Establishment of a novel approach to select the best iPSCs without cell cloning
| Project/Area Number |
23657147
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
SAITO Megumu 京都大学, iPS 細胞研究所, 准教授 (90535486)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 幹細胞 / iPS細胞 / クローニング / 血球分化 |
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| Research Abstract |
I set out to develop a novel technology that enables to derivate iPS cells which can differentiate into desired differentiated cell types without cell cloning during reprograming. I constructed gene vectors that is required for this system, and developed a novel hematopoietic cell differentiation system which can efficientlyderivate immature hematopoietic precursors and mature hematopoietic cells. I introduced constructed vectors into iPS cells and recovering clones which have modified allele in AAVS1 site by homologous recombination.
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Report
(3 results)
Research Products
(35 results)
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[Journal Article] Robust and highly-efficient differentiation of functional monocytic cells from human pluripotent stem cells under serum- and feeder cell- free conditions.2013
Author(s)
Yanagimachi MD, Niwa A, Tanaka T, Ozaki F, Nishimoto S, Murata Y, Yasumi T, Ito J, Tomida S, Oshima K, Asaka I, Goto H, Heike T, Nakahata T, Saito MK
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Journal Title
PLoS ONE
Volume: 8(4)
Issue: 4
Pages: e59243-e59243
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery2012
Author(s)
Tanaka T., Takahashi K., Yamane M., Tomida S., Nakamura S., Oshima K., Niwa A., Nishikomori R., Kambe N., Hara H., Mitsuyama M., Morone N., Heuse J.E., Yamamoto T., Watanabe A., Sato-Otsubo A., Ozawa S., Asaka I., Heike T., Yamanaka S., Nak
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Journal Title
Blood
Volume: 120(6)
Issue: 6
Pages: 1299-308
DOI
Related Report
Peer Reviewed
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[Journal Article] Multiple reversions of an IL2RG mutation restore combined immunodeficiency patient.2012
Author(s)
Kawai T., Saito M., Nishikomori R., Yasumi T., Izawa K., Murakami T., Okamoto N., Mori Y., Nakagawa N., Imai K., Nonoyama S., Wada T., Yatie A., Oomori K., Nakahata T., Heike T.
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Journal Title
J. Clin. Immunol
Volume: 32(4)
Issue: 4
Pages: 690-7
DOI
Related Report
Peer Reviewed
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[Journal Article] Detection of Base Substitution-Type Somatic Mosaicism of the NLRP3 Gene with> 99. 9% Statistical Confidence by Massively Parallel Sequencing2012
Author(s)
Izawa, K., A. Hijikata, N. Tanaka, T. Kawai, M. K. Saito, R. Goldbach-Mansky, I. Aksentijevich, T. Yasumi, T. Nakahata, T. Heike, R. Nishikomori, and O. Ohara
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Journal Title
DNA Res
Volume: 19
Issue: 2
Pages: 143-52
DOI
Related Report
Peer Reviewed
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