  • Author(s): Saito MK*, Niwa A.
  • Journal Title: Inflammation and regeneration Volume: 32 Pages: 171-177
  • Peer Reviewed
• [Journal Article] Identification of Hepatic Niche Harboring Human Acute Lymphoblastic Leukemic Cells via the SDF-1/CXCR4 Axis (2011)
  • Author(s): Itaru Kato
  • Journal Title: PLos one Volume: 6 Issue: 11 Pages: e27042-e27042
  • DOI: 10.1371/journal.pone.0027042
  • Peer Reviewed
• [Journal Article] A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors (2011)
  • Author(s): Niwa A, Heike T, Umeda K, et al.
  • Journal Title: PloS One Volume: 6 Issue: 7 Pages: e22261-e22261
  • DOI: 10.1371/journal.pone.0022261
  • NAID: 120003324603
  • Peer Reviewed
• [Presentation] iPS 細胞研究の現状と展望 (2013)
  • Author(s): 齋藤潤
  • Organizer: GMCC 講習会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-09
• [Presentation] 疾患 iPS 細胞を用いた小児難治性疾患の病態解析 (2012)
  • Author(s): 齋藤潤
  • Organizer: 日本小児科学会京都地方会
  • Place of Presentation: 京都
  • Year and Date: 2012-12-15
• [Presentation] 疾患特異的 iPS 細胞を用いた血液・免疫疾患の病態解析 (2012)
  • Author(s): 齋藤潤
  • Organizer: 久留米大学膠原病内科セミナー
  • Place of Presentation: 久留米
  • Year and Date: 2012-11-08
• [Presentation] 疾患 iPS細胞を用いた血液疾患の病態解析 (2012)
  • Author(s): 齋藤潤
  • Organizer: CiRA mini symposium 2012
  • Place of Presentation: 京都
  • Year and Date: 2012-10-10
• [Presentation] ヒト iPS細胞からの造血分化と疾患解析への応用 (2012)
  • Author(s): 齋藤潤
  • Organizer: 第22回日本産婦人科・新生児血液学会
  • Place of Presentation: 津
  • Year and Date: 2012-06-29
• [Presentation] 疾患iPS細胞研究の最前線 (2012)
  • Author(s): 齋藤潤
  • Organizer: 第124回遠江医学会
  • Place of Presentation: 浜松
  • Year and Date: 2012-06-17
• [Presentation] ヒト疾患特異的iPS細胞を用いた難治性免疫・血液疾患の病態解析 (2012)
  • Author(s): 齋藤潤
  • Organizer: 平成24年度第1回難治研大学院セミナー
  • Place of Presentation: 川崎
  • Year and Date: 2012-05-17
• [Presentation] 疾患特異的iPS細胞を用いた 免疫疾患の解析について (2011)
  • Author(s): 齋藤潤
  • Organizer: 第39回日本臨床免疫学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-15
• [Presentation] 自己炎症性疾患 (2011)
  • Author(s): 齋藤潤
  • Organizer: 第114回 日本小児科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-08-12
• [Presentation] 自己炎症性症候群とiPS細胞 (2011)
  • Author(s): 齋藤潤
  • Organizer: 第55回日本リウマチ学会
  • Place of Presentation: 神戸
  • Year and Date: 2011-07-18
• [Presentation] Disease modeling of Chronic Infantile Neurologic Cutaneous and Articular syndrome with somatic mosaicism via patient-specific induced pluripotent stem cells. (2011)
  • Organizer: the 10th World Congress on Inflammation in Paris
  • Place of Presentation: Paris, France
  • Year and Date: 2011-06-25
• [Presentation] Disease modeling of human immunological disorders with iPS cells (2011)
  • Author(s): 齋藤潤
  • Organizer: JSIR2011/APFIR2011(第32回日本炎症再生学会総会)
  • Place of Presentation: 東京
  • Year and Date: 2011-06-02
• [Presentation] ヒト疾患特異的iPS細胞を用いた 難治性免疫・血液疾患の病態解析
  • Author(s): 齋藤潤
  • Organizer: 平成24年度第1回難治研大学院セミナー
  • Place of Presentation: 川崎市
  • Invited
• [Presentation] 疾患iPS細胞研究の最前線
  • Author(s): 齋藤潤
  • Organizer: 第124回遠江医学会
  • Place of Presentation: 浜松市
  • Invited
• [Presentation] ヒトiPS細胞からの造血分化と 疾患解析への応用
  • Author(s): 齋藤潤
  • Organizer: 第22回日本産婦人科・新生児血液学会
  • Place of Presentation: 津市
  • Invited
• [Presentation] 疾患iPS細胞を用いた血液疾患の病態解析
  • Author(s): 齋藤潤
  • Organizer: CiRA mini symposium 2012
  • Place of Presentation: 京都市
  • Invited
• [Presentation] 疾患iPS細胞を用いた小児難治性疾患の病態解析
  • Author(s): 齋藤潤
  • Organizer: 日本小児科学会京都地方会
  • Place of Presentation: 京都市
  • Invited
• [Book] 【疾患特異的iPS細胞】 疾患特異的iPS細胞を用いた難治性血液・免疫疾患への取り組み BIO clinica 28(3) (2013)
  • Author(s): 齋藤潤
• [Book] 疾患特異的iPS細胞再生医療12(1) (2013)
  • Author(s): 齋藤潤、中畑龍俊
• [Book] ヒトiPS細胞からの造血分化と疾患解析への応用日本産科新生児血液学会雑誌 22 (2) (2013)
  • Author(s): 齋藤潤
• [Book] 【炎症シグナルの指揮者インフラマソーム】 インフラマソームと自己炎症性症候群 実験医学 30(11) (2012)
  • Author(s): 齋藤潤
• [Book] 【自己炎症疾患の新しい知見】 CAPS:クライオパイリン関連周期熱症候群 日本臨床免疫学会会誌 34(5) (2011)
  • Author(s): 齋藤潤
• [Book] 【iPS 細胞の臨床応用の展望】 疾患特異的iPS細胞の臨床応用 BIO clinica 26 (9) (2011)
  • Author(s): 齋藤潤
• [Book] インフラマソームとその関連疾患の最近の知見 日本臨床免疫学会会誌 34 (1) (2011)
  • Author(s): 斎藤潤
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/nakahata/
• [Patent(Industrial Property Rights)] Blood analysis apparatus and blood analysis method (2012)
  • Inventor(s): Matsui Eriko; Dowaki Suguru; Tatsuta Hirokazu; Kunihiro Takeshi; Niwa Akira; Saito Megumu; Nakahata Tatsutoshi
  • Industrial Property Rights Holder: Matsui Eriko; Dowaki Suguru; Tatsuta Hirokazu; Kunihiro Takeshi; Niwa Akira; Saito Megumu; Nakahata Tatsutoshi
  • Filing Date: 2012-07-30
  • Overseas