| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
In Closterium peracerosum-strigosum-littorale complex, two types of conjugation produce zygotes; one is a conjugation between two complementary mating-type cells (mt+ and mt-) and the other is a conjugation between clonal cells. The former is referred to as heterothallism and the latter as homothallism.
Heterothallic mating group II-B and homothallic strains (naga37) are phylogenetically closely related. The formation of hybrid zygotes between the homothallic cells and either heterothallic cells was confirmed. These results suggest that at least some of the homothallic gametangial cells possess the same characteristics as heterothallic mt- and/ or mt+ cells. On the other hand, progenies derived from the hybrid zygotes could not be obtained.
Total RNA was collected from cells in several asexual and sexual reproductive stages and large scale RNAseq data was obtained by using new generation sequencer Hiseq2000. Finally, 132329 transcripts including many isoforms were established.
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