Origin and evolution of allosteric riboswitches
| Project/Area Number |
23657160
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
TAMURA Koji 東京理科大学, 基礎工学部, 教授 (30271547)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 機能進化 / RNA / リボスイッチ / アロステリック |
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| Research Abstract |
The Bacillus subtilis glycine riboswitch comprises tandem glycine-binding aptamers and regulates transcription via the sensing of glycine. And it shows allosteric effect by binding of one glycine to one aptamer. Here, I found that the riboswitch behaves in a "glycine-independent" manner in the presence of polyethylene glycol and ethylene glycol. The effect is related to the formation of a terminator stem within the expression platform under such conditions. This provided new perspective about the evolution of riboswitch and acquisition of allostericity that molecule-specific functions of riboswitches would have come later, after the formation of less specific domain in riboswitches.
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Report
(4 results)
Research Products
(57 results)
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[Journal Article] Glycols modulate terminator stem stability and ligand-dependency of a glycine riboswitch2013
Author(s)
Hamachi, K., Hayashi, H., Shimamura, M., Yamaji, Y., Kaneko, A., Fujisawa, A., Umehara, T. and Tamura, K
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Journal Title
BioSystems
Volume: 113
Issue: 2
Pages: 59-65
DOI
Related Report
Peer Reviewed
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[Journal Article] RNA tetraplex as a primordial peptide synthesis scaffold2012
Author(s)
Umehara, T., Kitagawa, T., Nakazawa, Y., Yoshino, H., Nemoto, R. and Tamura, K
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Journal Title
BioSystems
Volume: 109
Issue: 2
Pages: 145-150
DOI
Related Report
Peer Reviewed
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