| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
The segmentation of pharyngeal arches requires Ripply, encoding an adaptor protein, which is expressed in the pharyngeal endoderm and ectoderm. Ripply3 is periodically expressed in caudal end of developing pharyngeal endoderm, where segmental morphogenesis takes place repeatedly. In this study, we identified a promoter element required for periodical expression of Ripply3 at the caudal end of pharyngeal endoderm by generating transgenic mice carrying Ripply3 promoter-EGFP fusion gene. We also showed that a feedback regulatory loop composed by Tbx1 and Ripply3 is critical for generating this periodicity by reporter analyses using culture cells and Ripply3 knock out mouse embryos. These results strongly suggest that a feedback interaction between Tbx1 and Ripply3 is important for periodical gene expression during segmentation of the pharyngeal endoderm, which has a slower periodicity than that in the segmentation of somites.
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