| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Research Abstract |
The Bacillus subtilis glycine riboswitch comprises tandem glycine-binding aptamers and regulates transcription via the sensing of glycine. And it shows allosteric effect by binding of one glycine to one aptamer. Here, I found that the riboswitch behaves in a "glycine-independent" manner in the presence of polyethylene glycol and ethylene glycol. The effect is related to the formation of a terminator stem within the expression platform under such conditions. This provided new perspective about the evolution of riboswitch and acquisition of allostericity that molecule-specific functions of riboswitches would have come later, after the formation of less specific domain in riboswitches.
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