• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional analysis of an insect sex determining gene found inSaccoglossus kowalevskii genome.

Research Project

Project/Area Number 23657154
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Research InstitutionThe University of Tokyo

Principal Investigator

SUZUKI Masataka  東京大学, 大学院・新領域創成科学研究科, 准教授 (30360572)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords性決定 / 性分化 / 選択的スプライシング / ギボシムシ / 進化 / 性決定遺伝子 / ミジンコ / 国際情報交流 / transformer遺伝子 / 半索動物
Research Abstract

We have found a gene (here after, depicted as Sktra) in Saccoglossus kowalevskii genome that is closely similar to a tra gene which is known to act as a central role in insect sex determination and determined its full-length cDNA sequence and its exon/intron structure. RT-PCR analyses demonstrated that, differ from the insect tra gene, no sex-specific splicing variants were observed in Sktra transcripts. The expression level of Sktra mRNA in testes is 9-fold higher than in ovaries. This result raises the possibility that Sktra is involved in sexual differentiation of S. kowalevskii. Splicing assays using insect culture cells revealed that Sktra can induce female-specific splicing of Drosophila dsx minigene pre-mRNA when co-transfected together with Sktra-2 cDNA. This result strongly suggests that the splicing activity specifically identified in insect Tra proteins is conserved in SkTra protein. We provide the world’s first evidence that demonstrates functional conservation of tra gene found in deuterostomes.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (6 results)

All 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] The expression and nuclear deposition of histone H3.1 in murine oocytes and preimplantation embryos.2012

    • Author(s)
      Kawamura M, Akiyama T, Tsukamoto S, Suzuki MG, Aoki F.
    • Journal Title

      Journal of Reproduction and Deveopment.

      Volume: Vol.58 Pages: 557-562

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Expression and Nuclear Deposition of Histone H3.1 in Murine Oocytes and Preimplantation Embryos2012

    • Author(s)
      Machika Kawamura
    • Journal Title

      Journal of Reproduction and Development

      Volume: 58 Pages: 557-562

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] カイコにおける遺伝的性決定機構2012

    • Author(s)
      鈴木雅京
    • Organizer
      日本進化学会第14回大会
    • Place of Presentation
      首都大学東京南大沢キャンパス(招待講演)
    • Year and Date
      2012-08-23
    • Related Report
      2012 Final Research Report
  • [Presentation] カイコにおける遺伝的性決定機構2012

    • Author(s)
      鈴木雅京
    • Organizer
      日本進化学会第14回大会
    • Place of Presentation
      首都大学東京南大沢キャンパス
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/seigyo/

    • Related Report
      2012 Final Research Report
  • [Remarks] なし

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi