Analysis of a new defense mechanism against microbial infections found in Paramecium
| Project/Area Number |
23657157
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 機能進化 / 細胞内共生 / ゾウリムシ / ホロスポラ / 感染防御 / 選択的食胞形成 / 一次共生 / 食胞形成 / 食胞形成忌避反応 / 生体防御 / 大核 |
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| Research Abstract |
Once Paramecium caudatum has been infected by endosymbiotic bacterium Holospora obtusa, the host cells become reduced the ability for digestive vacuole formation to the secondary added same bacteria, and that this change in the vacuole formation is induced within 4 h after the first infection. H. obtusa-bearing host cells can ingest various bacteria if the host has not yet been infected before by them. These suggest that Paramecium can distinguish and memory the infectious Holospora and acquires an unknown defense mechanism for the secondary infection. Treatment of the secondary added infectious H. obtusa with Lipase, Proteinase K, Lyticase, alpha-mannnosidase, or beta-galactosidase did not affect the host digestive vacuole formation. Also, treatments of the secondary added infectious H. obtusa and the host with antibodies for a 250-kDa cell surface protein of aposymbiotic paramecia are not effective. This phenomenon is repeatable, but molecular mechanism is still unknown.
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Report
(4 results)
Research Products
(39 results)
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[Presentation] 核内共生細菌ホロスポラの感染過程2013
Author(s)
籐島政博, 森川千穗, 藤瀬弘子, 栢 智昭, 岩谷綱一, 田中健也, 中村美紀, 児玉有紀
Organizer
中国四国地区生物系三学会合同大会(徳島大会)
Place of Presentation
徳島大学常三島キャンパス(徳島市)
Year and Date
2013-05-12
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[Book] Symbiotic associations between ciliates and prokaryotes. In, The Prokaryotes, 4th Edition, (Eds) Rosenberg E., DeLong E. F., Thompson F., Lory S., Stackebrand E., Quinones D.2013
Author(s)
Schweikert M., Fujishima M., Goertz H.-D.
Total Pages
37
Publisher
Springer-Verlag Berlin Heidelberg
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