• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

An approach for finding of novel biological clock focusing on the segmentation of the pharyngeal arch

Research Project

Project/Area Number 23657153
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionOkazaki Research Facilities, National Institutes of Natural Sciences

Principal Investigator

TAKADA Shinji  大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (60206753)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords形態形成 / 分節形成 / 分子時計 / 咽頭弓 / 発生・分化 / 細胞・組織 / 遺伝子
Research Abstract

The segmentation of pharyngeal arches requires Ripply, encoding an adaptor protein, which is expressed in the pharyngeal endoderm and ectoderm. Ripply3 is periodically expressed in caudal end of developing pharyngeal endoderm, where segmental morphogenesis takes place repeatedly. In this study, we identified a promoter element required for periodical expression of Ripply3 at the caudal end of pharyngeal endoderm by generating transgenic mice carrying Ripply3 promoter-EGFP fusion gene. We also showed that a feedback regulatory loop composed by Tbx1 and Ripply3 is critical for generating this periodicity by reporter analyses using culture cells and Ripply3 knock out mouse embryos. These results strongly suggest that a feedback interaction between Tbx1 and Ripply3 is important for periodical gene expression during segmentation of the pharyngeal endoderm, which has a slower periodicity than that in the segmentation of somites.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (25 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) Remarks (3 results)

  • [Journal Article] Leucophores are similar to xanthophores in their specificatio and differentiation processes in medaka2014

    • Author(s)
      Kimura T, Nagao Y, Hashimoto H, Yamamoto-Shhiraishi Y, Yamamoto S, Yabe T, Takada S, Kinoshita M, Kuroiwa A, Naruse K
    • Journal Title

      Proc. Natl. Acd. Sci. USA

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional cooperation of spns2 and fibronectin in cardiac and lower jaw development2013

    • Author(s)
      Hisano Y, Ota S, Takada S, Kawahara A
    • Journal Title

      Biol Open

      Volume: 2 Pages: 789-794

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metameric pattern of intervertebral disc/vertebral body is generated independently of Mesp2/Ripply-mediated rostro-caudal patterning of somites in the mouse embryo2013

    • Author(s)
      Takahashi Y, Yasuhiko Y, Takahashi J, Takada S, Johnson RL, Saga Y, Kanno J
    • Journal Title

      Dev Biol

      Volume: 380 Pages: 172-184

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional cooperation of spns2 and fibronectin in cardiac and lower jaw development2013

    • Author(s)
      Yu Hisano, Satoshi Ota, Shinji Takada and Atsuo Kawahara
    • Journal Title

      Biology Open

      Volume: 2 Issue: 8 Pages: 789-794

    • DOI

      10.1242/bio.20134994

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Metameric pattern of intervertebral disc/vertebral body is generated independently of Mesp2/Ripply-mediated rostro-caudal patterning of somites in the mouse embryo.2013

    • Author(s)
      Takahashi Y, Yasuhiko Y, Takahashi J, Takada S, Johnson RL, Saga Y, Kanno J.
    • Journal Title

      Dev Biol.

      Volume: 380 Issue: 2 Pages: 172-184

    • DOI

      10.1016/j.ydbio.2013.05.020

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development and fibronectin signaling requirements of the zebrafish interrenal vessel2012

    • Author(s)
      Chiu CH, Chou CW, Takada S, Liu YW
    • Journal Title

      PLoS One

      Volume: 7(8)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mesogenin causes embryonic mesoderm progenitors to differentiate during development of zebrafish tail somites2012

    • Author(s)
      Yabe T. & Takada, S
    • Journal Title

      Dev. Biol

      Volume: 370 Pages: 213-222

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Loss of Porcupine impairs convergent extension during gastrulation in zebrafish2012

    • Author(s)
      Chen, Q Takada, R., & Takada, S
    • Journal Title

      J. Cell Sci

      Volume: 125 Pages: 2224-2234

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mesogenin causes embryonic mesoderm progenitors to differentiate during development of zebrafish tail somites2012

    • Author(s)
      Yabe T., ^*Takada' S.
    • Journal Title

      Dev. Biol.

      Volume: 370 Issue: 2 Pages: 213-222

    • DOI

      10.1016/j.ydbio.2012.07.029

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ripply 3, a Tbx repressor, is required for development of the pharyngeal apparatus and its its derivatives in mice2011

    • Author(s)
      Okubo, T., Kawamura, A., Takahashi, J., Yagi, H., Morishima, M., Matsuoka, R., & Takada, S
    • Journal Title

      Development

      Volume: 138 Pages: 339-348

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Negative feedback regulation of Ripply3 for own promoter during pharyngeal arch development2014

    • Author(s)
      Okubo T & Takada S
    • Organizer
      第47回日本発生生物学会
    • Place of Presentation
      ウィンク愛知 愛知県名古屋市
    • Related Report
      2013 Final Research Report
  • [Presentation] Negative feedback regulation of Ripply3 for own promoter during pharyngeal arch development2014

    • Author(s)
      Tadashi Okubo, Shinji Takada
    • Organizer
      第47回日本発生生物学会
    • Place of Presentation
      愛知県名古屋市 ウィング愛知
    • Related Report
      2013 Annual Research Report
  • [Presentation] Molecular mechanisms underlying segmentation border formation in zebrafish somitogenesis2013

    • Author(s)
      Wanglar C., Yabe, T. & Takada S
    • Organizer
      The 19th Japanese Medaka and Zebrafish Meeting
    • Place of Presentation
      仙台市情報産業プラザ 宮城県仙台市
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular mechanisms underlying segmentation border formation in zebrafish somitogenesis2013

    • Author(s)
      Wanglar C., Yabe, T. & Takada S
    • Organizer
      第46回日本発生生物学会
    • Place of Presentation
      くにびきメッセ 島根県松江市
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular mechanisms underlying segmentation border formation in Zebrafish somitogenesis2013

    • Author(s)
      Chimwar Wanglar, Taijiro Yabe, Shinji Takada
    • Organizer
      The 19th Japanese Medaka and Zebrafish Meeting
    • Place of Presentation
      宮城県仙台市 仙台市情報産業プラザ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Molecular mechanisms underlying segmentation border formation in zebrafish somitogenesis2013

    • Author(s)
      Chimwar Wanglar, Taijiro Yabe, Shinji Takada
    • Organizer
      第46回日本発生生物学会
    • Place of Presentation
      島根県松江市 くみびきメッセ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Jagged is required for epithelial reorganization2011

    • Author(s)
      Takada, S
    • Organizer
      Cell Symposium "Biology at the interface"
    • Place of Presentation
      RIKEN CDB 兵庫県神戸市
    • Year and Date
      2011-09-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Jagged/Notch directs epithelial morphogenesis2011

    • Author(s)
      Takada S
    • Organizer
      The 5th Asia and Oceania Zebrafish Meeting
    • Place of Presentation
      Beijing China
    • Year and Date
      2011-08-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Jagged/Notch directs epithelial reorganization2011

    • Author(s)
      Tsunokuni, H., Akanuma, T, & Takada, S
    • Organizer
      The 7th European Zebrafish Meeting
    • Place of Presentation
      Edingburgh, UK
    • Year and Date
      2011-07-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Jagged/Notch directs epithelial reorganization..2011

    • Author(s)
      Tsunokuni, H., Akanuma, T., Takada, S
    • Organizer
      he 7th European Zebrafish Meeting
    • Place of Presentation
      Edingburgh , UK
    • Related Report
      2011 Research-status Report
  • [Presentation] Jagged/Notch regulates epithelial morphogenesis..2011

    • Author(s)
      Shinji Takada
    • Organizer
      the 5th Asia -Oceania Zebrafish Meeting(招待講演)
    • Place of Presentation
      Beijing, China
    • Related Report
      2011 Research-status Report
  • [Presentation] Jagged is required for epithelial organization..2011

    • Author(s)
      Shinji Takada
    • Organizer
      Cell Symposium "Biology at the interface"(招待講演)
    • Place of Presentation
      理研CDB 神戸(兵庫県)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/cib2/

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/sections/developmental_biology/takada/

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://zfin.org/ZDB-LAB-050728-1

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi